To determine circulating EPC numbers, MNCs isolated from peripheral blood (PB) were stained with FITC-conjugated anti-CD34 monoclonal antibody (GeneTex, Inc., USA) and PE-conjugated anti-VEGFR2 monoclonal antibody (BD Biosciences). ‘Early EPCs’ were stained with FITC-conjugated anti-CD14 (BD Pharmingen) or AlexaFluor® 647- anti-CD45.2 antibody (BioLegend). A FACScalibur unit (BD Biosciences) was used to detect fluorescent labeled cells.
Pe conjugated anti vegfr2 monoclonal antibody
Manufactured by BD
Sourced in United States
The PE-conjugated anti-VEGFR2 monoclonal antibody is a laboratory research tool used for the detection and analysis of VEGFR2 (Vascular Endothelial Growth Factor Receptor 2) in biological samples. It is a mouse monoclonal antibody that has been conjugated with the fluorescent dye R-Phycoerythrin (PE). This antibody can be used in various immunological techniques, such as flow cytometry, to identify and quantify VEGFR2-expressing cells.
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2 protocols using pe conjugated anti vegfr2 monoclonal antibody
1
Circulating Endothelial Progenitor Cells Quantification
2
Immunofluorescence Staining of Tumor Endothelial Cells
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Tumor masses or ‘early EPCs’ were fixed with 4% paraformaldehyde (PFA) in PBS. Sections were prepared from OCT embedded tumor tissues and washed in PBS. After blocking with 1% bovine serum albumin (BSA, Sigma) in PBS, sections were stained with rat anti-mouse CD31 antibody overnight at 4°C, and then with Alexa Flour 647 -conjugated anti-rat antibody (Invitrogen). Fixed cells were stained with FITC-conjugated anti-CD34 monoclonal antibody (GeneTex) and PE-conjugated anti-VEGFR2 monoclonal antibody (BD Biosciences).
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