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Cd11b apc m1 70

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CD11b-APC (M1/70) is a lab equipment product that detects the expression of the CD11b antigen, also known as the integrin alpha M chain or Mac-1 alpha chain. This antibody is conjugated with the fluorescent dye Allophycocyanin (APC) and can be used for flow cytometric analysis.

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Lab products found in correlation

Facscalibur, by BD (3 mentions) F4 80 pe bm8, by Thermo Fisher Scientific (3 mentions) C kit apc 2b8, by BD (2 mentions) Diva version 6, by BD (1 mentions) Lsr 2 cytometer, by BD (1 mentions) Cd34 pe cy7, by BioLegend (1 mentions) Celesta, by BD (1 mentions) Ly6c fitc al 21, by BD (1 mentions) F4 80 pe bm8, by BioLegend (1 mentions) Mhcii bv421 m5 114, by BioLegend (1 mentions) Cd45 bv510 30 f11, by BioLegend (1 mentions) Ly6g pe cy7, by BioLegend (1 mentions) Siglecf pe cf594, by BD (1 mentions) Fixable viability dye efluor 780, by Thermo Fisher Scientific (1 mentions) Ly6g pe 1a8, by BD (1 mentions) Msu crystals, by Enzo Life Sciences (1 mentions) Kineret, by Amgen (1 mentions) Apoferritin, by Merck Group (1 mentions) 7 aad, by Thermo Fisher Scientific (1 mentions) Immersol medium, by Zeiss (1 mentions)

Spelling variants of this product

CD11b (360 citations) CD11b (M1/70, (82 citations) CD11b-APC (77 citations) M1/70 (35 citations) CD11b APC (clone M1/70), (8 citations) Anti-CD11b-APC (clone M1/70, (5 citations) Anti-CD11b-APC (M1/70, (2 citations) APC-eFluor780-conjugated anti-CD11b (M1/70, (2 citations)

9 protocols using cd11b apc m1 70

1

Flow Cytometry Analysis of Immune Cells

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Flow cytometry analyses were done using a BD-LSRII cytometer and analyzed using BD-DIVA Version 6.1.2 software (BD Biosciences). Antibodies used were CD11B-PE (Mac-1), 3:100, Beckman Coulter; CD11B-APC (M1/70), 1:500, eBioscience; CD11C-PeCy7 (BU-15), 3:100, Beckman Coulter; DRAQ7™, 1:400; Biostatus, CD34-PeCy7 (#343516) 1:33, Biolegend.
Garciaz S., N’guyen Dasi L., Finetti P., Chevalier C., Vernerey J., Poplineau M., Platet N., Audebert S., Pophillat M., Camoin L., Bertucci F., Calmels B., Récher C., Birnbaum D., Chabannon C., Vey N, & Duprez E. (2019). Epigenetic down-regulation of the HIST1 locus predicts better prognosis in acute myeloid leukemia with NPM1 mutation. Clinical Epigenetics, 11, 141.
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2

Comprehensive Flow Cytometry Analysis of Immune Cells

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Colonic LP samples (2 million cells/staining) were stained at 4°C for 20 min with the following antibodies: Ly6C-FITC (AL-21, BD Pharmingen), F4/80-PE (BM8, BioLegend), Siglec F-PE-CF594 (E50-2440, BD Bioscience), Ly6G PE-Cy7 (1A8, BioLegend), CD11b-APC (M1/70, eBioscience), CD11c-AF700 or -APC-R700 (N418, BioLegend), MHCII-BV421 (M5/114.15.2, BioLegend), and CD45-BV510 (30-F11, BioLegend) at 4°C for 20 min. Dead cells were identified by staining with eFluor 780 Fixable Viability dye (eBioscience) and gatings were performed as described previously (22 (link)).
For analyses of integrin receptors on human monocytes, mouse Hoxb8 monocytes and mouse BMDMs, antibodies to integrins β1 (mouse 9EG7 (23 (link))/human P5D5) (24 (link)), α6 (GoH3, BD Pharmingen), α5 (mouse 5H10-27, BD Pharmingen/human P1D6) (24 (link)), α4 (PS/2, Abcam), α3 (mouse polyclonal, R&D/human P1B5) (24 (link)), β3 (mouse 2C9.G2, Biolegend/human B3A, Chemicon), and β2 (mouse C71/16, BD Pharmingen/human TS1/18, Thermo Scientific) were employed. Cells were analyzed with a Gallios (Beckman Coulter) or Celesta (BD) flow cytometer and FlowJo software.
Li L., Song J., Chuquisana O., Hannocks M.J., Loismann S., Vogl T., Roth J., Hallmann R, & Sorokin L. (2020). Endothelial Basement Membrane Laminins as an Environmental Cue in Monocyte Differentiation to Macrophages. Frontiers in Immunology, 11, 584229.
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3

