Log In Sign up
The largest database of trusted experimental protocols

Mycotect kit

Manufactured by Thermo Fisher Scientific
Visit Supplier

The MycoTect Kit is a laboratory product designed for the detection of mycoplasma contamination in cell cultures. It provides a reliable and efficient method for identifying the presence of mycoplasma in samples.

Automatically generated - may contain errors

Lab products found in correlation

Ampf str identification kit, by Thermo Fisher Scientific (3 mentions) Vero 76, by American Type Culture Collection (2 mentions) Rpmi 1640 medium, by American Type Culture Collection (2 mentions) Dulbecco modified eagle medium (dmem), by Corning (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) 1587 cercopithecus aethiops, by American Type Culture Collection (1 mentions) Mccoy s 5a modified medium, by Thermo Fisher Scientific (1 mentions) Hct116 cell, by American Type Culture Collection (1 mentions) Fetal calf serum (fcs), by Merck Group (1 mentions) Fetal bovine serum (fbs), by Corning (1 mentions) Atcc ccl 171, by American Type Culture Collection (1 mentions) Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions) A549 ccl 185, by American Type Culture Collection (1 mentions) H460 htb 177, by American Type Culture Collection (1 mentions) H1299 crl 5803, by American Type Culture Collection (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions) Ampf str identifier kit, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)

10 protocols using mycotect kit

1

Coxsackievirus and Poliovirus Propagation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell lines were purchased from American Type Culture Collection (ATCC). Cell lines supporting the multiplication of CV-B5 and Sb-1 are Monkey kidney (Vero-76) [ATCC CRL 1587 Cercopithecus Aethiops]. Viruses were purchased from the American Type Culture Collection (ATCC). Picornaviridae: Coxsackievirus [coxsackie type B5 (CV-B5), strain Faulkner (ATCC VR-185)], and human enterovirus C [poliovirus type-1 (Sb-1), Sabin strain Chat (ATCC VR-1562)]. Cell cultures were checked periodically for the absence of mycoplasma contamination with MycoTect Kit (Gibco). Viruses were maintained in our laboratory and propagated in appropriate cell lines. The viruses were stored in small aliquots at −80 °C until use.
Ibba R., Piras S., Corona P., Riu F., Loddo R., Delogu I., Collu G., Sanna G., Caria P., Dettori T, & Carta A. (2021). Synthesis, Antitumor and Antiviral In Vitro Activities of New Benzotriazole-Dicarboxamide Derivatives. Frontiers in Chemistry, 9, 660424.
+ Open protocol
+ Expand
2

HCT-116 Colon Cancer Cell Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols
HCT-116 cells (ATCC# CCL-247), derived from human colon carcinoma, were obtained from the American Type Culture Collection (ATCC, Manassas, VA). The cells were maintained in monolayer culture in McCoy’s 5A modified medium (Gibco BRL, Grand Island, NY) supplemented with 10 % fetal bovine serum (FBS), 55 μg/ml streptomycin and 55 IU/ml penicillin. The cells were grown until 5 days to 70–80 % confluence at 37 °C in a humidified atmosphere of 5 % CO2. The cell line tested negative for mycoplasma (MycoTect® kit, Gibco BRL) at regular intervals.
Bellamkonda K., Chandrashekar N.K., Osman J., Selvanesan B.C., Savari S, & Sjölander A. (2016). The eicosanoids leukotriene D4 and prostaglandin E2 promote the tumorigenicity of colon cancer-initiating cells in a xenograft mouse model. BMC Cancer, 16, 425.
+ Open protocol
+ Expand
3

Cryogenic Storage of A549 Lung Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human lung epithelial cell line (A549) was obtained as a gift from Dr. Huijun Zhu. Passage number is 76–79 according to ATCC website (http://atcc.custhelp.com/app/answers/detail/a_id/3/~/high-passage-number, 27/06/2014). Before using, bacterial, yeast and fungal contamination were checked under a microscope for mycoplasma using Gibco’s Mycotect kit (Cat.No. 15672017). A549 cells were cultured in F12/DMEM (Dulbecco’s Modified Eagle Medium) (Invitrogen, United Kingdom) supplemented with 10% fetal calf serum (sigma, United Kingdom) at 37 °C in 5% CO2. All the experiments were conducted using the same passage number. Six T-75 flasks of cells were collected and re-suspended in freezing media (90% serum + 10% dimethylsulfoxide), and then they were aliquoted into 1 ml vials. The cells were frozen and kept at −20 °C for 2 h and then at −80 °C overnight. The cells were then moved to a −150 °C freezer the next day. This protocol was repeated in each passage.
Zhou L., Forman H.J., Ge Y, & Lunec J. (2017). Multi-walled carbon nanotubes: A cytotoxicity study in relation to functionalization, dose and dispersion. Toxicology in vitro : an international journal published in association with BIBRA, 42, 292-298.
+ Open protocol
+ Expand
4

