The ZFL cell line was purchased from American Type Culture Collection (Manassas, VA, USA), and cultured according to established protocols (24 (link), 36 (link)). All media were obtained from Corning, Inc. Penicillin–Streptomycin solution and bovine insulin were purchased from Sigma (St. Louis, MO, USA). Murine epidermal growth factor was purchased from Peprotech (Rocky Hill, NJ, USA). Rainbow trout (Oncorhynchus mykiss) serum was purchased from Caisson Labs (USA).
Rainbow trout oncorhynchus mykiss serum
Manufactured by Caisson
Sourced in United States
Rainbow trout Oncorhynchus mykiss serum is a laboratory product derived from the blood serum of the rainbow trout species. It can be used for various research and analytical applications involving this specific fish species.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Hepes, by Merck Group (4 mentions)
Dmem f12, by Thermo Fisher Scientific (4 mentions)
Penicillin, by Merck Group (4 mentions)
Streptomycin, by Merck Group (4 mentions)
Trizol reagent, by Thermo Fisher Scientific (2 mentions)
Murine epidermal growth factor, by Thermo Fisher Scientific (1 mentions)
Bovine insulin, by Merck Group (1 mentions)
Penicillin streptomycin solution, by Merck Group (1 mentions)
Zfl cell line, by American Type Culture Collection (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
5 protocols using rainbow trout oncorhynchus mykiss serum
1
Culturing ZFL Cell Line
2
Culturing Rabbitfish and Human Kidney Cells
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The rabbitfish S. canaliculatus hepatocyte line (SCHL) was previously established in our laboratory (Liu et al. 2017) . Cells were cultured at 28 ℃ in Dulbecco's modified Eagle's medium/nutrient F12 (DMEM/F12, Gibco, Life Technologies, USA) containing 20 mM 4-(2-hydroxyethyl) piperazine-1-ethanesulphonic acid (HEPES, Sigma-Aldrich, USA), 10 % fetal bovine serum (FBS, Gibco, Life Technologies, USA), 0.5 % rainbow trout Oncorhynchus mykiss serum (Caisson Labs), penicillin (100 U ml - 1 , Sigma-Aldrich, USA) and streptomycin (100 U ml -1 , Sigma-Aldrich, USA). Human embryonic kidney cells (HEK 293T, Chinese Type Culture Collection, Shanghai, China) were grown in High Glucose Dulbecco's Modified Eagle Medium (DMEM, Gibco, Life Technologies, USA) supplemented with 10 % FBS (Sijiqing Biological Engineering Material Company, China) and maintained at 37 ℃ with 5 % CO2.
3
Rabbitfish Hepatocyte Cell Culture
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The rabbitfish S. canaliculatus hepatocyte line (SCHL) was successfully established previously (Liu et al., 2017) . Before the experiment, SCHL cells were cultured at 28 °C in Dulbecco's modified Eagle's medium/nutrient F12 (DMEM/F12, Gibco, Life Technologies, USA) containing 20 mM 4-(2-hydroxyethyl) piperazine-1-ethanesulphonic acid (HEPES, Sigma-Aldrich, USA), 10 % fetal bovine serum (FBS, Gibco), 0.5 % rainbow trout (Oncorhynchus mykiss) serum (Caisson Labs), penicillin (100 U ml-1, Sigma-Aldrich), and streptomycin (100 U ml-1, Sigma-Aldrich).
Fatty acid/ BSA complexes of PUFA including LA, ALA, ARA, EPA and DHA (Cayman, Ann Arbor, USA) at 10 mM concentration were prepared according to Ou et al. (2001) and stored at -20 °C. The SCHL cells were seeded into six-well plates at a density of 1.0 × 106 cells per well in DMEM/F12 supplemented with 5 % FBS and 0.1 % rainbow trout serum. After 24 h, cells were incubated for 2 h in serum-free DMEM/F12 and then exposed to fresh DMEM/F12 medium containing LA, ALA, ARA, EPA or DHA at 100μM in triplicate per treatment. In addition, 0.1% BSA was used as control for PUFA treatments. After incubation for 24 h, the cells were lysed with Trizol reagent (Invitrogen) for total RNA isolation.
