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Trehalose dihydrate

Manufactured by Biosynth

Trehalose dihydrate is a white crystalline disaccharide compound. It has the chemical formula C₁₂H₂₂O₁₁·2H₂O. Trehalose dihydrate is a naturally occurring sugar that serves as a stabilizer for biological samples and materials.

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2 protocols using trehalose dihydrate

1

Synthesis and Characterization of Polyampholyte Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols
DMSO (analytical reagent grade), poly(methyl vinyl ether-alt-maleic anhydride) (average Mn ≈ 80,000 Da, impurities: <2% benzene), Dulbecco’s phosphate-buffered saline (1.15 g L−1 dibasic sodium phosphate, 0.2 g L−1 potassium chloride, 8 g L−1 sodium chloride, and 0.2 g L−1 monobasic potassium phosphate) (DPBS) were purchased from Sigma-Aldrich. Trehalose dihydrate (purity >98%) was purchased from Carbosynth. Poly-(ethylene glycol) (PEG) (average Mn = 4000 g mol−1) was purchased from Sigma-Aldrich. 2-Dimethylaminoethanol (purity 99%) was purchased from Acros Organics. Tetrahydrofuran (laboratory reagent grade) was purchased from Merck. BD FACSFlow sheath fluid was purchased from BD biosciences. Sheep’s blood in Alsever’s solution (not defibrinated) was from TCS Biosciences. Polyampholyte (poly(vinyl ether-alt-maleic acid mono(dimethylamino ethyl)ester)) was synthesized in house as described in the Supporting Information Milli-Q ultrapure water (>18.2 MΩ cm−1 2 ppb) was used throughout.
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2

Polymer-Based Cryopreservation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
DMSO (analytical reagent grade),
poly(methyl vinyl ether-alt-maleic anhydride) (average
Mn ≈ 80,000 Da, impurities: <2% benzene), Dulbecco’s
phosphate-buffered saline (1.15 g L–1 dibasic sodium
phosphate, 0.2 g L–1 potassium chloride, 8 g L–1 sodium chloride, and 0.2 g L–1 monobasic
potassium phosphate) (DPBS) were purchased from Sigma-Aldrich. Trehalose
dihydrate (purity >98%) was purchased from Carbosynth. Poly(ethylene
glycol) (PEG) (average Mn = 4000 g mol–1) was purchased
from Sigma-Aldrich. 2-Dimethylaminoethanol (purity 99%) was purchased
from Acros Organics. Tetrahydrofuran (laboratory reagent grade) was
purchased from Merck. BD FACSFlow sheath fluid was purchased from
BD biosciences. Sheep’s blood in Alsever’s solution
(not defibrinated) was from TCS Biosciences. Polyampholyte (poly(vinyl
ether-alt-maleic acid mono(dimethylamino ethyl)ester))
was synthesized in house as described in the Supporting Information Milli-Q ultrapure water (>18.2 MΩ cm–1 2 ppb) was used throughout.
+ Open protocol
+ Expand

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