Cell Line Maintenance and Validation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mec1 (a kind gift from Dr. Maria Teresa S Bertilaccio, MD Anderson Cancer Center), Jurkat, (ATCC) and Mino (a kind gift from Dr. Hesham Amin, MD Anderson Cancer Center) cell lines were maintained in RPMI 1640 medium (ATCC) supplemented with 10% or 20% FBS (Life Technologies). A-T fibroblasts (A-T GM16666; and WT GM16667) were obtained from Coriell Cell Repositories and maintained in DMEM (Cellgro) supplemented with 10% FBS. All media were supplemented with 1% penicillin-streptomycin (Invitrogen Inc.). These cell lines were routinely tested for Mycoplasma using a MycoTect kit (Gibco), and all cell lines were validated using an AmpF/STR identification kit (Applied Biosystems) in the MD Anderson Cancer Center Cytogenetics and Cell Authentication Core. Routine cell number and the mean cell volume were determined by Coulter channelyzer (Coulter Electronics, Hialeah, FL).
Sarkar A, & Gandhi V. (2020). Activation of ATM kinase by ROS generated during ionophore-induced mitophagy in human T and B cell malignancies. Molecular and cellular biochemistry, 476(1), 417-423.
+ Open protocol
+ Expand
5

Cytotoxicity Evaluation of H. bella EOs

Check if the same lab product or an alternative is used in the 5 most similar protocols
MRC-5 cells, human lung fibroblasts from normal tissue [ATCC CCL-171], and Vero 76, Monkey kidney [ATCC CRL 1587] were purchased from American Type Culture Collection (ATCC, Manassas, Virginia, USA). Cell cultures were checked periodically for the absence of mycoplasma contamination with MycoTect Kit (Gibco).
Human lung fibroblasts were seeded at 1x106 cells/mL in 96 well plates in Minimum Essential Medium with Earle’s salts (MEM-E) medium with L-glutamine, supplemented with 10% FBS, 0.025g/L kanamycin. Vero-76 cells were seeded at an initial density of 4 × 105 cells/mL in 96-well plates, in culture medium Dulbecco’s Modified Eagle Medium (D-MEM) with L-glutamine, supplemented with FBS, 0.025g/L kanamycin. Cells were then incubated at 37 °C in a humidified, 5% CO2 atmosphere in the absence or presence of serial dilutions of H. bella EOs. Cell viability was determined after 72 (MRC-5) and 96 (Vero 76) hrs at 37 °C by the 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) method [81 (link)]. Dimethyl sulfoxide (DMSO) was used as a control in each experiment, and it was tested at the maximum concentration present in each compound. The cytotoxicity of test compounds (100 μg/mL, the maximum concentration tested) was evaluated in parallel with their antiviral activity.
Donadu M.G., Trong Le N., Viet Ho D., Quoc Doan T., Tuan Le A., Raal A., Usai M., Marchetti M., Sanna G., Madeddu S., Rappelli P., Diaz N., Molicotti P., Carta A., Piras S., Usai D., Thi Nguyen H., Cappuccinelli P, & Zanetti S. (2020). Phytochemical Compositions and Biological Activities of Essential Oils from the Leaves, Rhizomes and Whole Plant of Hornstedtia bella Škorničk. Antibiotics, 9(6), 334.
+ Open protocol
+ Expand
6

NSCLC Cell Lines Maintenance Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
H460 (HTB177™), H1299 (CRL5803™) and A549 (CCL185™) human NSCLC cells were purchased from the American Type Culture Collection (Manassas, VA, USA) and maintained in Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich; Merck Millipore, Darmstadt, Germany). All cultured cells were supplemented with L-glutamine, sodium pyruvate and cultured with 10% heat-inactivated fetal bovine serum (FBS; Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA) in 5% CO2 at 37°C. All cell lines were periodically examined using a MycoTect™ kit (Invitrogen; Thermo Fisher Scientific, Inc.), to ensure the absence of mycoplasma contamination.
Wang J.P., Hsieh C.H., Liu C.Y., Lin K.H., Wu P.T., Chen K.M, & Fang K. (2017). Reactive oxygen species-driven mitochondrial injury induces apoptosis by teroxirone in human non-small cell lung cancer cells. Oncology Letters, 14(3), 3503-3509.
+ Open protocol
+ Expand
7

