Fvb nhsd

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The FvB/NHsD is a lab equipment product. It serves as a core function for laboratory applications.

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Lab products found in correlation

C57bl 6j, by Charles River Laboratories (3 mentions) Sealsafe 1145t, by Tecniplast (3 mentions) Fvb nhsd males, by Inotiv (2 mentions) Balb c annhsd, by Inotiv (1 mentions) Dba 2j, by Jackson ImmunoResearch (1 mentions) Fvb ncrl, by Charles River Laboratories (1 mentions) Dba 2ncrl, by Charles River Laboratories (1 mentions) Dba 2ntac, by Taconic Biosciences (1 mentions) Fvb ntac, by Taconic Biosciences (1 mentions) Balb canncrl, by Charles River Laboratories (1 mentions) Balb cbyj, by Jackson ImmunoResearch (1 mentions) Fvb nj, by Jackson ImmunoResearch (1 mentions) Balb cj, by Jackson ImmunoResearch (1 mentions)

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FvB/NHsD males

4 protocols using fvb nhsd

Neuronal Culture Generation and In Utero Electroporation

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For the neuronal cultures, FvB/NHsD females were crossed with FvB/NHsD males (both ordered at 8‐ to 10‐weeks old from Envigo). For the in utero electroporation, female FvB/NHsD (Envigo) were crossed with male C57Bl6/J (ordered at 8‐to 10‐weeks old from Charles River). All mice were kept group‐housed in IVC cages (Sealsafe 1145T; Tecniplast) with bedding material (Lignocel BK 8/15 from Rettenmayer) on a 12‐h light/dark cycle at 21°C ± 1°C, humidity at 40%–70% and with food pellets (801727CRM(P) from Special Dietary Service) and water available ad libitum. All animal experiments were conducted in accordance with the European Commission Council Directive 2010/63/EU (CCD approval AVD101002017893).

van Woerden G.M., Bos M., de Konink C., Distel B., Avagliano Trezza R., Shur N.E., Barañano K., Mahida S., Chassevent A., Schreiber A., Erwin A.L., Gripp K.W., Rehman F., Brulleman S., McCormack R., de Geus G., Kalsner L., Sorlin A., Bruel A., Koolen D.A., Gabriel M.K., Rossi M., Fitzpatrick D.R., Wilkie A.O., Calpena E., Johnson D., Brooks A., van Slegtenhorst M., Fleischer J., Groepper D., Lindstrom K., Innes A.M., Goodwin A., Humberson J., Noyes A., Langley K.G., Telegrafi A., Blevins A., Hoffman J., Guillen Sacoto M.J., Juusola J., Monaghan K.G., Punj S., Simon M., Pfundt R., Elgersma Y, & Kleefstra T. (2021). TAOK1 is associated with neurodevelopmental disorder and essential for neuronal maturation and cortical development. Human Mutation, 42(4), 445-459.

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Inbred Mouse Substrain Phenotypes

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Information on all inbred substrains including source and cage environment (cohoused vs not cohoused) is provided in Supplementary Table 1. A/J, BALB/c, FVB/N and DBA/2 substrains were purchased from their respective commercial vendors and housed in substrain specific cages throughout testing. Mice were an average of 27 days old upon arrival to UNC and were acclimated to the vivarium for 5 weeks after arrival before behavioral testing. A/J substrains were A/J (The Jackson Laboratory, 000646), A/JCr (Charles River Laboratories, 563) and A/JOlaHsd (Envigo, 049). BALB/c substrains were BALB/cJ (The Jackson Laboratory, 000651), BALB/cByJ (The Jackson Laboratory, 001026), BALB/cAnNCrl (Charles River Laboratories, 028) and BALB/cAnNHsd (Envigo, 047). FVB/N substrains were FVB/NJ (The Jackson Laboratory, 001800), FVB/NCrl (Charles River Laboratories, 207), FVB/NHsd (Envigo, 118), and FVB/NTac (Taconic Biosciences, FVB-F/FVB-M). DBA/2 substrains were DBA/2J (The Jackson Laboratory, 000671), DBA/2NCrl (Charles River Laboratories, 026) and DBA/2NTac (Taconic Biosciences, DBA2-F/DBA2-M).

Gaines C.H., Schoenrock S.A., Farrington J., Lee D.F., Aponte-Collazo L.J., Shaw G.D., Miller D.R., Ferris M.T., Pardo-Manuel de Villena F, & Tarantino L.M. (2022). Cocaine-Induced Locomotor Activation Differs Across Inbred Mouse Substrains. Frontiers in Psychiatry, 13, 800245.

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Murine Neuronal Culture and In Utero Electroporation

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For the neuronal cultures, FvB/NHsD females were crossed with FvB/NHsD males (both ordered at 8–10 weeks old from Envigo). For the in utero electroporation, female FvB/NHsD (Envigo) were crossed with male C57Bl6/J (ordered at 8–10 weeks old from Charles River). All mice were kept group‐housed in IVC cages (Sealsafe 1145T, Tecniplast) with bedding material (Lignocel BK 8/15 from Rettenmayer) on a 12/12 h light/dark cycle in 21°C (±1°C), humidity at 40–70% and with food pellets (801727CRM(P) from Special Dietary Service) and water available ad libitum. All animal experiments were approved by the Local Animal Experimentation Ethical Committee, in accordance with Institutional Animal Care and Use Committee guidelines.

Proietti Onori M., Koopal B., Everman D.B., Worthington J.D., Jones J.R., Ploeg M.A., Mientjes E., van Bon B.W., Kleefstra T., Schulman H., Kushner S.A., Küry S., Elgersma Y, & van Woerden G.M. (2018). The intellectual disability‐associated CAMK2G p.Arg292Pro mutation acts as a pathogenic gain‐of‐function. Human Mutation, 39(12), 2008-2024.

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Murine Neuronal Culture and In Utero Electroporation

Check if the same lab product or an alternative is used in the 5 most similar protocols

For the neuronal cultures, FvB/NHsD females were crossed with FvB/NHsD males (both ordered at 8–10 weeks old from Envigo). For the in utero electroporation female FvB/NHsD (Envigo) were crossed with male C57Bl6/J (ordered at 8–10 weeks old from Charles River). All mice were kept group-housed in IVC cages (Sealsafe 1145T, Tecniplast) with bedding material (Lignocel BK 8/15 from Rettenmayer) on a 12/12 h light/dark cycle in 21 °C (±1 °C), humidity at 40–70% and with food pellets (801727CRM(P) from Special Dietary Service) and water available ad libitum. All animal experiments were approved by the Erasmus MC institutional Animal Care and Ethical Committee, in accordance with European and Institutional Animal Care and Use Committee guidelines.

Reijnders M.R., Kousi M., van Woerden G.M., Klein M., Bralten J., Mancini G.M., van Essen T., Proietti-Onori M., Smeets E.E., van Gastel M., Stegmann A.P., Stevens S.J., Lelieveld S.H., Gilissen C., Pfundt R., Tan P.L., Kleefstra T., Franke B., Elgersma Y., Katsanis N, & Brunner H.G. (2017). Variation in a range of mTOR-related genes associates with intracranial volume and intellectual disability. Nature Communications, 8, 1052.

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