Nanodrop uv vis spectrophotometer q5000

In compliance with the manufacturer's instructions, TRIzol reagent was used to extract total RNA using the (Life Technologies, Gaithersburg, MD, USA). The purity and concentration of the RNA extracted were verified using a Nanodrop (UV-Vis spectrophotometer Q5000/Quawell, USA). After that, the MultiScribe kits of RT enzyme (Applied Biosystems,USA) were utilized to synthesize cDNA. The analysis of triplicate real-time PCR was performed on the resultant cDNA using a System of 7500 Real-Time PCR (Foster City, California, USA: Applied Biosystems) and Master Mix for Power SYBR Green PCR (Applied Biosystems, USA). The variation in fold in the mRNA levels of the measured genes was normalized using the expression of GAPDH, a typical housekeeping gene. Different genes' mRNA expression fold change was evaluated compared to the control. Table 2 contains the gene accession codes and primer sequences. Thus, the 2 -∆∆Ct approach was used to equalize the target gene's critical threshold (Ct) quantities with the gene of housekeeping amounts (Ct) [34] (link).