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Oligodnas

Manufactured by Thermo Fisher Scientific
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Sourced in China, United States

OligoDNAs are synthetic DNA sequences used in various laboratory applications. They serve as building blocks for genetic analysis, gene expression studies, and molecular biology research. OligoDNAs can be customized to specific sequences and lengths to meet the requirements of different experiments and protocols.

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Lab products found in correlation

Sybr gold, by Thermo Fisher Scientific (1 mentions) Exonuclease t, by New England Biolabs (1 mentions) T4 rna ligase 2 rnl2, by New England Biolabs (1 mentions) Ribolock rnase inhibitor, by Thermo Fisher Scientific (1 mentions) T4 polynucleotide kinase, by Thermo Fisher Scientific (1 mentions) T4 dna ligase, by Toyobo (1 mentions)

2 protocols using oligodnas

1

RNA and DNA Ligation Protocol

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All oligoRNAs used in this study were synthesized by GenScript (Nanjing, China), and the oligoDNAs were purchased from Invitrogen (Shanghai, China). For ligation experiments, a phosphate was enzymatically introduced to the 5′-position of RNA or DNA by using T4 polynucleotide kinase (Thermo Scientific; Pittsburgh, PA, USA). SYBR Gold and RiboLock RNase Inhibitor were also from Thermo Scientific. T4 RNA ligase 2 (Rnl2) and Exonuclease T were purchased from NEW ENGLAND BioLabs (Beijing, China). All other chemicals were from Sigma-Aldrich (St. Louis, MO, USA).
Cheng K., An R., Cui Y., Zhang Y., Han X., Sui Z., Chen H., Liang X, & Komiyama M. (2019). RNA ligation of very small pseudo nick structures by T4 RNA ligase 2, leading to efficient production of versatile RNA rings. RSC Advances, 9(15), 8620-8627.
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2

CRISPR-Cas9 Plasmid Construction for Reln Targeting

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A pair of oligo DNAs (Invitrogen, Carlsbad, CA, USA) corresponding to Reln guide RNA was hybridized and ligated using T4 DNA ligase (TOYOBO, Osaka, Japan) into linearized pSpCas9(BB)‐2A‐GFP (PX458) plasmid (Addgene plasmid # 48138; http://n2t.net/addgene:48138; RRID:Addgene_48138) digested with BbsI (NEB, Ipswich, MA, USA) as previously described.35, 36, 37 DNA primers are listed in Table S2.
Sawahata M., Mori D., Arioka Y., Kubo H., Kushima I., Kitagawa K., Sobue A., Shishido E., Sekiguchi M., Kodama A., Ikeda R., Aleksic B., Kimura H., Ishizuka K., Nagai T., Kaibuchi K., Nabeshima T., Yamada K, & Ozaki N. (2020). Generation and analysis of novel Reln‐deleted mouse model corresponding to exonic Reln deletion in schizophrenia. Psychiatry and Clinical Neurosciences, 74(5), 318-327.
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