Cultured primary urinary cells were collected in cold PBS. Cell lysis was performed by freezing and thawing over five cycles. Enzyme activity for α-Galactosidase A (α-Gal) and β-Galactosidase (β-Gal) was measured after incubation of lysed cells with 4-methylumbelliferyl a-D-galactopyranoside for α-Gal or 4-methylumbelliferyl b-D-galactopyranoside for β-Gal (Sigma, Taufkirchen, Germany) as described previously39 (link) α-Gal A activity was normalized to β-Gal activity, functioning as an internal control.
4 methylumbelliferyl b d galactopyranoside for β gal
Manufactured by Merck Group
Sourced in Germany
4-methylumbelliferyl b-D-galactopyranoside is a fluorogenic substrate used to detect and quantify the enzyme β-Galactosidase (β-Gal) activity. Upon cleavage by β-Gal, the compound releases a fluorescent 4-methylumbelliferone product that can be measured.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using 4 methylumbelliferyl b d galactopyranoside for β gal
1
Measuring α-Galactosidase A and β-Galactosidase Activities
2
Podocyte α-Galactosidase Activity Assay
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Cultured human podocytes were collected in cold PBS. Cell lysis was performed by freezing and thawing over five cycles. Enzyme activity for α-Galactosidase A (α-Gal) and β-Galactosidase (β-Gal) was measured after incubation of lysed cells with 4-methylumbelliferyl a-D-galactopyranoside for α-Gal or 4-methylumbelliferyl b-D-galactopyranoside for β-Gal (Sigma, Taufkirchen, Germany) as described previously [12] . α-Gal A activity was normalized to β-Gal activity, functioning as an internal control.
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