Neutrophil Recruitment in Murine Gout

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8–10-wk old C57BL/6 mice were injected i.p. with anakinra (30 mg/kg, Kineret®, Amgen), P2D7KK (5 mg/kg and 15 mg/kg), isotype control human IgG1 antibody (15 mg/kg), or saline. After 10 min, mice received another i.p. injection of 3 mg MSU crystals (Enzo Life Sciences) in 0.5 mL of saline. Control mice were injected with saline alone. After 6 h, peritoneal exudate cells were collected by lavage with cold medium, centrifuged, and red blood cell lysis was performed using hypotonic ammonium chloride solution for 1 min. Total peritoneal cells were counted, stained with Ly6G PE (1A8, BD PharMingen) and CD11b APC (M1/70, eBioscience), and analyzed by flow cytometry. Neutrophils were identified as Ly6G+CD11b+ cells.
Goh A.X., Bertin-Maghit S., Ping Yeo S., Ho A., Derks H., Mortellaro A, & Wang C.I. (2014). A novel human anti-interleukin-1β neutralizing monoclonal antibody showing in vivo efficacy. mAbs, 6(3), 764-772.
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4

Quantifying Macrophage Phagocytosis via Flow Cytometry

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Bone marrow-derived macrophages (BMDMs) were changed to DMEM + 1% FBS media 24 h before being treated with LPS (100 ng/mL) or Apoferritin (0.1 mg/mL; Sigma). BMDMs were harvested and stained with CD11b-APC (M1/70; eBioscience), 7AAD (eBioscience). Cells were simultaneously incubated with MOI 10 pHrodo Red Escherichia coli BioParticles (ThermoFisher), as previously described with minor modifications (26 (link)). Cells were analyzed on a BD FACSCalibur and data was analyzed using FlowJo software.
Zarjou A., Black L.M., McCullough K.R., Hull T.D., Esman S.K., Boddu R., Varambally S., Chandrashekar D.S., Feng W., Arosio P., Poli M., Balla J, & Bolisetty S. (2019). Ferritin Light Chain Confers Protection Against Sepsis-Induced Inflammation and Organ Injury. Frontiers in Immunology, 10, 131.
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5

Comprehensive Hematopoietic Lineage Staining

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Monoclonal antibodies for lineage staining used were Gr1-PE (clone-RB6-8CS), B220-PE (RA3-6B2), Sca-1-PE (D7), ckit-APC (2B8) and CD45-FITC (HI30) from BD Biosciences, CD11b-APC (M 1/70) and F4/80-PE (BM8) from eBioscience. Antibodies used for the lineage cocktail were PerCP Cy5.5 labelled CD4 (GK1.5) and Ter119 (Ter-119) from Biolegend, B220 (RA3-6B2) and CD3 (145-2C11) from BD Biosciences and Gr-1 (RB6-8C5C), CD8 (53-6.7), IL7R (A7R34), CD11b (M1/70) from eBioscience. Antibodies used for SLAM HSC staining were CD48-APC (HM48-1), CD150-PECy7 (TC-15-12F12.2) from Biolegend and CD244-PE (2B4) from eBioscience. Antibodies used for progenitor staining were CD16/32-PE (93), CD34-biotin (RAM34), Sca1-PECy7 (D7) from eBioscience, ckit-APC (2B8) and Streptavidin-APC-Cy7 from BD Biosciences. Cytospin preparations were stained with Wright-Giemsa stain. The morphology of bone marrow was assessed with an Olympus BX51 (Olympus, Tokyo, Japan) microscope and a 40x/0.75 numerical aperture objective, or a 100x/1.3 numerical aperture objective with Zeiss immersol medium (Zeiss, Jena, Germany). OlympusXC50 (Olympus) and analySIS software (Soft Imaging System, Stuttgart, Germany) were used to capture images.
Chaturvedi A., Araujo Cruz M.M., Jyotsana N., Sharma A., Goparaju R., Schwarzer A., Görlich K., Schottmann R., Struys E.A., Jansen E.E., Rohde C., Müller-Tidow C., Geffers R., Göhring G., Ganser A., Thol F, & Heuser M. (2016). Enantiomer-specific and paracrine leukemogenicity of mutant IDH metabolite 2-hydroxyglutarate. Leukemia, 30(8), 1708-1715.
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6

