Cells were fixed with cold 70% ethanol, and stained with an APC conjugated anti-human Ki-67 antibody (Cat #: 350513, Biolegend) in PBS containing 2% FBS for 30 minutes at room temperature. When PCa cells were co-cultured with murine MC3T3-E1cells, cells were stained with a FITC-anti-HLA-ABC antibody (clone W6/32: Cat #: 311404, BioLegend) to identify human PCa cells before fixation. Ki-67 negative PCa cells were examined using a FACS Aria dual-laser flow cytometer.
Fitc anti hla abc antibody clone w6 32
Manufactured by BioLegend
The FITC-anti-HLA-ABC antibody (clone W6/32) is a fluorescently-labeled monoclonal antibody that binds to the human leukocyte antigen (HLA) class I molecules, which are expressed on the surface of most nucleated cells. This antibody can be used to detect and quantify HLA class I expression on cells in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using fitc anti hla abc antibody clone w6 32
1
Ki-67 Expression in PCa Co-Culture
2
BrdU Labeling and Flow Cytometry
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were cultured in the presence of 10 μM BrdU for 30 minutes, and then BrdU was detected following the manufacturer’s protocol (BrdU Labeling kit, Cat #: 552598, BD Biosciences, San Jose, CA). Cells were also stained with a FITC-anti-HLA-ABC antibody (clone W6/32: Cat #: 311404, BioLegend, San Diego, CA). PCa cells were gated as HLA-positive populations and BrdU-positive cells were detected by a FACS Aria dual-laser flow cytometer.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!