Fatty acid/ BSA complexes of PUFA including LA, ALA, ARA, EPA and DHA (Cayman, Ann Arbor, USA) at 10 mM concentration were prepared according to Ou et al. (2001) and stored at -20 °C. The SCHL cells were seeded into six-well plates at a density of 1.0 × 106 cells per well in DMEM/F12 supplemented with 5 % FBS and 0.1 % rainbow trout serum. After 24 h, cells were incubated for 2 h in serum-free DMEM/F12 and then exposed to fresh DMEM/F12 medium containing LA, ALA, ARA, EPA or DHA at 100μM in triplicate per treatment. In addition, 0.1% BSA was used as control for PUFA treatments. After incubation for 24 h, the cells were lysed with Trizol reagent (Invitrogen) for total RNA isolation.
4
Rabbitfish Hepatocyte Cell Culture
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The rabbitfish S. canaliculatus hepatocyte line (SCHL) was successfully established previously (Liu et al., 2017) . Before the experiment, SCHL cells were cultured at 28 °C in Dulbecco's modified Eagle's medium/nutrient F12 (DMEM/F12, Gibco, Life Technologies, USA) containing 20 mM 4-(2-hydroxyethyl) piperazine-1-ethanesulphonic acid (HEPES, Sigma-Aldrich, USA), 10 % fetal bovine serum (FBS, Gibco), 0.5 % rainbow trout (Oncorhynchus mykiss) serum (Caisson Labs), penicillin (100 U ml-1, Sigma-Aldrich), and streptomycin (100 U ml-1, Sigma-Aldrich).
Fatty acid/ BSA complexes of PUFA including LA, ALA, ARA, EPA and DHA (Cayman, Ann Arbor, USA) at 10 mM concentration were prepared according to Ou et al. (2001) and stored at -20 °C. The SCHL cells were seeded into six-well plates at a density of 1.0 × 106 cells per well in DMEM/F12 supplemented with 5 % FBS and 0.1 % rainbow trout serum. After 24 h, cells were incubated for 2 h in serum-free DMEM/F12 and then exposed to fresh DMEM/F12 medium containing LA, ALA, ARA, EPA or DHA at 100μM in triplicate per treatment. In addition, 0.1% BSA was used as control for PUFA treatments. After incubation for 24 h, the cells were lysed with Trizol reagent (Invitrogen) for total RNA isolation.
Fatty acid/ BSA complexes of PUFA including LA, ALA, ARA, EPA and DHA (Cayman, Ann Arbor, USA) at 10 mM concentration were prepared according to Ou et al. (2001) and stored at -20 °C. The SCHL cells were seeded into six-well plates at a density of 1.0 × 106 cells per well in DMEM/F12 supplemented with 5 % FBS and 0.1 % rainbow trout serum. After 24 h, cells were incubated for 2 h in serum-free DMEM/F12 and then exposed to fresh DMEM/F12 medium containing LA, ALA, ARA, EPA or DHA at 100μM in triplicate per treatment. In addition, 0.1% BSA was used as control for PUFA treatments. After incubation for 24 h, the cells were lysed with Trizol reagent (Invitrogen) for total RNA isolation.
5
Establishment of Rabbitfish Hepatocyte Line
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The rabbitfish S. canaliculatus hepatocyte line (SCHL) was successfully established in our laboratory (Liu et al. 2017 ) SCHL cell line was cultured at 28 ºC in Dulbecco's modified Eagle's medium/nutrient F12 (DMEM/F12, Gibco, Life Technologies, USA) containing 20 mM 4-(2-hydroxyethyl) piperazine-1ethanesulphonic acid (HEPES, Sigma-Aldrich, USA), 10 % fetal bovine serum (FBS, Gibco, Life Technologies, USA), 0.5 % rainbow trout Oncorhynchus mykiss serum (Caisson Labs), penicillin (100 U ml -1 , Sigma-Aldrich, USA) and streptomycin (100 U ml -1 , Sigma-Aldrich, USA).
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