High performance liquid chromatography (HPLC) grade methanol, acetonitrile, acetone, hexane, and water were purchased from Sigma-Aldrich Corp., (St. Louis, MO, USA). Analytical standards of fipronil, and fipronil-desulfinyl were obtained from Sigma-Aldrich Corp., (St. Louis, MO, USA). Analytical standards of fipronil sulfide, –sulfone, and –amide were obtained from Bayer (Leverkusen, Germany) and BASF (Ludwigshafen, Germany). Labeled 13C215N2 fipronil, 13C415N2 fipronil sulfone, and 13C415N2 fipronil sulfide were purchased from Toronto Research Chemicals (Toronto, Ontario, Canada) and Cambridge Isotope Laboratories, Inc., (Tewksbury, MA, USA), respectively. Stock solutions of analytical standards were prepared in acetonitrile and stored at −20°C. Working calibration standards were prepared in water and methanol (50/50 v/v) with 0.1% formic acid. For those compounds without isotopically labeled standards available, quantitation was performed using the method of standard addition with 4 spike standard levels.
Fipronil sulfide
Manufactured by Bayer
Sourced in Germany, Switzerland
Fipronil sulfide is a laboratory reagent used in various analytical and research applications. It is a metabolite of the insecticide fipronil and is employed as a reference standard or analytical tool in laboratories. The core function of fipronil sulfide is to serve as a reference compound for identification, quantification, and method development purposes, particularly in the analysis of fipronil and related compounds.
Automatically generated - may contain errors
2 protocols using fipronil sulfide
1
Fipronil Pesticide Analysis by HPLC
2
Fipronil Residue Analysis in Soil
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Certified reference standards of fipronil (purity 98.4% w/w) procured from, Sigma Aldrich, Switzerland, fipronil desulfinyl, (purity 98.5% w/w), fipronil sulfide (purity 98% w/w/) and fipronil sulfone (purity 99.7% w/w) obtained from Bayer crop science, Germany were used as analytical standards. The solvents and other reagents viz., acetone, ammonia solution, dichloromethane (HPLC grade), methanol, acetonitrile (LC-MS/MS grade), magnesium sulphate (anhydrous), primary Secondary Amine, Sodium Chloride (AR grade), Sodium Sulphate (anhydrous), Calcium Chloride anhydrous (AR grade), Florosil -chromatography grade) were either of HPLC / LC-MS/MS or AR grade was used for residue analysis. Sodium sulphate, sodium chloride and magnesium sulphate were activated prior to use. All equipment and instruments were calibrated to meet performance criteria. Commercially available granular form of fipronil (Regent 0.3G) formulation marketed by Bayer crop science, India was used for soil application in the experimental plot.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!