Ab109251

Manufactured by Abcam

Sourced in United States, United Kingdom

Ab109251 is a primary antibody produced in rabbit that targets the human UGT1A1 protein. It is intended for use in Western blotting applications to detect the expression of UGT1A1.

Automatically generated - may contain errors

Lab products found in correlation

Bm0627, by Boster Bio (1 mentions) Pvdf membrane, by Merck Group (1 mentions) Sc 47778, by Santa Cruz Biotechnology (1 mentions) Ab45422, by Abcam (1 mentions) Ab2433, by Abcam (1 mentions) Fusion fx software, by Vilber (1 mentions) Criterion apparatus, by Bio-Rad (1 mentions) Ab86071, by Abcam (1 mentions) Ab9484, by Abcam (1 mentions) Ponceau s stain, by Merck Group (1 mentions)

3 protocols using ab109251

Western Blot Analysis of Adipose and Energy Metabolism

Cited 1 time

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The standard procedure for Western blotting was performed using the following antibodies: anti-adipose triglyceride lipase antibody (abcam, ab109251, Cambridge, MA, USA), anti-AMPKα antibody (CST, mAb #2603, Danvers, MA, USA), anti-phospho-AMPKα antibody (CST, mAb #2535), anti-SREBP1 antibody (Novus Biologicals, NB-100-2215SS, Littleton, CO, USA), anti-β-actin antibody (Boster, BM0627, Wuhan, China). Western blot analysis was done as described elsewhere [43 (link)]. Briefly, whole-tissue and cell lysates were prepared, and protein concentrations were measured using the bicinchoninic acid (BCA) method. Proteins were separated on the Bio-Rad Mini-PROTEAN Tetra gel system (BioRad, Hercules, CA, USA). The proteins were transferred to PVDF membranes and probed. Equal loading of the protein was confirmed by β-actin.

Tan J., Huang C., Luo Q., Liu W., Cheng D., Li Y., Xia Y., Li C., Tang L., Fang J., Pan K., Ou Y., Cheng A, & Chen Z. (2019). Soy Isoflavones Ameliorate Fatty Acid Metabolism of Visceral Adipose Tissue by Increasing the AMPK Activity in Male Rats with Diet-Induced Obesity (DIO). Molecules, 24(15), 2809.

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Western Blot Analysis of Adipose Tissue Proteins

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Proteins from rat visceral white adipose tissue were prepared using lysis buffer. Equal amounts of protein were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene fluoride (PVDF) membranes (Merck Millipore, Burlington, MA, USA). The membranes were blocked using 5% dried skimmed milk powder in TBS for 2 h at 37°C. The membranes were incubated overnight at 4°C with primary antibodies to: uncoupling protein-1 (UCP-1) (1: 500) (ab2433; Abcam, Cambridge, UK), hormone-sensitive lipase (HSL) (1: 5,000) (ab45422; Abcam, Cambridge, UK), adipose triglyceride lipase (ATGL) (1: 2,000) (ab109251; Abcam, Cambridge, UK), and GAPDH (1: 1,000) (sc-47778; Santa Cruz Biotechnology Inc., Dallas, TX, USA). Membranes were washed and incubated with the secondary antibody for 1 h at 25°C. Protein bands were detected using an electrochemiluminescence (ECL) microplate reader and FUSION Fx software (Vilber Lourmat, Marne-la-Vallée, France). The relative protein expression was calculated with GAPDH used as the control protein.

Gulan T., Yeernuer T., Sui S, & Mayinuer N. (2019). A Rat Model of Maternal Polycystic Ovary Syndrome Shows that Exposure to Androgens In Utero Results in Dysbiosis of the Intestinal Microbiota and Metabolic Disorders of the Newborn Rat. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 25, 9377-9391.

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Western Blot Protein Quantification

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Protein content was measured from total protein extracts by western immunoblotting using the Criterion apparatus and 12.5% SDS-polyacrylamide gels (all purchased from Bio-Rad, Hercules, CA) and adjusted to either GAPDH (AB9484; AbCam, Cambridge, MA) or total protein assessed by Ponceau S stain (Sigma, St. Louis, MO). The antibody for PLIN3 was purchased from Novus Biologicals (Cat no. NB110-40764, Littleton, CO). The antibodies for GBF1 (AB86071), ATGL (AB109251), and ARFRP1 (AB108199) were purchased from AbCam (Cambridge, MA). The antibody for ARF1 was purchased from Epitomics (Cat no. 1635-1; Burlingame, CA).

Covington J.D., Galgani J.E., Moro C., LaGrange J.M., Zhang Z., Rustan A.C., Ravussin E, & Bajpeyi S. (2014). Skeletal Muscle Perilipin 3 and Coatomer Proteins Are Increased following Exercise and Are Associated with Fat Oxidation. PLoS ONE, 9(3), e91675.

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