Dulbecco s cell culture medium

Acetonitrile, formic acid 98%, methanol, and ethanol were obtained from Tedia (Fairfield, OH, USA). The ultrapure water was produced using Milli-Q Gradient 10A System (Merck Millipore Corporation, Burlington, MA, USA). The delphinidin-3,5-diglucoside chloride standard was bought from ChromaDex TM (Los Angeles, CA, USA). Cyanidin 3-glucoside was purchased from Indofine Chemical Co. (Somerville, NJ, USA). DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2-azinobis(3-ethylbenzo-thiazoline-6-sulfonic acid)) and Trolox (6-Hidroxi-2,5,7,8-tetrametilchroman-2-carboxylic acid) were acquired from Sigma-Aldrich (St Louis, MO, USA). AAPH (2,2′-azobis ((2-methylpropionamidine) dihydrochloride); Fluorescein; Dulbecco’s cell culture medium and bovine serum albumin were purchased from Sigma. Serum fetal bovine and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) were acquired from Laborclin (Campinas, Brazil). All the chemicals had an analytical and HPLC grade.

Dulbecco’s cell culture medium, bovine serum albumin and 3,4-DHPAA, p-CoA, VA and FA were purchased from Sigma-Aldrich Chemical Company (St. Louis, MO, USA). Fetal bovine serum was purchased from Laborclin (Campinas, São Paulo, Brazil). Tissue culture flasks and cell scrapers were purchased from Nunc (Roskilde, Denmark). All the chemicals were of analytical grade.

All chemicals used in this work were of analytical grade. The water was distilled and deionized using a Millipore water purification system. Bovine lactoferrin was purchased from Life Extension (Florida, USA). The nitrilotriacetic acid, ferric nitrate, and Dulbecco's cell culture medium was obtained from Sigma-Aldrich Chemical Company (St. Louis, MO). Fetal bovine serum was purchased from Gibco™, Thermo Fisher Scientific (Waltham, Massachusetts, EUA). Tissue culture flasks were purchased from Greiner Bio-One International GmbH (Kremsmünster, Austria).