Novel ICSI-AOA test was an experimental test to explore effective methods to rescue fertilization failure. For paternal-factor-induced fertilization failure, injected oocytes were acquired from donated immature oocytes and in vitro mature oocytes from other IVF individuals. For maternal-factor-induced fertilization failure, oocytes were donated by the female patient with a written consent to research. Since ionomycin was reported to be a more specific Ca2+ ionophore with higher reported fertilization rates than A23187, in the novel ICSI-AOA test procedure, oocytes after ICSI were immediately transferred to the ionomycin (5 μM, MB7511, Meilunbio, Dalian, China) medium for 5 min of incubation before another 6 h interval in the CHX (10 μg/ml, a gift from Chinese Academy of Sciences) medium which was pre-equilibrated for at least 6 h. After AOA, oocytes were then transferred into the G1-plus medium (Vitrolife, Gothenburg, Sweden) for future culture after washing.
Ionomycin
Manufactured by Meilun
Sourced in China
Ionomycin is a laboratory reagent used as a calcium ionophore. It facilitates the movement of calcium ions across cell membranes, which can be used to study calcium-dependent cellular processes.
Automatically generated - may contain errors
2 protocols using ionomycin
1
Novel ICSI-AOA Fertilization Rescue Protocol
2
Inhibiting Platelet Aggregation and Metastasis
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For inhibiting platelet aggregation, aspirin (catalog no. J20171021, Bayer) at 30 mg/kg or warfarin (catalog no. H31022123, SINE) at 30 mg/kg was given orally once per day to each mouse for 10 days. Distilled water was used as a control. For metastasis inhibition, a selective GRP75 inhibitor MKT-077 (catalog no. A12388, AdooQ Bioscience) at 15 mg/kg was intraperitoneally injected once per day into each mouse. DMSO (catalog no. MB2505, Meilunbio) was used as a control. For in vitro platelet activation, freshly isolated mouse or human platelets were treated with ADP (catalog no. MB1706, Meilunbio), collagen (catalog no. MB5213, Meilunbio), thrombin (catalog no. MB1368, Meilunbio), ionomycin (catalog no. MB7511, Meilunbio), and arachidonic acid (AA, catalog no. MB6012, Meilunbio). In some experiments, a GRP75 inhibitor MKT-077 (catalog no. A12388, AdooQ Bioscience) at 20 mmol/L was pretreated for 30 minutes prior to agonist stimulation.
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