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Rna extraction column kit

Manufactured by EZBioscience
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Sourced in United States

The RNA extraction column kit is a laboratory equipment designed for the efficient extraction and purification of RNA from various biological samples. It utilizes a silica-based column that selectively binds RNA, allowing for the removal of impurities and contaminants. The kit provides a simple and reliable method for obtaining high-quality RNA for downstream applications such as gene expression analysis, reverse transcription, and other molecular biology techniques.

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Lab products found in correlation

Cdna synthesis kit, by EZBioscience (2 mentions) Sybr green master mix, by EZBioscience (2 mentions) Lightcycler 480, by Roche (2 mentions)

2 protocols using rna extraction column kit

1

RNA Extraction and qRT-PCR Analysis of BMDMs

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After 24 h of incubation, total RNA was extracted from BMDMs in the four groups using an RNA extraction column kit (EZBioscience, USA) according to the manufacturer’s instructions. Subsequently, complementary DNA was synthesized from 1 μg of total RNA using a cDNA synthesis kit (EZBioscience, USA). Quantitative RT-PCR was performed using a SYBR Green Master mix (EZBioscience, USA) on LightCycler 480 (Roche, USA). The primers used are shown in Additional file 1: Table S1.
Ding C., Yang C., Cheng T., Wang X., Wang Q., He R., Sang S., Zhu K., Xu D., Wang J., Liu X, & Zhang X. (2021). Macrophage-biomimetic porous Se@SiO2 nanocomposites for dual modal immunotherapy against inflammatory osteolysis. Journal of Nanobiotechnology, 19, 382.
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2

RNA Extraction and qRT-PCR Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
After 24 h of incubation, the total RNA was extracted using an RNA extraction column kit (EZBioscience, USA) according to the manufacturer's instructions. Subsequently, complementary DNA was synthesized from 1 μg of total RNA using a cDNA synthesis kit (EZBioscience, USA). Quantitative RT-PCR was performed using a SYBR Green Master mix (EZBioscience, USA) on LightCycler 480 (Roche, USA). The primers used are shown in Table S1.
Ding C., Yang C., Cheng T., Wang S., Wang Q., He R., Sang S., Zhu K., Dong-dong X., Wang J., Liu X., & Zhang X. (2021). Macrophage-Biomimetic Porous Se@SiO2 Nanocomposites For “Dual Model” Immunotherapy Against Inflammatory Osteolysis.
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