The rabbit polyclonal antibody against RTTN was raised using recombinant RTTN-His (residues 1347–1591) and subjected to affinity purification. The following antibodies were used in this studies: antibodies against SAS-6 (residues 1–489, 1:500 dilution)13 (link), CPAP (residues 1070–1338, 1:1000 dilution)50 (link), CEP120 (residues 639–986, 1:1000 dilution)15 (link), CEP135 (residues 650–1140, 1:1000 dilution)9 (link), CEP295 (residues 2092–2430, 1:500 dilution)19 (link), centrobin (residues 443–626, 1:1000 dilution)13 (link), and centrin 2 (residues 1–173, 1:1000 dilution)13 (link). The other commercially available antibodies used in this study included anti-STIL (A302–442; 1:200 dilution), anti-hPOC5 (A303–341; 1:500 dilution) and anti-SPICE (A303–272; 1:500 dilution) (all from Bethyl); anti-hSAS-6 (H00163786; 1:100 dilution, monoclonal Ab), anti-centrin3 (H00001070-M01; 1:1000 dilution) and anti-CEP162 (PAB22408; 1:1000 dilution) (all from Abnova); anti-CP110 (12780-1-1p; 1:500 dilution; Proteintech); anti-CEP164 (NBP1-81445; 1:500 dilution; Novus Biologicals); anti-POC1B (PA5-24495; 1:500 dilution; Thermo Fisher); anti-acetylated tubulin (T6793; 1:1000 dilution) and anti-Flag (M2; F3165; 1:3000 dilution) (both from Sigma-Aldrich); and anti-GFP (632381; 1:3000 dilution; BD Bioscience).
Anti acetylated tubulin t6793
Manufactured by Merck Group
Sourced in United States
Anti-acetylated tubulin (T6793) is a primary antibody used in research applications. It specifically binds to acetylated forms of the tubulin protein, which is a key component of the cytoskeleton in eukaryotic cells. This antibody can be used to detect and quantify acetylated tubulin levels in various cell and tissue samples.
Automatically generated - may contain errors
Lab products found in correlation
Lsm 710 confocal microscope, by Zeiss (3 mentions)
Anti hpoc5, by Fortis Life Sciences (1 mentions)
Anti flag m2 f3165, by Merck Group (1 mentions)
Anti cep164 nbp1 81445, by Novus Biologicals (1 mentions)
Anti gfp, by BD (1 mentions)
Alexa 488, by Thermo Fisher Scientific (1 mentions)
A11029, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 568 goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 568 donkey anti mouse igg, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 647 goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Anti ift81 11744 1 ap, by Proteintech (1 mentions)
Anti bmpr 2 612292, by BD (1 mentions)
Anti arl13b 17711 1 ap, by Proteintech (1 mentions)
Alexa fluor 488 goat anti mouse igg, by Thermo Fisher Scientific (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Vectashield, by Vector Laboratories (1 mentions)
Fibronectin, by Corning (1 mentions)
3 protocols using anti acetylated tubulin t6793
1
Centrosome Protein Antibody Protocol
2
Whole-Mount Immunofluorescence of Embryos
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The embryos at the desired developmental stage were fixed with 4% PFA overnight at 4 °C. The embryos were subsequently dehydrated in an increasing series of methanol until transferred to 100% methanol and stored at −20 °C. Following rehydration, IHC protocol was followed as previously described [45 (link)]. The primary antibodies used were anti-acetylated tubulin (T6793, Sigma) at 1:400 and anti-myosin (A4.1025, deposited to the DSHB by Blau, H.M., University of Yowa, Iowa City, IA, USA) at 1:50, and the secondary antibodies used were a goat anti-mouse Alexa488 (A11029, Invitrogen, Waltham, MA, USA) at 1:200 in blocking solution. For nuclear staining, DAPI (4′,6-diamidino-2-phenylindole; D1306, Invitrogen) at 30 mg/mL was added to the secondary antibody’s solution.
The embryos were mounted in a drop of 1% low melting agarose (16520-050, Invitrogen) in 1 × PBS for correct embryo orientation and were imaged using a water dipping lens in a Zeiss LSM710 confocal microscope.
The embryos were mounted in a drop of 1% low melting agarose (16520-050, Invitrogen) in 1 × PBS for correct embryo orientation and were imaged using a water dipping lens in a Zeiss LSM710 confocal microscope.
3
Immunofluorescence Staining of HUVECs
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HUVECs grown on 8-well cell culture slides (Mat-Tek) coated with Fibronectin (Corning, Sigma-Aldrich)
were washed with warmed PBS (37 ℃) twice, fixed in warm 4% paraformaldehyde (Thomas Scientific, 37 ℃) for 5 min and washed with PBS 3 times. The cells were permeabilized with 1% Triton X-100 in PBS for 5 min and washed again with PBS 3 times. The cells were blocked with 1% bovine serum albumin (Sigma-Aldrich) in PBS for 30 min. Anti-acetylated-tubulin (T6793, Sigma-Aldrich, 1:1000), anti-Arl13B
(17711-1-AP, Proteintech, 1:500), anti-IFT81 (11744-1-AP, Proteintech, 1:500) and anti-BMPR-II (612292, BD Biosciences, 1:500) antibodies were incubated overnight at 4℃, and then detected with Alexa Fluor 568 donkey anti-mouse IgG (A10037, Invitrogen, 1:500), Alexa Fluor 568 goat anti-rabbit IgG (A11011, Invitrogen, 1:200), Alexa Fluor 488 goat anti-mouse IgG (A11001, Invitrogen, 1:200) and/or Alexa Fluor 647 goat anti-rabbit IgG (A21244, Invitrogen, 1:200). 0.5 µg/ml DAPI (Sigma-Aldrich) was used to stain the nuclei. The cells were mounted using Vectashield (Vector Labs) and imaged using fluorescence microscopy.
were washed with warmed PBS (37 ℃) twice, fixed in warm 4% paraformaldehyde (Thomas Scientific, 37 ℃) for 5 min and washed with PBS 3 times. The cells were permeabilized with 1% Triton X-100 in PBS for 5 min and washed again with PBS 3 times. The cells were blocked with 1% bovine serum albumin (Sigma-Aldrich) in PBS for 30 min. Anti-acetylated-tubulin (T6793, Sigma-Aldrich, 1:1000), anti-Arl13B
(17711-1-AP, Proteintech, 1:500), anti-IFT81 (11744-1-AP, Proteintech, 1:500) and anti-BMPR-II (612292, BD Biosciences, 1:500) antibodies were incubated overnight at 4℃, and then detected with Alexa Fluor 568 donkey anti-mouse IgG (A10037, Invitrogen, 1:500), Alexa Fluor 568 goat anti-rabbit IgG (A11011, Invitrogen, 1:200), Alexa Fluor 488 goat anti-mouse IgG (A11001, Invitrogen, 1:200) and/or Alexa Fluor 647 goat anti-rabbit IgG (A21244, Invitrogen, 1:200). 0.5 µg/ml DAPI (Sigma-Aldrich) was used to stain the nuclei. The cells were mounted using Vectashield (Vector Labs) and imaged using fluorescence microscopy.
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