Three types of microscopes were used for imaging purposes. Larger glass beads were imaged using low magnification (maximum 10x) microscope (Lawrence & Mayo, India). Samples with smaller glass beads were viewed using a high-resolution microscope (Carl Zeiss AG, Germany); the magnifications used were 100x and 200x. In the third type, we used a scanning electron microscope for further magnification and better contrast. Along with these three standard types of microscopy, we also used digital cameras (Nikon P7800 and Canon A2600) for imaging purposes.
A2600
Manufactured by Canon
The Canon A2600 is a compact and versatile lab equipment designed for general laboratory use. It features an integrated touchscreen display for intuitive operation and provides essential functionalities for various laboratory tasks.
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Lab products found in correlation
2 protocols using a2600
1
Multi-Scale Microscopy Imaging Protocol
2
Monitoring Pupillary Dilation in Birds
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The beginning of the collection of images for monitoring pupillary dilation of the animals occurred after the third week of age of the birds, measured weekly at experimental temperatures, during the period when the chambers remained closed. The images were captured by a Canon® model A2600 camera with 16 Mp of resolution, with flash disabled, positioned so as to capture the images of the animals' pupils.
Reference scales were fixed in the animals' beak (Figures 1 and2), previously adapted to this situation, with 5 mm intervals to allow the conversion of the pupil dimensions that were collected in the images in pixel scale and later converted to millimeters in its real dimensions. The pupil temperature of the animals was monitored by capturing thermograms from the eyeball, using a thermographic camera Flir ® model TG-165. The thermal images were collected weekly in each proposed treatment. The average pupil temperature of the animals was obtained from the thermograms in the two thermal conditions evaluated, adopting an emissivity of 0.98 (emissivity of biological tissue).
Reference scales were fixed in the animals' beak (Figures 1 and2), previously adapted to this situation, with 5 mm intervals to allow the conversion of the pupil dimensions that were collected in the images in pixel scale and later converted to millimeters in its real dimensions. The pupil temperature of the animals was monitored by capturing thermograms from the eyeball, using a thermographic camera Flir ® model TG-165. The thermal images were collected weekly in each proposed treatment. The average pupil temperature of the animals was obtained from the thermograms in the two thermal conditions evaluated, adopting an emissivity of 0.98 (emissivity of biological tissue).
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