Quenched samples were centrifuged at 3000 g for 10 minutes at 4°C and supernatants were collected to new tubes. Samples were evaporated for 2 hours until <200 µL remained. These were diluted to 1 mL with 0.05% formic acid (FA) in Milli-Q water and extracted using solid-phase extraction (SPE). The samples were loaded onto Oasis HLB 1cc 30 mg cartridges (Waters Corporation, Milford, MA, USA) that had been preconditioned with 100% MeOH and 0.05% FA in Milli-Q. SPE columns were washed once with 10% MeOH in Milli-Q containing 0.05% FA followed by elution in 1 mL of 100% MeOH. The samples were evaporated to dryness under vacuum, and stored at -20°C until analyzed with liquid chromatography (LC)-tandem mass spectrometry (MS/MS). To be able to compare the results from different experiments the levels of individual prostaglandins in each sample were normalized to the sample's total prostaglandin production (Figure S3).
Oasis hlb 1cc 30 mg cartridges
Manufactured by Waters Corporation
Sourced in United States
The Oasis HLB 1cc 30 mg cartridges are solid-phase extraction (SPE) products designed for sample preparation. These cartridges contain a hydrophilic-lipophilic balanced (HLB) sorbent material, which can be used to extract a wide range of analytes from various sample matrices.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using oasis hlb 1cc 30 mg cartridges
1
Quantitative Prostaglandin Profiling
2
Menbutone Quantification in Sheep
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Menbutone pure reference standard (98.99%) was purchased from LGC-Dr. Ehrenstorfer (LGC Labor GmBH, Ausburg, Germany). Sparfloxacin was used as internal standard (IS) (purity 98%), and supplied from Sigma-Aldrich (Merck, Darmstadt, Germany). Reagents and solvents used for drug extraction and analysis were: Acetonitrile (HiPerSolv Chromanorm®, VWR, Radnor, PA, USA), methanol (LiChrosolv, Merck, Madrid, Spain), monopotassium phosphate (AnalaR Normapur®, Radnor, PA, USA), sodium hydroxide 1N (Panreac Quimica S.A., Barcelona, Spain) and acetic acid (HiPerSolv Chromanorm, VWR, Radnor, PA, USA). HPLC grade water was produced in our laboratory by using a Millipore Milli-Q Gradient water purification system (Waters Corporation, Mildford, MA, USA), and used for the extraction and quantification procedures. For the solid phase extraction (SPE) Oasis HLB 1cc 30 mg cartridges (Waters Corporation, Mildford, MA, USA) were employed. A commercially available formulation (Digestosyva® 100 mg/mL, Laboratorios Syva S.A.U., Leon, Spain) containing menbutone was used for IV and IM administrations to sheep.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!