Characterization of MCL1-Deficient MEFs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse embryonic fibroblasts (MEFs), wild-type and MCL1 deficient were generously provided by Dr. Joseph T. Opferman at St. Jude Children's Research Hospital (Memphis, TN) [25 (link)] Both cell lines are Simian virus (SV40)-transformed and the cells were maintained in Dulbecco modified Eagle medium with L-glutamine (DMEM; Invitrogen) media supplemented with 10% fetal bovine serum (FBS; Invitrogen), Pen/Strep, L-Glut, and non-essential amino acids (NEAA; GIBCO). Presence or absence of Mcl-1 protein in these cells were confirmed by Dr. Opferman's group as well as by our group using immunoblots. Cell lines were periodically tested for Mycoplasma contamination using a MycoTect kit (Invitrogen). All experiments were conducted in cell passages less than 15 and were maintained at a logarithmic growth concentration between 105 cells/mL and 106 cells/mL with 80% confluency as determined by a Coulter channelyzer with less than 10% endogenous cell death confirmed by flow cytometry.
Modi P., Balakrishnan K., Yang Q., Wierda W.G., Keating M.J, & Gandhi V. (2017). Idelalisib and bendamustine combination is synergistic and increases DNA damage response in chronic lymphocytic leukemia cells. Oncotarget, 8(10), 16259-16274.
+ Open protocol
+ Expand
8

Breast Cancer Tissue and Cell Line Analysis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Two independent sets of breast cancers with matched protein lysates and formalin-fixed, paraffin-embedded (FFPE) sections were utilized: TCGA (n = 885) and MDACC (n = 63). For the TCGA cohort, only a subset of cases with RPPA data was available with matched unstained FFPE sections (n = 109).
Breast cancer cell lines (T47D, MCF7) were obtained from the MD Anderson Characterized Cell Line Core facility (Houston, TX). The identities of all cell lines were verified using AmpF/STR Identifier kit (Applied Biosystems) by the core facility and used within 6 months of obtaining the cell lines. The lines were cultured in DMEM supplemented with 5% fetal bovine serum (FBS) at 37°C in 5% carbon dioxide atmosphere. Cell lines were routinely tested for Mycoplasma infection using a MycoTect Kit (Invitrogen).
MDA-MB-134 (ATCC) and Sum44PE (Asterand) cells were cultured as previously described (16 (link)). The cell lines were authenticated annually by PCR RFLP analyses and confirmed to be Mycoplasma negative at the University of Pittsburgh Cell Culture and Cytogenetics Facility (Pittsburgh, PA).
Cancer tissues from the University of Utah (A.W.) were mastectomy specimens from patients without prior therapy. Normal specimens were collected from matched grossly uninvolved tissues. Tissues were collected and de-identified using approved IRB#10924.
Dennison J.B., Shahmoradgoli M., Liu W., Ju Z., Meric-Bernstam F., Perou C.M., Sahin A.A., Welm A., Oesterreich S., Sikora M.J., Brown R.E, & Mills G.B. (2016). High intra-tumoral stromal content defines Reactive breast cancer as a low-risk breast cancer subtype. Clinical cancer research : an official journal of the American Association for Cancer Research, 22(20), 5068-5078.
+ Open protocol
+ Expand
9

Maintenance of MCL and MEF Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
Three MCL cell lines (Granta-519, JeKo-1 and Mino) were used in this study. Granta-519 was maintained in Dulbecco modified Eagle medium with high glucose (DMEM, Cellgro) supplemented with 20% FBS while both Jeko-1 and Mino cell lines were cultured in RPMI 1640 medium (ATCC) supplemented with 20% FBS. Wild type, heterozygote and double knockout mouse embryonic fibroblasts for Casp3 and Casp7 (DKO MEF) were maintained through passage 10 in DMEM (Cellgro) supplemented with 10% FBS. All media were supplemented with 1% penicillin-streptomycin (Invitrogen Inc.). These cell lines were routinely tested for Mycoplasma using a MycoTect kit (Invitrogen). MCL cell lines were validated by AmpF/STR Identification kit (Applied Biosystems) in the MD Anderson cell line validation core facility. Routine cell number and the mean cell volume were determined by Coulter channelyzer (Coulter Electronics).
Sarkar A., Balakrishnan K., Chen J., Patel V., Neelapu S.S., McMurray J.S, & Gandhi V. (2015). Molecular evidence of Zn chelation of the procaspase activating compound B-PAC-1 in B cell lymphoma. Oncotarget, 7(3), 3461-3476.
+ Open protocol
+ Expand
10

Cell Line Verification and Preservation

Check if the same lab product or an alternative is used in the 5 most similar protocols
MCL cell lines (JeKo-1, Mino and Granta 519) were obtained from Dr. Hesham Amin (Supplemental Table 1) (35 (link)–37 ). The cells were routinely tested for Mycoplasma using a MycoTect kit (Invitrogen), and they were authenticated using an AmpF/STR Identification kit (Applied Biosystems, Foster City, CA). Stocks of authenticated cell lines were stored in −80C for future use, and all cell lines used in this manuscript were from these authenticated stocks.
Yang Q., Chen L.S., Ha M.J., Do K.A., Neelapu S.S, & Gandhi V. (2016). Idelalisib impacts cell growth through inhibiting translation regulatory mechanisms in mantle cell lymphoma. Clinical cancer research : an official journal of the American Association for Cancer Research, 23(1), 181-192.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!