For histological analysis, the left inguinal mammary gland was harvested and fixed in 4% Paraformaldehyde overnight prior to paraffin embedding. For conventional histological analysis, mammary gland tissue slides were stained with Hematoxylin and Eosin (H&E). For ductal quantification, mammary gland H&E histological images were uploaded into Fiji (Fiji, RRID:SCR_002285), and ducts present in the posterior part of the gland were manually counted. Immunohistochemistry staining (IHC) was performed on a Roche Discovery Ultra Automated IHC/ISH stainer. For Masson’s trichrome staining, Leica Multistainer Stainer/Coverslipper Combo (ST5020-CV5030) was used to stain slides according to standard reagents and protocols. Images were acquired using Aperio ePathology (Leica Biosystems) slide scanner in 40X lenses.
Discovery ultra automated ihc ish stainer
Manufactured by Roche
The Discovery Ultra Automated IHC/ISH Stainer is a laboratory equipment designed for immunohistochemistry (IHC) and in-situ hybridization (ISH) staining applications. It provides automated processing of tissue samples to enable standardized and reproducible staining results.
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2 protocols using discovery ultra automated ihc ish stainer
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Histological Analysis of Mammary Gland
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Histological Analysis of Mammary Gland
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For histological analysis, the left inguinal mammary gland was harvested and fixed in 4% Paraformaldehyde overnight prior to paraffin embedding. For conventional histological analysis, mammary gland tissue slides were stained with Hematoxylin and Eosin (H&E). For ductal quantification, mammary gland H&E histological images were uploaded into Fiji (Fiji, RRID:SCR_002285), and ducts present in the posterior part of the gland were manually counted. Immunohistochemistry staining (IHC) was performed on a Roche Discovery Ultra Automated IHC/ISH stainer. For Masson’s trichrome staining, Leica Multistainer Stainer/Coverslipper Combo (ST5020-CV5030) was used to stain slides according to standard reagents and protocols. Images were acquired using Aperio ePathology (Leica Biosystems) slide scanner in 40X lenses.
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