P24 kc57 fitc

Fixable Viability Stain 510 was obtained from ThermoFisher Scientific (#L34957; 1/1000 dilution). The following antibodies were used in sorting experiments: CD8-AF700 Clone RPA-T8 (ThermoFisher, #56-0088-41; 1/200 dilution), CD45RO-BV421 Clone UCHL1 (BD Biosciences, #562649; 1/100 dilution), CD27-BV605 Clone L128 (BD Biosciences, #562656; 1/100 dilution). For p24 staining, we used a combination of two antibodies: p24 KC57-FITC (Beckman Coulter, #6604665; 1/500 dilution) and p24 28B7-APC (MediMabs, #MM-0289-APC; 1/400 dilution).

Fixable Viability Stain 510 was obtained from ThermoFisher Scientific (#L34957, 1/1000). The following antibodies were used in staining experiments: CD3 AF700 Clone UCHT1 (BD Biosciences, #557943, 1/50), CD4 PE-Cy7 Clone L200 (BD Bioscience, #560644, 1/200) and CD4 BV786 Clone SK3 (BD Biosciences, #563881, 1/200), CD8 BV510 Clone RPA-T8 (BioLegend, #301047, 1/200), CD45RO BV421 Clone UCHL1 (BD Biosciences, #562649, 1/100) or CD45RO PE Clone HI100 (BD Biosciences, #5555493, 1/20), CD27 BV605 Clone L128 (BD Biosciences, #562656, 1/100), PD1 BB700 Clone EH12.1 (BD Biosciences, #566461, 1/100), CD69 PerCP-Cy5.5 Clone FN50 (BD Biosciences, #560738) and CD69 APC (BioLegend, #310909, 1/50), CD25 BV421 Clone M-A251 (BD Biosciences, #562443, 1/50), HLA-DR FITC Clone REA805 (Miltenyi Biotech, #130-111-788, 1/50), GZMA PE-Cy7 Clone CB9 (BioLegend, #507221, 1/25), GZMB PB Clone GB11 (BioLegend, #515407, 1/50), CCL5 PerCP-Cy5.5 Clone VL1 (BioLegend, #515507, 1/50), CD127 PE-CF594 Clone HIL-7R-M21 (BD Biosciences, #562397, 1/50). For p24 staining, we used a combination of two antibodies: p24 KC57-FITC (Beckman Coulter, #6604665, 1/500) and p24 28B7-APC (MediMabs, #MM-0289-APC, 1/400).