CEC counts were performed by means of the Circulating Endothelial Cell isolation kit (research use only) in combination with the CellSearch system (Janssen Diagnostics LLC, Raritan, NJ, USA), that allows standardization of the whole process of sample collection, cellular selection, monoclonal antibodies labeling, analysis and enumeration of CEC, as previously described. 20 To be scored as a CEC, a CD146 + cell must have a nucleus (DAPI), express CD105, show the morphology of an intact cell, and be negative for CD45. Overall, CEC were defined as CD146 + /CD105 + /DAPI + / CD45 -cells. Counts were expressed as number of CEC per mL of PB.
Circulating endothelial cell isolation kit
Manufactured by Johnson & Johnson
Sourced in United States
The Circulating Endothelial Cell isolation kit is a laboratory product designed to isolate and enrich circulating endothelial cells from blood samples. The kit utilizes specific antibodies and magnetic beads to capture and separate the target cells from the sample.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using circulating endothelial cell isolation kit
1
Standardized Enumeration of Circulating Endothelial Cells
2
Standardized CEC Enumeration in Blood
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CEC counts were performed, within 48 hours from collection, using the Circulating Endothelial Cell isolation kit (research use only) on the CellSearch system (Janssen Diagnostics LLC, Raritan, NJ, USA), allowing to standardize the process of sample collection, cellular selection, monoclonal antibodies labelling, analysis and enumeration of CEC10 (link). CEC were defined as CD146+/CD105+/DAPI+/CD45− cells and their values expressed per mL of PB.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!