Keratinocytes were seeded on 8-chamber polystyrene slides (Corning Incorporated, NY 14831, USA) and were differentiated using 1.2 mM CaCl2 overnight. Active caspase-1 was visualized using FAM FLICA Caspase-1 Kit (Bio-Rad Laboratories GmbH, D-85622 Feldkirchen, Germany). Hoechst was performed using 0.5% Hoechst reagent followed by propidium iodide (PI) staining (0.5% PI). Then, cells were fixed using fixative solution diluted 1 : 10 in apoptosis wash buffer. Pictures were taken using a Zeiss LSM 800 confocal laser scanning microscope with Zeiss ZEN 2.3 (blue edition) Software (Carl Zeiss Microscopy GmbH, Jena, Germany).
8 chamber polystyrene slides
Manufactured by Corning
Sourced in United States
The 8-chamber polystyrene slides are a type of laboratory equipment designed for cell culture applications. These slides feature a multi-well format, providing eight individual chambers within a single unit. Polystyrene, a common material used in laboratory consumables, is the primary composition of these slides. The core function of these 8-chamber slides is to provide a platform for culturing and studying cells in a controlled and organized manner.
Automatically generated - may contain errors
Lab products found in correlation
Lsm 800 confocal laser scanning microscope, by Zeiss (1 mentions)
Zen 2.3 blue edition software, by Zeiss (1 mentions)
Fam flica caspase 1 kit, by Bio-Rad (1 mentions)
Lipofectamine rnaimax reagent, by Thermo Fisher Scientific (1 mentions)
Opti mem 1x reduced serum media, by Thermo Fisher Scientific (1 mentions)
2 protocols using 8 chamber polystyrene slides
1
Visualizing Caspase-1 Activation in Differentiated Keratinocytes
2
Transfecting Cells with Poly(dA:dT) DNA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were seeded on 8-chamber polystyrene slides (Corning Incorporated, NY 14831, USA) (for ASC staining), until they reached 60-70% confluency. Then, cells were transfected in the Opti-MEM (1x) reduced serum media (Gibco, Thermo Fisher Scientific, Karlsruhe, Germany), with 5 μg/ml poly(dA:dT) naked double-stranded B-DNA (InvivoGen, San Diego, USA) for 24 h, using Lipofectamine RNAiMax reagent (Thermo Fisher Scientific, Karlsruhe, Germany). Opti-MEM media were used as a negative control.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!