Albumin dextrose catalase adc

Manufactured by BD

Albumin-dextrose-catalase (ADC) is a laboratory reagent used in cell culture and microbiology applications. It is a mixture of albumin, dextrose, and catalase. The core function of ADC is to provide a source of nutrients and to help maintain the integrity of cells in culture.

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Lab products found in correlation

Middlebrook 7h9, by BD (1 mentions) Middlebrook 7h10 agar, by BD (1 mentions) L lysine monohydrochloride, by Merck Group (1 mentions)

Close spelling variants of this product

Dextrose (46 citations) Albumin-dextrose-catalase (42 citations) Catalase (17 citations) Middlebrook albumin dextrose catalase enrichment (ADC; (3 citations) Acid-albumin-dextrose-catalase (ADC, (2 citations)

2 protocols using albumin dextrose catalase adc

Mycobacterial Culture and Inoculation Protocol

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Mycobacterial cultures were grown in Middlebrook 7H9 broth medium or on Middlebrook agar 7H10 (Becton Dickinson) medium supplemented with albumin-dextrose-catalase (ADC; Difco Laboratories) and containing 0.05% Tween 80 and 20 μg/mL kanamycin. The L-lysine monohydrochloride (Sigma) was dissolved in distilled water and used at a concentration of 40 μg/mL. Mycobacterial suspensions for animal inoculation were prepared in PBS from frozen glycerol stocks previously titrated by plating serial dilutions.

Broset E., Saubi N., Guitart N., Aguilo N., Uranga S., Kilpeläinen A., Eto Y., Hanke T., Gonzalo-Asensio J., Martín C, & Joseph-Munné J. (2019). MTBVAC-Based TB-HIV Vaccine Is Safe, Elicits HIV-T Cell Responses, and Protects against Mycobacterium tuberculosis in Mice. Molecular Therapy. Methods & Clinical Development, 13, 253-264.

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Mycobacterium Strains Preparation

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Mtb H37Rv, HN878, CDC1551, Erdman, W148, AH30, CC13, AI10, and KY strains; Mycobacterium bovis BCG; and M. bovis BCG–green fluorescent protein (GFP) were grown in Middlebrook 7H9 broth (BBL Microbiology Systems) supplemented with albumin-dextrose-catalase (ADC; Difco Laboratories) and 0.05% Tween 80 at 37°C for 5 to 7 days to an OD₆₀₀ (optical density at 600 nm) of 0.4 to 0.5. After this, mycobacterial cells were pelleted, resuspended in fresh 7H9 broth with 20% glycerol, and stored at -80°C. One vial of the stock was thawed to enumerate CFU per milliliter. On the day of infection, the cells were thawed, washed, and sonicated before use. Drug-resistant strains were maintained at the Public Health Research Institute (Newark, NJ).

Cheng C.Y., Gutierrez N.M., Marzuki M.B., Lu X., Foreman T.W., Paleja B., Lee B., Balachander A., Chen J., Tsenova L., Kurepina N., Teng K.W., West K., Mehra S., Zolezzi F., Poidinger M., Kreiswirth B., Kaushal D., Kornfeld H., Newell E.W, & Singhal A. (2017). Host sirtuin 1 regulates mycobacterial immunopathogenesis and represents a therapeutic target against tuberculosis. Science immunology, 2(9), eaaj1789.

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