Human SCLC cell lines (DMS53, NCI-H209, NCI-H69, SHP77, NCI-H889, NCI-H345, and NCI-H82) and human NSCLC cell lines (A549, NCI-H23, NCI-H460, NCI-H1155, NCI-H358, NCI-H727, NCI-H1770, and NCI-H125) were maintained in RPMI 1640 (Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum, and 1% penicillin streptomycin, as previously described (Hong et al. 2013 (link)). All SCLC and the two NSCLC lines, NCI-H1155 and NCI-H727, were previously characterized as NE tumors (Carney et al. 1985 (link); Gazdar and Minna 1996 (link); Linnoila 1996 (link)). Human primary diploid fibroblasts IMR90 were maintained in MEM (Invitrogen, Carlsbad, CA) supplemented with 10% bovine growth serum, 1% sodium pyruvate, 1% MEM non-essential amino acids, and 1% penicillin streptomycin. Vandetanib was purchased from Invitrogen and LC Laboratories (Woburn, MA). Cabozantinib was purchased from Selleckchem (Huston, TX).
Vandetanib
Manufactured by Thermo Fisher Scientific
Sourced in United States
Vandetanib is a small-molecule tyrosine kinase inhibitor that targets the vascular endothelial growth factor receptor (VEGFR), epidermal growth factor receptor (EGFR), and rearranged during transfection (RET) protein kinases. It is primarily used in research applications to study the role of these signaling pathways in various cellular processes and disease models.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640, by Thermo Fisher Scientific (2 mentions)
Cabozantinib, by Selleck Chemicals (1 mentions)
Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions)
Mkt 077, by Merck Group (1 mentions)
5 and 6 carboxy 2 7 dichlorodihydrofluorescein diacetate carboxy h2 dcfda, by Thermo Fisher Scientific (1 mentions)
Tetramethylrhodamine ethyl ester perchlorate, by Merck Group (1 mentions)
High glucose dmem, by Thermo Fisher Scientific (1 mentions)
N acetylcysteine, by Merck Group (1 mentions)
2 protocols using vandetanib
1
Culturing SCLC and NSCLC Cell Lines
2
Cell Line Maintenance and Reagent Protocols
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The human MTC lines, TT and MZ-CRC-1, were maintained as previously described [19 (link)20 (link)21 (link)]. Briefly, TT was maintained in RPMI 1640 (Invitrogen, Carlsbad, CA, USA) supplemented with 16% fetal bovine serum (FBS), 100 units of penicillin and 100 µg of streptomycin per mL. MZ-CRC-1 was maintained in high-glucose DMEM (Invitrogen) supplemented with 10% FBS in culture dishes coated with rat collagen (Sigma, St. Louis, MO, USA). All experiments were performed using cells within 10 passages from the point of acquisition. MKT-077, tetra-methyl-rhodamine ethyl ester perchlorate (TMRE), and N-acetyl-cysteine (NAC) were purchased from Sigma. 5-(and-6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA) and vandetanib were purchased from Invitrogen and LC Laboratories (Woburn, MA, USA), respectively.
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