Real time rt pcr reagents

Manufactured by Thermo Fisher Scientific

Real-time RT-PCR reagents are a set of laboratory products used for the detection and quantification of RNA molecules through the process of reverse transcription and real-time polymerase chain reaction (RT-PCR). These reagents include enzymes, buffers, primers, and probes necessary to perform this analytical technique.

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Lab products found in correlation

Bovine serum albumin (bsa), by Merck Group (2 mentions) Sucrose, by Merck Group (2 mentions) Nadph, by Merck Group (2 mentions) Goat anti mouse igg conjugated with horseradish peroxidase, by Bio-Rad (2 mentions) Ethoxyresorufin, by Merck Group (2 mentions) Ethylene diamine tetraacetic acid (edta), by Merck Group (1 mentions) Methoxyresorufin, by Merck Group (1 mentions)

Spelling variants of this product

Real-time PCR reagents (15 citations) RT-PCR reagents (13 citations) Reagents for real time RT-PCR and Western blotting (2 citations)

2 protocols using real time rt pcr reagents

Enzyme Activity Quantification Assay

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Tris, sucrose, NADPH, bovine serum albumin, ethoxyresorufin, methoxyresourufin, EDTA, and butylated hydroxytoluene were purchased from Sigma Chemical Co. (St. Louis, MO). Buffer components for electrophoresis and Western blotting and Goat anti-mouse IgG conjugated with horseradish peroxidase were obtained from Bio-Rad laboratories (Hercules, CA). Rat anti Mouse Ly-6B.2 monoclonal antibody, clone 7/4 (Cat no: MCA771G) was purchased from AbD Serotec (Bio-Rad laboratories, Richmond CA), the primary monoclonal antibody to CYP1A1 was obtained from Dr. P.E. Thomas, Rutgers University. All real-time RT-PCR reagents were bought from Applied Biosystems (Foster City, CA).

Wang L., Lingappan K., Jiang W., Couroucli X.I., Welty S.E., Shivanna B., Barrios R., Wang G., Khan M.F., Gonzalez F.J., Roberts L.J, & Moorthy B. (2015). Disruption of Cytochrome P4501A2 in mice leads to increased susceptibility to hyperoxic lung injury. Free radical biology & medicine, 82, 147-159.

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Quantitative Analysis of CYP1A1/1A2 Proteins

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Tris, sucrose, NADPH, bovine serum albumin, ethoxyresorufin, methoxyresorufin were purchased from Sigma Chemical Co. (St. Louis, MO). Buffer components for electrophoresis and Western blotting were obtained from Bio-Rad laboratories (Hercules, CA). The primary monoclonal antibody to CYP1A1, which cross-reacts with CYP1A2, was obtained from Dr. P. E. Thomas (Rutgers University, Piscataway, NJ). Goat anti-mouse IgG conjugated with horseradish peroxidase was from Bio-Rad laboratories (Richmond, CA). All Real Time RT-PCR reagents were from Applied Biosystems (Foster City, CA).

Lingappan K., Jiang W., Wang L., Couroucli X.I, & Moorthy B. (2015). Sex-specific differences in hyperoxic lung injury in mice: role of Cytochrome P450 (CYP)1A. Toxicology, 331, 14-23.

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