Legend max elisa kit

ELISA analysis was performed for the characterization of the immune response upon LPS stimulation. Supernatant of cells incubated with LPS (time points as indicated above) was used for the quantification of IL-6, TNF-α, and IL-10 using the Legend Max ELISA kits (BioLegend, San Diego, CA, USA). By using a calibration series, a concentration in pg/mL for each cytokine could be derived.

The ELISA kits were purchased from BioLegend (San Diego, CA, USA) and were used for quantification of the proteins in the blood samples. In the present study, the ELISA kits used were as follows: LEGEND MAX™ Human IL-33 ELISA Kit (cat.no. 435907), LEGEND MAX™ Human IL-8 ELISA Kit (cat. no. 431507), and LEGEND MAX™ Human IL-17A ELISA Kit (cat.no. 433917). BioLegend’s LEGEND MAX™ ELISA kits are a sandwich enzyme-linked immunosorbent assay (ELISA) with a 96-well strip plate that is precoated with a capture antibody. The kits are specifically designed for the accurate protein quantitation of human IL-33, human IL-17A, and human IL-8 from serum samples used in our study. Serum levels of IL-33, IL-17A, and IL-8 were determined by ELISA according to the manufacturer’s instructions (BioLegend, San Diego, CA, USA). Concentrations were calculated by comparison with standard curves. For more accurate results, by avoiding interaction between analytes during the testing process, molecules were tested individually and not by multiplexed analysis. A Biotek Synergy H1 hybrid absorbance microplate reader was used to perform ELISA testing on interleukins 8, 17A, and 33.