Platelets or PMNs were fixed and incubated with antibodies including anti-NLRP3 (Abcam, 1:100, catalog no. ab4207), anti-ASC (Novusbio, 1:100, catalog no. NBP1-78978), anti-CD41 (Abcam, 1:200, catalog no. ab134131), Alexa Fluor 488 anti-mouse CD41 (BioLegend, 1:200, catalog no. 133908), Hoechst (Thermo Fisher Scientific, 1:3,000, catalog no. H3570), anti-myeloperoxidase (Genetx, 1:200, catalog no. gtx75318), anti-myeloperoxidase (Abcam, 1:200, catalog no. ab208670) and anti-histone H3 (citrulline R2 + R8 + R17, 1:200, Abcam, catalog no. ab5103) at 4 °C overnight. Secondary antibodies included Alexa Fluor 647-conjugated anti-goat antibody (Abcam, 1:200, catalog no. ab150135), Alexa Fluor 647-conjugated anti-rabbit IgG antibody (Abcam, 1:200, catalog no. ab150079), Alexa Fluor 594-conjugated anti-rabbit IgG antibody (Abcam, 1:200, catalog no. ab150084), Alexa Fluor 594-conjugated anti-mouse IgG antibody (Abcam, 1:200, catalog no. ab150116), Alexa Fluor 488-conjugated anti-goat IgG antibody (BioLegend, 1:200, catalog no. 405508), Alexa Fluor 488-conjugated anti-rabbit IgG antibody (Abcam, 1:200, catalog no. ab150077) and Alexa Fluor 488-conjugated anti-mouse IgG antibody (Abcam, 1:200, catalog no. ab150113). Cells were captured using immunofluorescence confocal microscopy (×63 oil immersion lens, Leica SP8).
Alexa fluor 594 conjugated anti mouse igg antibody
Manufactured by Abcam
Sourced in United Kingdom
Alexa Fluor 594-conjugated anti-mouse IgG antibody is a secondary antibody that specifically binds to mouse immunoglobulin G (IgG) molecules. The antibody is conjugated with the Alexa Fluor 594 fluorescent dye, which can be used for detection and visualization purposes in various immunoassays and imaging applications.
Automatically generated - may contain errors
Lab products found in correlation
Alexa fluor 488 conjugated anti rabbit igg antibody, by Abcam (2 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions)
Hoechst, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 594 conjugated anti rabbit igg antibody, by Abcam (1 mentions)
Alexa fluor 488 conjugated anti mouse igg antibody, by Abcam (1 mentions)
Anti cd41, by Abcam (1 mentions)
Anti nlrp3, by Abcam (1 mentions)
Mounting medium, by Agilent Technologies (1 mentions)
Lsm 880 laser scanning microscope, by Zeiss (1 mentions)
Mouse anti cgrp, by Abcam (1 mentions)
Rabbit anti laminin, by Abcam (1 mentions)
Dylight 649, by Vector Laboratories (1 mentions)
3 protocols using alexa fluor 594 conjugated anti mouse igg antibody
1
Immunofluorescence Analysis of Inflammasome Components
2
Immunofluorescent Quantification of γ-H2AX
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For γ-H2AX and 4′,6-diamidino-2-phenylindole (DAPI) staining, cells were fixed in 4% paraformaldehyde for 15 min at room temperature and permeabilized with 0.1% Triton X-100 in PBS for 20 min. The cells were then incubated with 1:200 dilution of anti-phosho-H2AX (Ser139) antibody overnight at 4 °C. AlexaFluor 594-conjugated anti-mouse IgG antibody (Abcam, Cambridge, UK) was used at 1:400 dilution for 1 h at room temperature. The slides were mounted in mounting medium (DAKO, Santa Clara, CA, USA) with DAPI (ThermoFisher Scientific) before imaging. Images were acquired using an LSM880 laser scanning microscope (ZEISS, Jena, Germany). Fluorescent images were captured using the appropriate filters. Images were analyzed using the Image J and ZEN software.
3
Immunolabeling of Spinal Cord and DRG Tissues
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
One month after dorsal root crush injury, rats were euthanized with pentobarbital sodium and perfused with 4% PFA. Under a stereomicroscope, spinal cords with attached roots and DRG tissues were collected from perfused rats. Subsequently, tissues were postfixed in 4% PFA for 24 h, and cryoprotected in 30% sucrose (wt/vol) for at least 48 h at 4 °C. The tissues were then embedded in OCT (Sakura, Finetek, USA), cryosectioned using a cryostat, and mounted onto slides. Spinal cord and DRG tissues were cryosectioned at thicknesses of 25 µm and 15 μm, respectively. After blocking in 5% BSA with 0.1% Triton X-100 for 1 h, sections were incubated with primary antibodies at 4 °C overnight. Secondary antibodies were applied for 2 h at room temperature. The following primary antibodies were used: mouse anti-CGRP (1:1000; Abcam); rabbit anti-laminin (1:2000; Abcam); and IB4 conjugated with DyLight 649 (1:500; Vector). The corresponding secondary antibodies were used as follows: Alexa Fluor 488-conjugated anti-rabbit IgG antibody (1:1000; Abcam) and Alexa Fluor 594-conjugated anti-mouse IgG antibody (1:1000; Abcam). Images were acquired by confocal microscopy (Zeiss 710) and analyzed using ImageJ software.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!