Blood samples were collected in the morning, after an overnight fast. Plasma total cholesterol (TC), HDL-C, TG and glucose concentrations were determined on a Beckman Synchron Cx7 instrument as previously described [43 (link),44 (link)]. Apolipoprotein (apo) A-I and apo B were measured by nephelometry (Array Protein System; Beckman). The Friedewald equation was used to calculate low-density lipoprotein-cholesterol (LDL-C). Plasma insulin concentration was determined with the ultrasensitive Access® immunoassay system (Beckman Coulter, Inc.), which has no cross-reactivity with proinsulin or C-peptide. Plasma free fatty acids (FFA) concentrations were quantified by an enzymatic colorimetric method (Wako Chemicals).
Array protein system
Manufactured by Beckman Coulter
Sourced in United States
The Array Protein System is a multiplex assay platform designed for the simultaneous detection and quantification of multiple proteins in a single sample. It utilizes bead-based technology to enable highly sensitive and specific protein measurements. The system is capable of analyzing a wide range of protein targets, making it a versatile tool for various applications in research and clinical settings.
Automatically generated - may contain errors
3 protocols using array protein system
1
Plasma Lipid and Glucose Biomarkers
2
Measurement of HbA1c, Glucose, Albuminuria
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HbA1c was measured at baseline and each visit using the high-performance liquid chromatography technique (Diamat, Bio-Rad, Munich, Germany). The examiners were not blinded to the HbA1c results at the individual time points. Capillary blood glucose was measured by a hexokinase-based method (ACP 5040 autoanalyzer, Eppendorf, Hamburg, Germany; Cobas C 311 analyzer, Roche Diagnostics, Mannheim, Germany). Albuminuria was measured in 12 h urine samples using the immunonephelometric technique (Array Protein System, Beckman, Fullerton, California, USA) or turbidimetric method (Cobas C 311 analyzer, Roche Diagnostics, Mannheim, Germany) with a normal range: <20 µg/min. Islet cell antibodies were measured by indirect immunofluorescence using snap frozen, unfixed human pancreatic sections of blood 0 donors.17 (link)
3
Lipid and Metabolic Biomarkers in Children
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Blood samples were collected in the morning, after an overnight fast. Plasma total cholesterol (TC), HDL-C, TG, and glucose concentrations were determined on a Beckman Synchron Cx7 instrument as previously described (18) . Apolipoprotein (apo) A-I and apo B were measured by nephelometry (Array Protein System; Beckman). The Friedewald equation was used to calculate LDL-C. Plasma insulin concentrations were determined with the ultrasensitive Access immunoassay system (Beckman Coulter, Brea, CA, USA), which has no cross-reactivity with proinsulin or C-peptide. Plasma FFA concentrations were quantified by an enzymatic colorimetric method (Wako Chemicals, Richmond, VA, USA). Plasma LTF was measured using a commercial ELISA kit (AssayPro, St Charles, MO) in a sample of 176 children aged 9, 13, and 16 years, selected according to their extreme HDL-C values in order to detect the largest effect.
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