Mouse albumin elisa kit

Manufactured by Aviva Systems Biology

Sourced in United States

The Mouse Albumin ELISA Kit is a quantitative assay designed to measure the concentration of albumin in mouse serum or plasma samples. The kit utilizes the enzyme-linked immunosorbent assay (ELISA) technique to detect and quantify the target analyte.

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Lab products found in correlation

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3 protocols using mouse albumin elisa kit

Bronchoalveolar Lavage Fluid Analysis

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BALF was obtained as previously described (14 (link)). ELISA kits were used to measure serum leptin (Invitrogen, Carlsbad, CA), triacylglycerol (TAG, Pointe Scientific, Canton, MI), insulin (Uppsala, Sweden) and adiponectin (Abcam, Cambridge, UK). Blood glucose was measured via a 6h or 14h fast as described in figure legends. Further, BALF albumin was measured with the Mouse Albumin ELISA Kit (Genway Biotech, Inc., San Diego, CA). Total protein in BALF was measured via standard BCA assay (BCA kit, Sigma-Aldrich, St Louis, MO). BALF cytokines (IL-4, IFN-γ, MCP-1, RANTES, KC, IL-17A, IL-10 and TNF-α) were measured using a BioRad Bio-Plex assay (Hercules, CA) per manufacturer’s instructions. IL-4 was not measureable above the lower limit of the assay.

Milner J.J., Rebeles J., Dhungana S., Stewart D.A., Sumner S.C., Meyers M.H., Mancuso P, & Beck M.A. (2015). Obesity increases mortality and modulates the lung metabolome during pandemic H1N1 influenza virus infection in mice1. Journal of immunology (Baltimore, Md. : 1950), 194(10), 4846-4859.

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Quantifying Albumin in BALF

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The amount of albumin present in the supernatant of our BALF was measured using a Mouse Albumin ELISA kit (GenWay Biotech, Inc.). The BALF supernatant was diluted to a concentration of 1:80,000 using the diluent provided in the kit. The protocol provided in the kit was followed as per the manufacturer’s instructions. The assay was read using the Emax® Endpoint ELISA Microplate Reader from Molecular Devices, set at a wavelength of 450 nm. Data are represented as optical density (O.D.).

Bratt J.M., Chang K.Y., Rabowsky M., Franzi L.M., Ott S.P., Filosto S., Goldkorn T., Arif M., Last J.A., Kenyon N.J, & Zeki A.A. (2018). Farnesyltransferase Inhibition Exacerbates Eosinophilic Inflammation and Airway Hyperreactivity in Mice with Experimental Asthma: The Complex Roles of Ras GTPase and Farnesylpyrophosphate in Type-2 Allergic Inflammation. Journal of immunology (Baltimore, Md. : 1950), 200(11), 3840-3856.

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Quantification of Cytokine and Chemokine Profiles

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Data analysis was performed using the FlowJo TM analysis software (Treestar, Costa Mesa, USA). The BAL supernatant was quantified for murine interleukin 1α (IL-1α), interleukin 1β (IL-1β), granulocytemacrophage colony-stimulating factor (GM-CSF), interleukin 6 (IL-6), tumor necrosis factor α (TNFα), macrophage inflammatory protein 1α (MIP-1α), KC and monocyte chemoattractant protein 1 (MCP-1) with a Bio-Plex ® Suspension array system (Biorad, Hercules, USA) according to manufacturer's specifications. The analytes were measured with a Luminex protein array reader and Bio-Plex ® manager software version 5.0 (Bio-Rad Laboratories), using recombinant standards (Bio-Rad Laboratories). For data analysis, a 5-parameter logistic curve fit was applied to each standard curve and sample. In addition, the supernatant was quantified for mouse albumin using a Mouse Albumin ELISA Kit (Genway Biotech, San Diego, CA, USA).

Merckx P., De Backer L., Van Hoecke L., Guagliardo R., Echaide M., Baatsen P., Olmeda B., Saelens X., Pérez-Gil J., De Smedt S.C, & Raemdonck K. (2018). Surfactant protein B (SP-B) enhances the cellular siRNA delivery of proteolipid coated nanogels for inhalation therapy. Acta biomaterialia, 78.

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