Af488 conjugated donkey anti mouse igg2b

Manufactured by Thermo Fisher Scientific

Sourced in United States

AF488-conjugated donkey-anti-mouse IgG2b is a secondary antibody that is conjugated with the Alexa Fluor 488 fluorophore. It is designed to detect and bind to mouse IgG2b primary antibodies in immunoassays and other applications requiring fluorescent detection.

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Lab products found in correlation

Enoxaparin, by Sanofi (2 mentions) Dalteparin, by Pfizer (2 mentions) Fitc conjugated goat anti mouse igm, by Thermo Fisher Scientific (2 mentions) Cd1a apc mouse igg1, by BD (2 mentions) Heparan sulfate clone f58 10e4, by AMS Biotechnology (2 mentions) Facscanto 2, by BD (1 mentions) Mouse igg2a, by Abcam (1 mentions) Hla dr fitc clone g46 6, by BD (1 mentions) Mouse igg2b, by BD (1 mentions)

Spelling variants of this product

IgG2b (30 citations) Anti-mouse-AF488 (19 citations) Donkey anti-mouse AF488 (15 citations) Mouse IgG2b (9 citations) Anti-mouse IgG2B ( (4 citations)

2 protocols using af488 conjugated donkey anti mouse igg2b

SARS-CoV-2 Antibodies and Heparin Interactions

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The following antibodies were used (all anti‐human): ACE2 (R&D), (Heparan Sulfate (clone F58‐10E4) (Amsbio), digested Heparan (clone F69‐3G10) (Amsbio), CD1a‐APC mouse IgG1 (BD Biosciences, San Jose, CA, USA), CD207‐PE (langerin) mouse IgG1 (#IM3577), PerCP‐Cy™5.5‐conjugated mouse IgG1 EPCAM 347199) (BD Bioscience), PE‐conjugated mouse IgG1 E‐Cadherin (FAB18381P) (R&D Systems), APCcy‐conjugated mouse IgG1 CD45 (557833) (BD Bioscience), APC‐conjugated CD14 (21620146sp) (Immunotools), PE‐conjugated mouse IgG1 CD11b (101208) (Biolegend). FITC‐conjugated goat‐anti‐mouse IgM (#31992) (Invitrogen), AF488‐conjugated donkey‐anti‐mouse IgG2b (Invitrogen). Flow cytometric analyses were performed on a BD FACS Canto II (BD Biosciences). Data were analyzed using FlowJo vX.0.7 software (TreeStar).
The following reagents were used: unfractionated (UF) heparin, 5.000 IE/ml (LEO). low molecular weight heparins (LMWH): dalteparin, 10.000 IE anti‐Xa/ml (Pfizer), tinzaparin, 10.000 IE anti‐X1/0.5 ml (LEO), enoxaparin, 6000 IE (60 mg)/0.6 ml (Sanofi), nadroparin, 9.500 IE anti‐XA/ml (Aspen). Heparinase III from Flavobacterium heparium, EC 4.2.2.8, Batch 010, (Amsbio). Biotinylated SARS‐CoV‐2 S protein as well as neutralizing and non‐neutralizing COVA antibodies was generated as described previously (Brouwer et al, 2020 (link)).

Bermejo‐Jambrina M., Eder J., Kaptein T.M., van Hamme J.L., Helgers L.C., Vlaming K.E., Brouwer P.J., van Nuenen A.C., Spaargaren M., de Bree G.J., Nijmeijer B.M., Kootstra N.A., van Gils M.J., Sanders R.W, & Geijtenbeek T.B. (2021). Infection and transmission of SARS‐CoV‐2 depend on heparan sulfate proteoglycans. The EMBO Journal, 40(20), e106765.

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Multiparametric Flow Cytometry for Glycosaminoglycans

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The following antibodies were used (all anti-human): Heparan Sulfate (clone F58-10E4) (Amsbio), digested Heparan (clone F69-3G10) (Amsbio), Syndecan 1 (DL-101) (Santa Cruz), Syndecan 2-FITC (H-7) (Santa Cruz), Syndecan 3 (M-300) (Santa Cruz), Syndecan 4 (clone F94-8G3), CD207-PE (langerin) mouse IgG1 (#IM3577) (BeckmanCoulter, USA), CD1a-APC mouse IgG1 (BD Biosciences, San Jose, CA, USA) CD1a-PE (clone SK9) mouse IgG2b (BD Bioscience), HLA-B27-FITC (clone HLA-ABC-m3), mouse IgG2a (Abcam), HLA-DR-FITC (clone G46-6), mouse IgG2b (BD Bioscience), CD80-PE, mouse IgG1 (BD Pharmingen), CD83-PE, mouse IgG1 (eBioscience), CD86-FITC, mouse IgG1 (BD Pharmingen), FITC-conjugated goat-anti-mouse IgM (#31992) (Invitrogen), AF488-conjugated goat-anti-mouse IgG1 (#A21121) (Invitrogen), AF488-conjugated donkey-anti-mouse IgG2b (Invitrogen).
The following reagents were used: Unfractionated (UF) heparin, 5.000 I.E./ml (LEO). Low Molecular Weight (LMW) heparins: dalteparin, 10.000 IE anti-Xa/ml (Pfizer), tinzaparin, 10.000 IE anti-X1/0.5ml (LEO), enoxaparin, 100 mg/ml (Sanofi). 4-Nitrophenyl β-D-xylopyranoside (PNP-Xyl, 2001-96-9) (SigmaAldrich). Heparinase III from Flavobacterium heparium, EC 4.2.2.8, Batch 010 (Amsbio). 123Count eBeads, REF# 01-1234-42, LOT# E133305, 1.011.000 eBeads/ml (eBioscience).

Nijmeijer B.M., Eder J., Langedijk C.J., Kaptein T.M., Meeussen S., Zimmermann P., Ribeiro C.M, & Geijtenbeek T.B. (2020). Syndecan 4 Upregulation on Activated Langerhans Cells Counteracts Langerin Restriction to Facilitate Hepatitis C Virus Transmission. Frontiers in Immunology, 11, 503.

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