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Pharmingen apc brdu kit

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The BD Pharmingen™ APC BrDU Kit is a laboratory equipment product designed for the detection and analysis of cell proliferation. It provides the necessary reagents to measure bromodeoxyuridine (BrdU) incorporation into cellular DNA, which is a common method for evaluating cell division and proliferation.

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Lab products found in correlation

Facscalibur, by BD (1 mentions)

Close spelling variants of this product

BD Pharmingen (37 citations) APC BrdU kit (17 citations) BD PharmingenTM APC (2 citations)

2 protocols using pharmingen apc brdu kit

1

BrdU Incorporation Assay in Bone Marrow

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The BD Pharmingen™ APC BrDU Kit (BD Biosciences, #552598) was used as per the manufacturer’s protocol. 1 mg/mouse BrdU (BD bioscience, #552598) was administered intraperitoneally at 8 weeks after primary transplant, and bone marrow was harvested after 2 hours. Following ACK lysis, cells were stained for surface antigens, fixed and treated with DNase to expose BrdU antigen, and incubated with Pacific Blue conjugated anti-BrdU antibody as per the instruction manual. Flow cytometry was performed as described above.
Jalnapurkar S.S., Pawar A., George S.S., Antony C., Grana J., Gurbuxani S, & Paralkar V.R. (2024). PHF6 suppresses self-renewal of leukemic stem cells in AML. bioRxiv.
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2

BrdU Incorporation Assay in Keratinocytes

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Keratinocytes were seeded at low confluence in 50/50 media and allowed to expand for 24 hours. Cells were pulsed with 10ul of 1mM BrdU per 1 mL of culture media for 6 hours. Cells were trypsinzed in 0.05% Trypsin+EDTA for 5 minutes then quenched with ice-cold DMEM + 10% FCS and centrifuged at 500xg at 4°C. The pellet was resuspended in PBS + 1% FCS and again centrifuged at 500xg at 4°C. Fixation, permeabilization and anti-BrdU staining was performed as outlined in the BD Pharmingen APC BrdU Kit (BD Pharmingen). Stained cells were interrogated on BD FACSCalibur. Analysis was performed using FlowJo software (Tree Star, Oregon).
Bao X., Siprashvili Z., Zarnegar B., Shenoy R., Rios E., Nady N., Qu K., Mah A., Webster D., Rubin A., Wozniak G., Tao S., Wysocka J, & Khavari P.A. (2017). CSNK1a1 Regulates PRMT1 to Maintain the Progenitor State in Self-renewing Somatic Tissue. Developmental cell, 43(2), 227-239.e5.
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