Bone marrow mesenchymal stem cells were identified by flow cytometry assay. The cells were washed with PBS. The cells were then resuspended in the PBS‐2%BSA. The cells were adjusted to 2 × 107 cells/mL. Then we added the primary antibody including CD29 Monoclonal Antibody (eBioscience, 11–0291‐82, USA), CD29 Monoclonal Antibody (eBioscience, 11–0461‐82, USA), and CD90 Monoclonal Antibody (eBioscience, MA1‐80648, USA) at the recommended concentration according to the manufacturer's instructions. After incubation and centrifugation, flow cytometry assay (Becton Dickinson, USA) was performed.
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