Cells were dispensed into 96-well microplates at 5 × 104 cells in 200 µL per well. After overnight culture, cells were treated with the indicated concentrations of TBE for an additional 48 h. Cells were washed in DMEM with 10% FBS and then incubated with 5 µg/mL MTT (345-01821, Dojindo, Tokyo, Japan) in 100 µL medium for 2 h. To lyse MTT-generated crystals, 100 µL of 10% sodium dodecyl sulfate was added after the removal of the MTT medium, and the solution was incubated at 37 °C. Twenty hours later, plates were assessed by spectrophotometry at 570 nm.
Methyl thiazolyl tetrazolium (mtt)
Manufactured by Dojindo Laboratories
Sourced in Japan, United States
MTT is a colorimetric assay reagent used to measure cell proliferation, viability, and cytotoxicity. It is a soluble tetrazolium salt that is reduced by metabolically active cells to an insoluble purple formazan product, which can be quantified spectrophotometrically.
Automatically generated - may contain errors
Lab products found in correlation
Multiskan fc, by Thermo Fisher Scientific (6 mentions)
Infinite m1000, by Tecan (4 mentions)
Dimethyl sulfoxide (dmso), by Fujifilm (3 mentions)
Varioskan lux, by Thermo Fisher Scientific (2 mentions)
Microplate reader, by Tecan (2 mentions)
Methyl thiazolyl tetrazolium (mtt), by Tecan (2 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (2 mentions)
Infinite 200 pro microplate reader, by Tecan (2 mentions)
Varioskan flash, by Thermo Fisher Scientific (1 mentions)
Dulbecco s phosphate buffered saline d pbs, by Fujifilm (1 mentions)
Naaso2, by Fujifilm (1 mentions)
Dmi1 inverted microscope, by Leica Microsystems (1 mentions)
Multiskan fc microplate reader, by Thermo Fisher Scientific (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Fujifilm (1 mentions)
Giemsa solution, by Merck Group (1 mentions)
Mitochondrial ros detection kit, by Cayman Chemical (1 mentions)
Ldh cytotoxicity detection kit, by Takara Bio (1 mentions)
Celltiter glo luminescent cell viability assay, by Promega (1 mentions)
Trypan blue dye, by Fujifilm (1 mentions)
Ldh assay kit, by Dojindo Laboratories (1 mentions)
45 protocols using methyl thiazolyl tetrazolium (mtt)
1
Cell Viability Assay with TBE Treatment
2
Peptide Cytotoxicity Evaluation in Cell Lines
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HeLa cells were seeded at 104 cells/well in a 96-well plate. PC12 and HEK293T cells were seeded at 4 × 104 or 104 cells/well, respectively, in PEI-coated 96-well plates. After overnight incubation, cells were treated with 2–30 μM peptides diluted in Opti-MEM for 2 h. Media were replaced with serum-containing RPMI 1640, and cells were treated with 100 or 150 μM CoCl2 for 16 h. After washing with PBS, cells were incubated in 0.5 mg/ml MTT (Dojindo, Kumamoto, Japan) in culture media for 3 h. MTT formazan was dissolved in dimethyl sulfoxide and photometrically quantified at 535 nm. The toxicity of peptides was calculated as described18 (link).
3
Proliferation Assay of Huh7 and 7404 Cells
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Huh7 and 7404 cells were counted with a hemocytometerand placed in 96-well plates (1,000 cells/well) in triplicate and incubated with fresh DMEM medium (containing 10% FBS) at 37°C for 0, 1, 3, 5 or 7 days. MTT (Dojindo Molecular Technologies Inc. Rockville, MD, USA) Cells were incubated with MTT (200 µl/ml medium) for 4 h and subsequently cultured for 0, 1, 3, 5 and 7 days to assess cell proliferation. Cell proliferation curves were determined once absorbance at 540 nm was measured using a microplate reader.
