Sekm 0002

Manufactured by Solarbio

Sourced in China

The SEKM-0002 is a piece of lab equipment designed for the analysis and separation of biological samples. It features a compact and durable construction, making it suitable for use in various research and testing environments.

Automatically generated - may contain errors

Lab products found in correlation

Sekm 0034, by Solarbio (6 mentions) Sekm 0007, by Solarbio (5 mentions) Sekh 0047, by Solarbio (1 mentions) Sekh 0013, by Solarbio (1 mentions) Sekh 0018, by Solarbio (1 mentions) Sekm 0019, by Solarbio (1 mentions) Flexstation 3, by Molecular Devices (1 mentions) Csb e10787m, by Cusabio (1 mentions) Csb e08300m, by Cusabio (1 mentions)

7 protocols using sekm 0002

Cytokine Profiling in Lung Tissues

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The levels of IL-6 (SEKM-0007 and SEKH-0013, Solarbio), tumor necrosis factor-α (TNF-α) (SEKM-0034 and SEKH-0047, Solarbio), IL-10 (SEKM-0010 and SEKH-0018, Solarbio), IL-18 (SEKH-0028 and SEKM-0019), and IL-1β (SEKM-0002 and SEKH-0002) in lung tissues and cell cultures were measured according to the instructions.

Chen J., Huang Y., Bian X, & He Y. (2022). Berberine Ameliorates Inflammation in Acute Lung Injury via NF-κB/Nlrp3 Signaling Pathway. Frontiers in Nutrition, 9, 851255.

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Measurement of Inflammatory Biomarkers

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ELISA kits for the detection of Lp-PLA2 (CSB-E08321m, Cusabio, USA), TNF-α (SEKM-0034, Solarbio, China), IL-6 (SEKM-0007, Solarbio, China) and IL-1β (SEKM-0002, Solarbio, China) were obtained from Solarbio (Solarbio, Beijing, China). The levels of Lp-PLA2, TNF-α, IL-6 and IL-1β were analysed with corresponding ELISA kits according to the manufacturer's instructions. Then, the absorbance of the coloured product was measured at 405 nm by an automated plate reading system (FlexStation 3, Molecular Device, Sunnyvale, CA, USA). Each experiment was independently performed three times.

Yan W., Yan Y., Luo X., Dong Y., Liang G., Miao H., Huang Z, & Jiang H. (2024). Lipopolysaccharide (LPS)-induced inflammation in RAW264.7 cells is inhibited by microRNA-494-3p via targeting lipoprotein-associated phospholipase A2. European journal of trauma and emergency surgery : official publication of the European Trauma Society.

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Spinal Cord Cytokine Expression

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The protein expression of interleukin (il)-1β, tumor necrosis factor (TnF)-α and il-6 in lumbar dorsal spinal cord tissue homogenates were measured using commercially available eliSa kits (SEKM-0002, SEKM-0034 and SEKM-0007; Solarbio life Science), according to the manufacturer's protocols. The experiment was repeated three times, and the mean value was used.
Statistical analysis. SPSS (version 18.0; SPSS, inc.) was used for data analysis. data were presented as the mean ± standard deviation. comparisons between two groups were estimated by a Student's t-test, whereas multiple group comparisons were carried out by one-way anoVa analysis with a Bonferroni's post hoc test. P<0.05 was considered to indicate a statistically significant difference.

Zhou X.G., He H, & Wang P.J. (2020). A critical role for miR‑135a‑5p‑mediated regulation of SLC24A2 in neuropathic pain. Molecular medicine reports, 22(3).

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Cytokine Profiling in Serum

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The levels of interleukin 6 (IL-6) (PI326; Beyotime, Beijing, China), tumor necrosis factor-α (TNF-α) (SEKM-0034, Solarbio), interleukin 1β (IL-1β) (SEKM-0002, Solarbio) in serum were detected using ELISA kits.

Chen P., Liu W, & Wei Y. (2023). Ramelteon attenuates renal ischemia and reperfusion injury through reducing mitochondrial fission and fusion and inflammation. Translational Andrology and Urology, 12(12), 1859-1870.

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Measuring Inflammatory Markers and Cardiac Injury

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Mouse serum or HL-1 cell supernatant was collected. The levels of tumor necrosis factor α (TNF-α), interleukin (IL)-1β, IL-6, and cardiac troponin I (cTnI) were measured separately using the corresponding ELISA kits according to the manufacturer’s instructions. The kits included TNF-α (SEKM-0034; Solarbio), mouse IL-6 (SEKM-0007; Solarbio), IL-1β (SEKM-0002; Solarbio), and mouse cTnI (KS11280; Keshun Science and Technology, Shanghai, China).

Jiang Y., Xu J., Zeng H., Lin Z., Yi Q., Guo J, & Xiao F. (2024). miR-29b-1-5p exacerbates myocardial injury induced by sepsis in a mouse model by targeting TERF2: miR-29b-1-5p/TERF2 affect myocardial injury. Acta Biochimica et Biophysica Sinica, 56(4), 607-620.

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ELISA Quantification of Alzheimer's Biomarkers

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The concentrations of Aβ1-42 (CSB-E10787m, Cusabio, Hubei, China), Aβ1-40 (CSB-E08300m,Cusabio), IL-1β (SEKM-0002, Solarbio, Beijing, China), and TNF-α (SEKM-0034, Solarbio) were assessed by enzyme-linked immunosorbent (ELISA) assay kits. In brief, cells were seeded onto 96-well plates and treated under different conditions. Thereafter, 100 μL of supernatants was harvested to perform ELISA assays. Nitric oxide levels were detected according to total nitrate/nitrite ratios in the supernatants following the manufacturer’s instructions (Nitric Oxide Assay Kit, EMSNO, Pierce, Rockford, IL). Briefly, nitrate reductase and the enzyme cofactor were added to wells and incubated to convert nitrate to nitrite. Next, the enhancers were added and incubated at room temperature. Finally, the Griess reagents were added and the samples were analyzed with a microplate reader.

Zeng Y., Li Y., Shen H., Lin N, & Zhang J. (2021). Tripchlorolide attenuates β-amyloid generation by inducing NEP activity in N2a/APP695 cells. Translational Neuroscience, 12(1), 301-308.

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Cytokine Quantification in Lung Tissue

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Freshly prepared lungs were homogenized with a tissue homogenizer. The homogenate was placed in a benchtop refrigerated centrifuge and centrifuged at 3000 rpm for 20 min. The supernatant was collected after centrifugation. TNF-α (PT513, Solarbio Science & Technology Co., Ltd, Beijing, China), IL-1β (SEKM-0002, Solarbio Science & Technology Co., Ltd, Beijing, China) and IL-6 (SEKM-0007, Solarbio Science & Technology Co., Ltd, Beijing, China) assays were performed according to the instructions of the ELISA kit.

Ou Y., Fu Q., Chen Y., Lin L., Wang J., Wu D., Wu Q, & Xie J. (2023). Dietary Ochratoxin A Contamination Modulates Oxidative Stress, Inflammation Processes and Causes Fibrosis in in vitro and in vivo Lung Models. Frontiers in bioscience (Landmark edition), 28(2).

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