RNA isolation, cDNA generation, and quantitative reverse transcription-PCR (qRT-PCR) were performed as described in Van Aken et al. (2013) (link) using Spectrum RNA Plant extraction kits (Sigma-Aldrich, Sydney, Australia), iScript cDNA synthesis kit (Bio-Rad), and a Roche LC480 Lightcycler using SYBRgreen detection assays. All primers for qRT-PCR are shown in supplementary table 4 , Supplementary Material online. Relative expression values were normalized, with untreated Col-0 samples set as 1. Statistical analyses were performed using Student’s t-test throughout the manuscript, except where indicated.
Spectrum rna plant extraction kits
Manufactured by Merck Group
Sourced in Australia
Spectrum RNA Plant extraction kits are designed for the isolation and purification of high-quality RNA from a variety of plant tissues. The kits utilize a simple and efficient protocol to extract RNA, ensuring reliable results for downstream applications.
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Lab products found in correlation
2 protocols using spectrum rna plant extraction kits
1
Comprehensive RNA Expression Analysis
2
Arabidopsis Seedling RNA Extraction
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Seeds were sown on Petri dishes containing MS media + 2% sucrose, stratified for 2-3 days in the cold room and then incubated in long day growth conditions for 2 (Col-0) or 3 (mitochondrial mutants) weeks. Pools of plants (whole seedlings) were then collected on the same day in the morning and immediately placed in liquid nitrogen for storage and further processing. RNA isolation, cDNA generation and qRT-PCR was performed as described in Van Aken et al. (2013) using Spectrum RNA Plant extraction kits (Sigma-Aldrich, Sydney, Australia), iScript cDNA synthesis kit (BIO-RAD, Sydney, NSW, Australia) and a Roche LC480 lightcycler using SYBRgreen detection assays. All primers for qRT-PCR are shown in Table S10.
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