Isolation and Analysis of Tumor-Derived Myeloid Cells

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Primary tumors were isolated from mice, cut into pieces, and digested in RPMI1640 containing 200 U·mL−1 of collagenase, type I (Worthington, Lakewood, NJ, USA) and 10 μg·mL−1 of DNaseI (Roche Diagnostics) for 60 min at 37 °C on a shaking platform. The samples were then washed and filtered through a cell strainer (100‐μm nylon; Corning, Corning, NY, USA). Red blood cells were lysed in Red Blood Cell Lysis Buffer (Roche Diagnostics). The collected cells were incubated with FcR blocking reagent, mouse (Miltenyi Biotec, Bergisch Gladbach, Germany), for 15 min on ice. For flow cytometry, the cells were stained with the following antibodies for 30 min on ice in the dark: CD11b‐APC (M1/70) and Ly6C‐PE (HK1.4) from eBioscience (San Diego, CA, USA), Ly6G‐PE/Cy7 (1A8) and CD45‐Alexa Fluor 700 (30‐F11) from BioLegend (San Diego, CA, USA), and Gr‐1‐FITC (RB6‐8C5) from BD Biosciences (San Jose, CA, USA). The cells were analyzed by Gallios Flow Cytometer (Beckman Coulter, Fullerton, CA, USA) and were further analyzed using flowjo software (TreeStar software, Ashland, OR, USA).
Katsura A., Tamura Y., Hokari S., Harada M., Morikawa M., Sakurai T., Takahashi K., Mizutani A., Nishida J., Yokoyama Y., Morishita Y., Murakami T., Ehata S., Miyazono K, & Koinuma D. (2017). ZEB1‐regulated inflammatory phenotype in breast cancer cells. Molecular Oncology, 11(9), 1241-1262.
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7

Murine Myeloid Cell Phenotyping

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Total peritoneal cells were labeled with CD11b-APC (M1/70, eBioscience), Ly6C-PE-Cy7 (HK1.4, Biolegend), and Ly6G-PE (1A8, BD PharMingen) for 15 min at room temperature. Cells were then washed with PBS twice before analysis using a Fortessa flow cytometer (BD Biosciences). Data were analyzed using FlowJo (Treestar). Neutrophils were identified as CD11b+ Ly6G+ cells and monocytes as CD11b+ Ly6C+ Ly6G- cells.
Khameneh H.J., Ho A.W., Laudisi F., Derks H., Kandasamy M., Sivasankar B., Teng G.G, & Mortellaro A. (2017). C5a Regulates IL-1β Production and Leukocyte Recruitment in a Murine Model of Monosodium Urate Crystal-Induced Peritonitis. Frontiers in Pharmacology, 8, 10.
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8

Murine Immune Cell Immunophenotyping

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Cells were incubated for 10 min at 4°C with rat anti-mouse CD16/CD32 mAb (1:200, Quantobio, China) at a 1:100 dilution in PBS containing 2% of FBS (Sigma, USA) to prevent nonspecific antibody binding. Subsequently, cells were washed twice in PBS/FBS and incubated for 30 min with 100 μL of each of the following fluorophore-conjugated anti-mouse antibodies: CD11b-APC (M1/70), CD19-APC (MB19-1), CD45-PE-cy7 (30-F11), F4/80-PE (BM8) and Gr-1-FITC (RB6-8C5) (all from eBioscience, USA). Antibodies were used at 1:100 dilution in PBS containing 2% FBS. Data acquisition was performed on a FACS Calibur using CellQuestPro software (BD Biosciences, USA) and analysis carried out using FlowJo software program (Tree Star Inc, USA).
Li W., Zhang X., Chen Y., Xie Y., Liu J., Feng Q., Wang Y., Yuan W, & Ma J. (2016). G-CSF is a key modulator of MDSC and could be a potential therapeutic target in colitis-associated colorectal cancers. Protein & Cell, 7(2), 130-140.
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9

Lineage Determination of Murine Hematopoietic Cells

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Cells were plated at a density of 5 × 105 cells/ml in flat-bottom 6-well plates (5 ml/well) under light-protective conditions and vitC was added at the specified concentrations. Monoclonal antibodies for lineage staining used were Gr1-PE (clone-RB6-8CS), Sca1-PE (D7), ckit-APC (2B8; BD Biosciences, Heidelberg, Germany), CD11b-APC (M1/70) and F4/80-PE (BM8; eBioscience, Frankfurt, Germany). Lineage distribution was determined by fluorescence-activated cell sorting analysis (FACSCalibur, Becton Dickinson, Heidelberg, Germany) as previously described.9 (link)
Mingay M., Chaturvedi A., Bilenky M., Cao Q., Jackson L., Hui T., Moksa M., Heravi-Moussavi A., Humphries R.K., Heuser M, & Hirst M. (2017). Vitamin C-induced epigenomic remodelling in IDH1 mutant acute myeloid leukaemia. Leukemia, 32(1), 11-20.
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