Dulbecco s pbs

Thrombin from human plasma and apo-transferrin were purchased from Sigma-Aldrich (USA). BSA and fibrinogen from human plasma were purchased from Wako Pure Chemicals Industries (Japan). All ssDNAs, except for the random ssDNA library with its primer set, were synthesized by Sigma-Aldrich (USA). 2-Morpholinoethanesulfonic acid (MES; Wako, Japan), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimido hydrochloride (EDC; Wako, Japan), Dulbecco’s PBS(−) (Wako, Japan), 10% Tween 20 solution (Bio-Rad, USA), tris(hydroxymethyl)aminomethane (Tris; Wako, Japan), 1 M sodium chloride solution (Wako, Japan), 1 M magnesium chloride solution (Wako, Japan), 1 M sodium hydroxide solution (Agilent Technologies, USA), 0.5 M borate buffer at pH 8.5 ± 0.2 (Polysciences, USA), EDTA disodium salt dihydrate (Wako, Japan), and boric acid (Wako, Japan) were used as received. All solutions were prepared using ultrapure water from a Milli-Q water purification system (Merck Millipore, USA). For the preparation of gels, a 37.5:1 (40%, w/v) acrylamide/bis solution, 2.6% C (Serva Electrophoresis, Germany), ammonium persulphate (Bio-Rad, USA), and N,N,N’,N’-tetramethylethylene (TEMED; Bio-Rad, USA) were used. The loading buffer and a 25-bp DNA stepladder were purchased from Wako (Japan).

For chemical stimulation, acetylcholine chloride (011-00592, FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) was used. It was dissolved in Dulbecco’s PBS (048-29805, FUJIFILM Wako Pure Chemical Corporation) to the desired concentration which avoids expansion of tissue due to osmotic pressure. PBS is widely used in experiments treating tissue.