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Solvent green 3

Manufactured by Merck Group
Sourced in United Kingdom

Solvent Green 3 is a dye used as a colorant in various laboratory applications. It is a green-colored substance that can be used to stain or dye materials for identification or visualization purposes in chemical and biological experiments.

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5 protocols using solvent green 3

1

Solvent Green 3 Dilution Protocol

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The organic phase was prepared by mixing Solvent Green 3 (Sigma–Aldrich) and chloroform (Fischer Chemical) to make a 18.96 mg/mL stock solution. This stock solution was diluted 20X for photos and videos in Fig. 1e-f and diluted to 400x for Fig. 1c-d. Fig. 2 utilizes chloroform without dyes.
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2

Porcine Tissue-Derived PLG Microsphere Fabrication

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Porcine abdominal tissue was obtained (≤12 h post mortem) from a local abattoir from adult pigs that were not specifically slaughtered for this study.
PLG microspheres were prepared in-house using a coacervation method. Two suspending vehicles were selected with differing physicochemical attributes; a low viscosity (25 mPa.s at 20°C) non-aqueous vehicle, medium chain triglycerides (MCT), and a more viscous (122 mPa.s at 20°C) aqueous solution comprising 3% w/v carboxymethylcellulose sodium (CMC-Na) in phosphate-buffered saline containing 0.1% w/v polysorbate 20. MCT was obtained from Croda International (Goole, UK). CMC-Na, and colorimetric dyes (Patent Blue V and Solvent Green 3) were obtained from Sigma Aldrich (Gillingham, UK).
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3

Dye-Tinted Droplet Microfluidics

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Droplets were created with deionized (DI) water (Type II, Harleco; Fisher
Scientific, Hampton, NH) tinted with either yellow or green dye (Spice Supreme;
Gel Spice Company, Bayonne, NJ) at a concentration of 10% or 1% (v/v),
respectively. The carrier fluids were: toluene (Fisher Scientific, Figure 1) or 1-pentanol (Acros Organics,
Thermo Fisher Scientific, Waltham, MA, Figures
1
, 2, 3, 4, and 5). All carrier fluids solvents were tinted
with Solvent Green 3 (Sigma-Aldrich, St. Louis, MO) at a concentration of 0.50
mg/mL.
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4

Nonanol Coloring for Microfluidic Visualization

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Nonanol was used for the study as it is a low-volatility solvent (boiling point 213 °C), mitigating evaporation. The physical properties of nonanol are indicated in Table 2.36 (link),37 (link) Nonanol has been colored with Solvent Green 3 from Sigma-Aldrich at concentrations of 1.43 mg/mL. The volume of nonanol added to the inlet channel was ~ 1.2 mL. Additional nonanol was added, once fluid reached third branch in the bifurcation device. This is to maintain a flat meniscus in the inlet.
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5

Synthesis and Characterization of Polyurethane Composites

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Glycerol, sebacic acid, PEG (with the number average molecular weight of 950 -1050 g mol -1 ), HDI, tin(II) 2-ethylhexanoate catalyst (tin(II)), methylene blue (MB), methyl orange (MO), Solvent Green 3 (SG), phosphate buffered saline (PBS) tablets, lipase from porcine pancreas (54 U mg -1 ), Dulbecco's Modified Eagle Medium with high glucose (DMEM), resazurin sodium salt were purchased from Sigma-Aldrich. An organoclay based on inorganic montmorillonite (MMT) treated with an organophilic surface modifier of methyl-tallowbis(hydroxyethyl)-ammonium (TN + CH3(CH2CH2OH)2, where T is tallow with ~65% C18; ~30% C16; ~5% C14 of alkyl chains, Figure S1), Cloisite 30B ® , was provided by Southern Clay Products and dried in a vacuum oven at 80 °C for 1 day prior to use. Tetrahydrofuran (THF) and ethanol was purchased from Thermo Fisher Scientific. Water used in this study was prepared by double distillation.
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