Single cell suspensions of peritoneal exudate cells were stained with primary antibodies including anti-CD11c eFluor610 (Catalog # 61011482, eBioscience, San Diego, CA), anti-MHCII AlexaFluor 647 (Catalog # 107618, BioLegend, San Diego, CA), anti-F4/80 Brilliant Violet 711 (Catalog # 123147, BioLegend), and anti-Ly-6G PE/Cy7 (Catalog #127618, BioLegend) for 20 min at 4°C in FACS buffer (1% BGS/ 0.01% NaN3 in PBS). Cells were then fixed in 3.7% formaldehyde (MilliporeSigma, Burlington, MA) for 15 min and then permeabilized in permeabilization buffer (0.1% saponin in PBS). Intracellular T. gondii tachyzoites were detected by staining with monoclonal anti-T. gondii (TP3) AlexaFluor 700 (Catalog # NB1102570, Novus Biologicals, Centennial, CO) for 45 min at 4°C in permeabilization buffer. All samples were run on a four laser (violet, blue, yellow, red) Attune NxT flow cytometer (ThermoFisher Scientific) and the data were analyzed using FlowJo v.10 software (FlowJo, Ashland, OR).
Alexafluor 700
Manufactured by Novus Biologicals
Sourced in United States
AlexaFluor 700 is a fluorescent dye used in biological research applications. It has an excitation maximum at 702 nm and an emission maximum at 723 nm, making it suitable for use in flow cytometry and other fluorescence-based detection methods.
Automatically generated - may contain errors
Lab products found in correlation
Anti ly6g pe cy7, by BioLegend (1 mentions)
Attune nxt flow cytometer, by Thermo Fisher Scientific (1 mentions)
Albumin bovine 5 bsa, by Solarbio (1 mentions)
Irdye 680 goat anti mouse igg, by LI COR (1 mentions)
Tris hydroxymethyl aminomethane tris, by Sangon (1 mentions)
Human serum albumin (hsa), by Solarbio (1 mentions)
2 protocols using alexafluor 700
1
Quantifying Intracellular Toxoplasma gondii
2
PMMA-Based Immunoassay Development
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Polymethyl methacrylate (PMMA) sheets with different thickness were purchased from XinTao Acrylic (ShenZhen, China). Double-sided adhesive tape with a thickness of 60 μm was purchased from Wenhao (Suzhou, China). Glutaraldehyde (GA, 50% in water) was purchased from Aladdin Reagent (Shanghai, China). Poly(ethylenimine) (PEI, Mw = 70000 g mol -1 , 50 wt% aqueous solution), human immunoglobulin (IgG) and protein A from Staphylococcus aureus (SPA) were purchased from Sigma-Aldrich (MT, USA). Human AFP recombinant protein was purchased from ARP Inc. (USA). Goat anti-Chicken IgY (IgG) H&L (Alexa Fluor ® 680) and Rabbit antichicken IgG were purchased from Abcam (England). Goat antimouse IgG (IRDye ® 680) was purchased from Li-Cor (NE, USA). Rabbit anti-human AFP antibody was purchased from Abcam (England). Albumin bovine V (BSA) and human serum albumin (HSA) were purchased from Solarbio (Beijing, China). Mouse anti-human AFP antibody (C3) (Alexa Fluor ® 700) was purchased from Novusbio (USA). Fetal bovine serum (FBS) was purchased from Lonsa (South America). Human fibrinogen (HF), mouse IgG (H+L), chicken IgY (IgG) (H+L), ready-touse PBS powder and tris(hydroxymethyl)aminomethane (Tris) were purchased from Sangon Biotech (Shanghai, China).
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