4
MTT Assay for Evaluating PC12 Cell Viability
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Cell viability was assessed using 3-(4, 5-dimethylthiazol-2-yl)-2, 5- diphenyltetrazolium bromide (MTT) assay. PC12 cells were seeded at 1 × 105 cells/ml into poly L-lysine-coated 96-well plates (Wako). After overnight incubation, cells were treated with or without ROEO at 5, 10, 50 and 100 μg/ml for 48 h. MTT assay was then performed by adding 10 μl of MTT (Dojindo, Japan) solution and incubating the cells further for 24 h at 37 °C in the dark., followed by addition of 100 μl of 10% SDS (Wako) and incubation for 24 h. The absorbance at 570 nm was determined using a multidetection microplate reader (Powerscan HT, Dainippon Pharmaceutical, Osaka, Japan). The viability of PC12 cells was calculated as percentage of control.
5
MTT Assay for Cell Viability
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Cells were seeded in a 24-well plate, and the compounds were added. After incubation for 3 days, a solution (1.1 mg/mL) of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT, from DOJINDO Laboratories, Kumamoto, Japan) was added, and MTT assay was performed as previously described [44 (link)].
6
Colorimetric Viability Assay for Cultured Cells
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The MTT method, which measures the viability of cultured cells by colorimetry, was used to determine enzyme activity by reducing MTT (3-(4,5-di-methylthiazol-2yl)-2,5-diphenyltetrazolium bromide) to formazan dye. In both cell types, the number of cells was adjusted to 1.0×10 5 cells/mL, and the cells were seeded in a 96-wellmultiplate and subjected to 24 h static culture in a CO2 incubator. MTT (Dojindo Laboratories) was dissolved in D-PBS to reach a concentration of 0.5 mg/mL; this MTT solution was aliquoted at 100 μL/well in the 96-wellmultiplate and cultured in a CO 2 incubator. The test solution was discarded, the formazan dye was eluted with an acidic isopropanol solution, and the absorbance at 570 nm was measured using an absorptiometer. The calculated value of each sample was divided by the value of the control group and presented as a percentage. This experiment was performed four times.
7
Butryroside D Inhibits Cell Proliferation
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The effect of Butryroside D on B16F10 or HEM cells proliferation was assessed with an MTT assay. Briefly, the cells were seeded into 96-well plates (5 × 104 cells/well) and incubated for 24 h or 48 h, respectively, for B16F10 or HEM at 37 °C in a humidified atmosphere of 5% CO2. Growth mediums were then replaced with 90 μL/well of Butyroside D treatment diluted with RPMI 1640 medium or melanocyte growth medium with a concentration range of 0.2–10 μM kept for 48 h. Then, 10 μL of 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) (Dojindo, Japan) (5 mg/mL) was added to each well and further incubated for 8 h. Lastly, 10% Sodium dodecyl sulfate (SDS) (Fujifilm Wako, Osaka, Japan), 100 μL/well, was added, and the plates were incubated overnight. The absorbance was measured at 570 nm using a microplate reader (Varioskan™ LUX, Thermo Fisher Scientific, Waltham, MA, USA).
8
Assessing NK-exos Viability Effects on HCC
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To assess the viability effects of NK-exosIL−15/21 in HCC cells, a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Dojindo Molecular Technologies, #298-93-1) assay was conducted following the manufacturer's instructions. Hep3B cells (1 × 104 cells/well) were co-treated with exosomes (50 μg) for 24 h. MTT solution (10 μL) was added to each well and re-incubated for 3 h. Thereafter, the medium was removed, and dimethyl sulfoxide (DMSO, 100 μL) was added. To determine cell viability, the absorbance was then read at 570 nm using a microplate reader (Varioskan Flash, Thermo Fisher Scientific, #0250030).
9
MTT Assay for Cell Viability
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THP-1 cells were treated with TM and TQ as described above. Cell viability was assessed by adding 8 µg/ml 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Dojindo Laboratories) to the media. Zinc dibutyldithiocarbamate (ZDBC, 1 µg/ml, Wako Pure Chemical Corporation) was used as a positive control.
10
MTT Cell Viability Assay on Transfected Liver Cells
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MTT experiments were conducted to determine the cell viability of transfected Huh7 and SNU387 cells. Briefly, Huh7 and SNU387 cells were plated in 96-well plates (3 × 103 cells/well). After 48 h, 20 μL of MTT (Dojindo Laboratories, Kumamoto, Japan) was added into 96-well plates. Then, 150 μL of dimethyl sulfoxide (DMSO) was used to dissolve the formazan. The absorbance at 490 nm was recorded under an automatic microplate reader (Applied Biosystems).
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