The cell surface markers of MSCs were analyzed using a FACS Calibur flow cytometer (BD Biosciences, San Jose, CA, USA). Briefly, cells that reached 90% confluence were harvested using 0.25% EDTA and washed twice in Dulbecco’s phosphate buffered saline supplemented with 10% FBS. The cells for detecting CD11b, CD34, CD45, Sca-1, CD44 and CD73 were labeled directly with BB515 or PE-conjugated CD markers (rat anti-mouse CD11b [1: 100, BD Pharmingen; BD Biosciences, Franklin Lake, NJ, USA], rat anti-mouse CD34 [1: 100, BD Pharmingen], rat anti-mouse CD45 [1: 100, BD Pharmingen], rat anti-mouse Sca-1 [1: 100, BD Pharmingen], rat anti-mouse CD44 [1: 100, BD Pharmingen], rat anti-mouse CD73 [1: 100, BD Pharmingen]).
Rat anti mouse cd34
Manufactured by BD
Sourced in United States
Rat anti-mouse CD34 is a monoclonal antibody that recognizes the CD34 antigen, which is a surface glycoprotein expressed on hematopoietic stem and progenitor cells. This antibody can be used for the identification and isolation of mouse CD34-positive cells.
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Lab products found in correlation
Rat anti mouse cd45, by BD (1 mentions)
Rat anti mouse cd11b, by BD (1 mentions)
Rat anti mouse sca1, by BD (1 mentions)
Rat anti mouse cd44, by BD (1 mentions)
Facscalibur, by BD (1 mentions)
Cd11b pe cy7, by Thermo Fisher Scientific (1 mentions)
Ly6c apc, by Thermo Fisher Scientific (1 mentions)
V450 mouse lineage antibody cocktail, by BD (1 mentions)
Facscanto 2, by BD (1 mentions)
2 protocols using rat anti mouse cd34
1
Characterization of Mouse Mesenchymal Stem Cells
2
Bone Marrow Stem Cell Isolation
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BM was harvested from femurs by flushing with ice cold RPMI. The cell suspension was centrifuged at 800 g for 5 min, and supernatant aspirated. The cells were resuspended in RBC lysis buffer for 3 min and adding PBS with 1% FBS buffer and once again centrifuged. Cells were incubated with V450 mouse lineage antibody cocktail (561301, BD), in incubation with rat anti mouse Ly-6A/E (Sca-1) (558162, BD) and Rat anti mouse CD117 (c-kit) (553356, BD) antibodies, to quantify the percentage of Lin− Sca1+cKit+ (LSK) Cells. Another population of cells from BM was stained with rat anti mouse CD34 (560238, BD) to identify the population of LT-HSCs and short-term (ST)-HSCs. The antibodies used for the MDSCs experiments were CD11b-PEcy7 (25-0112-82, eBioscience), Ly6G-eflur 450 (45-5931-82, eBioscience), and Ly6C-APC (17-5932-82, eBioscience). All antibodies were incubated for 30 min at 4°C and samples were analyzed with FACS Canto II (BD).
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