3 5 dcqa

Green coffee (Coffea arabica L.) from Colombia, and yerba mate (Ilex paraguariensis St. Hil.), from Argentina, were purchased in a local supermarket in Madrid (Spain).
Gentamicin, penicillin, streptomycin, non-essential amino acids (NEAA), McCoy's 5A medium, and minimum essential culture medium (MEM) were acquired from Sigma-Aldrich (Madrid, Spain). Fetal bovine serum (FBS), Dulbecco's modified eagle's medium (DMEM)-F12 and RPMI 1640 with glutamine were from Biowhitaker Europe (Lonza, Madrid, Spain). Cell proliferation ELISA 5-bromo-2'-deoxyuridine (BrdU) kit and cell viability reagent WST-1 were purchased from Roche Diagnostics (Barcelona, Spain). Protein assay reagent was from Bio-Rad Laboratories (Madrid, Spain). CA, FA, 5-CQA, DHCA, DHFA, caffeine, and dimethylsulphoxide (DMSO) were from Sigma-Aldrich (Madrid, Spain). 3,5-DCQA was from Phytolab (Vestenbergsgreuth, Germany).
Human lung carcinoma A549, human colorectal adenocarcinoma Caco-2, human oesophageal carcinoma OE-33, human urinary bladder carcinoma T24, non-cancer fibroblastic cell line CCD-18Co were supplied by the Cell Bank of the Scientific Instrumentation Centre at the University of Granada (CIC-UGR, Granada, Spain).

Green coffee (Coffea arabica L. from Colombia) and yerba mate (Ilex paraguariensis L.) were purchased in a local supermarket in Madrid (Spain). 3,5-DCQA was acquired from PhytoLab (Vestenbergsgreuth, Germany). Caffeine was obtained from Fluka (Madrid, Spain). 5-CQA, CA, FA, theobromine, DHCA, DHFA were obtained from Sigma-Aldrich (Madrid, Spain). Phycoerythrin (PE)-conjugated mouse anti-human CD61 (CD61-PE), allophycocyanin (APC)-conjugated mouse anti-human P-selectin (CD62-APC), PE-conjugated mouse IgG1κ, APC-conjugated mouse IgG1κ, AF488-conjugated mouse IgG1κ, sodium chloride (NaCl) and FACS Flow sheath fluid were acquired from BD Biosciences (Oxford, UK). AF488-conjugated fibrinogen from human plasma was obtained from Fisher Scientific (Loughborough, UK). Adenosine 5`-diphosphate (ADP), phosphate-buffered saline (PBS), dimethylsulfoxide (DMSO), potassium chloride (KCl), magnesium sulfate heptahydrate (MgSO 4 -7H 2 O), 4-(2-hydroxyethyl)-1-piperazineethanesulphonic acid (HEPES), phorbol 12-myristate 13-acetate (PMA) and quercetin were acquired from Sigma-Aldrich (Dorset, UK). All other chemicals were of analytical grade.