Acrylamide, ammonium per sulfate, bovine serum albumin, calf thymus deoxyribonucleic acid (DNA), chloramine-T D-glucuronic acid and L-hydroxyproline were from Sigma Chemical Company, St. Louis, USA. p-dimethyl aminobenzaldehyde and Folin's Phenol reagent were from Loba Chemie, Mumbai, India. Methyl cellosolve was obtained from Merck, Darmstadt, Germany. All other reagents were high analytical grade.
Folin s phenol reagent
Manufactured by Loba Chemie
Sourced in India
Folin's Phenol reagent is a commonly used solution for the colorimetric determination of phenolic compounds. It is a mixture of phosphomolybdic and phosphotungstic acid complexes that react with phenols, resulting in a blue-colored complex that can be measured spectrophotometrically.
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2 protocols using folin s phenol reagent
1
Analytical Biochemistry Reagents Protocol
2
Tannin Quantification in Plant Extracts
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The total amount of tannins in the POE was obtained using the method of Giner-Chavez et al. (1997) (link) with minor modifications. Tannic acid (TA) (Analytical Grade, Scharlau, Spain) was used as the standard. POE (3.0 mL, 4,500 ppm) was mixed with 1.50 mL of Folin's phenol reagent (Loba Chemie Pvt. Ltd., India) and 9.0 mL of 35% (w/v) Na2CO3 (Analytical Grade, RCl Labscan, Thailand). After the reaction mixture was incubated for 5 min at room temperature, the absorbance of the complex formed was read at 740 nm ( max ) against the blank using the UV-Vis. The TTC was determined through regression analysis from the calibration curve of the TA (1.0-20 ppm with 5.0ppm increment) expressed as TA equivalence (TAE). The TTC was computed using Equation 2.
(1)
where C is the concentration of the GA obtained from the standard calibration curve (mg/L); DF is the dilution factor; V is the volume of the extract solution (L); m is the weight of the dried extract (g).
(1)
where C is the concentration of the GA obtained from the standard calibration curve (mg/L); DF is the dilution factor; V is the volume of the extract solution (L); m is the weight of the dried extract (g).
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