Human IL-18 ELISA Kit (ab215539), Mouse IL-18 ELISA Kit (ab216165), Human IL-1β ELISA Kit (ab214025), Mouse IL-1β ELISA Kit (ab197742), Mouse S100A8 ELISA Kit (ab263886), Mouse S100A9 ELISA Kit (ab213887) and HSP70 ELISA Kit (ab133060) were purchased from Abcam, UK. Human S100A8 (E-EL-H1289c), Human S100A9 (E-EL-H1290c), HSP90 ELISA Kit (E-EL-H1864c), Human HMGB-1 ELISA Kit (E-EL-H1554c), Mouse HMGB-1 ELISA Kit (E-EL-M0676c) were obtained from Elabscience Biotechnology Co., Ltd, China. ATP Assay Kit (S0027) was purchased from Beyotime, China.
Briefly, differentiated U937 or RAW264.7 was stimulated with LPS for 6 h. Next, TDCA was added to the medium at the final concentration of 200 μM for 24 h. Subsequently, the supernatant was collected to measure the level of DAMPs including HSP70, HSP90, S100A8, S100A9, and ATP. All assays were performed following the instructions of the producer.
Briefly, differentiated U937 or RAW264.7 was stimulated with LPS for 6 h. Next, TDCA was added to the medium at the final concentration of 200 μM for 24 h. Subsequently, the supernatant was collected to measure the level of DAMPs including HSP70, HSP90, S100A8, S100A9, and ATP. All assays were performed following the instructions of the producer.