Blood was collected into pre-chilled tubes, centrifuged at 1,000 g, and plasma was snap-frozen and stored at −80 °C until the analysis. Plasma glucose (ABX Pentra Glucose HK CP, Horiba ABX Diagnostics), FFA (WAKO NEFA-HR [2 ] ACS-ACOD method, WAKO Chemicals GmbH), TAG (ABX Pentra Triglycerides CP, Horiba ABX Diagnostics), and glycerol (Glycerol-kit UV-test, r-Biopharm) were determined. Plasma insulin was measured using a double-antibody radioimmunoassay (Millipore). Lactate was measured in plasma using standard enzymatic techniques automated on a Cobas Fara centrifugal spectrophotometer (Roche Diagnostics). Plasma TNFα, IFNγ, IL-6, and IL-8 concentrations were determined in fasting plasma samples collected at day 8, subsequent to 7-d MIH exposure, using the V-PLEX Pro-inflammatory Panel I Human Kit (Mesoscale, 4-plex, no. K15052D), according to manufacturer's guidelines.
Abx pentra glucose hk cp
Manufactured by Horiba
Sourced in France
The ABX Pentra Glucose HK CP is a laboratory equipment designed for the analysis of glucose levels. It utilizes the hexokinase method to measure glucose concentration in various sample types.
Automatically generated - may contain errors
Lab products found in correlation
V plex proinflammatory panel 1 human kit, by Mesoscale (1 mentions)
Double antibody radioimmunoassay, by Merck Group (1 mentions)
Abx pentra triglycerides cp, by Horiba (1 mentions)
Human insulin kit, by Mesoscale (1 mentions)
Acquity uplc h class with qda, by Waters Corporation (1 mentions)
Abx pentra 400, by Horiba (1 mentions)
Immulite 1000 immunoassay system, by Siemens (1 mentions)
3 protocols using abx pentra glucose hk cp
1
Plasma Biomarker Analysis in MIH Exposure
2
Plasma Biomarker Quantification Protocol
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Blood samples were collected in tubes containing EDTA and centrifuged at 1,000g for 10 min at 4 °C. Aliquots of plasma were frozen in liquid nitrogen and stored at -80 °C. Plasma glucose and insulin concentrations were analyzed using commercially available kits (ABX Pentra Glucose HK CP, Horiba ABX, ref:
A11A01667 and Human Insulin kit, Meso Scale Discovery, ref: K151BZC). Quantification of plasma AA concentrations was performed using ultra-performance liquid chromatography mass spectrometry (ACQUITY UPLC H-Class with QDa; Waters), as described previously (Nyakayiru et al., 2019) (link).
A11A01667 and Human Insulin kit, Meso Scale Discovery, ref: K151BZC). Quantification of plasma AA concentrations was performed using ultra-performance liquid chromatography mass spectrometry (ACQUITY UPLC H-Class with QDa; Waters), as described previously (Nyakayiru et al., 2019) (link).
3
Glucose and insulin measurement protocol
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The blood samples were collected in dry tubes for the measurement of insulin and in fluoride citrate tubes for the measurement of glucose. Plasma glucose was measured with an enzymatic colorimetric method (ABX Pentra Glucose HK CP; Horiba, Montpellier, France) and analyzed with an ABX Pentra 400 (Horiba). Serum insulin was measured by a chemiluminescent immunometric assay with an Immulite 1000 Immunoassay System (Siemens Healthcare Diagnostics, West Sacramento, CA). All samples were analysed in one batch. Intra-assay and interassay coefficients of variation (CVs) for glucose and insulin were below 2.8%.
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