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Microtof

Manufactured by Bruker
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Sourced in Germany, United States, United Kingdom, Japan

The MicroTOF is a high-performance Time-of-Flight (TOF) mass spectrometer designed for accurate mass determination. It features a compact design and delivers high sensitivity and resolution for a wide range of analytical applications.

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Lab products found in correlation

Uv 2550, by Jasco (2 mentions) V 670 spectrometer, by Jasco (2 mentions) Avance 3 hd spectrometer, by Bruker (2 mentions) Multiskan fc spectrophotometer, by Thermo Fisher Scientific (2 mentions) Ir affinity 1 spectrometer, by Shimadzu (2 mentions) Avance 3 400 mhz nmr spectrometer, by Bruker (2 mentions) Tsq quantum access max mass spectrometer, by Thermo Fisher Scientific (2 mentions) 6130 quadrupole lc ms, by Agilent Technologies (2 mentions) Xbridge c18 column, by Waters Corporation (2 mentions) Preparative hplc system, by Waters Corporation (2 mentions) 1200 series hplc, by Agilent Technologies (2 mentions) Silica gel 60, by Merck Group (2 mentions) Av 400, by Bruker (2 mentions) Cary 300, by PerkinElmer (2 mentions) Tlc silica gel 60 f254, by Merck Group (2 mentions) Avance 400, by Bruker (2 mentions) Spectrum one spectrometer, by PerkinElmer (2 mentions) Ultimate 3000, by Thermo Fisher Scientific (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Nanodrop 2000 spectrophotometer, by Thermo Fisher Scientific (2 mentions)

131 protocols using microtof

1

Carotenoid Profiling by HPLC-MS/MS

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The analysis of the crude carotenoid fraction with high performance liquid chromatography coupled to mass spectrometry (HPLC-MS/MS) was performed in a MicrOTOF Bruker® Daltonics GmbH mass spectrometer using APCI ionization coupled to an Agilent® Infinity 1260 chromatograph.
Chromatography: ACE-3 C18 column (pre-column), dimensions 100 × 2.1 mm, 2.7 μm, at 25 °C, flow rate of 0.4 ml/min at wavelengths 408, 440 and 450 nm, using a linear gradient of methanol in MTBE as the mobile phase (step A: methanol, step B: MTBE, gradient: 0 min = 0% B, 20 min = 20% B, 24 min = 50% B, 30 min. = 100% B). Mass spectrometry conditions: APCI ionization, positive mode, flow 0.240 ml/h, scanning 120–1200 m/z, capillary 3500 V, nebulizer 0.6 Bar, N2 temperature: 180 °C and flow rate of N2: 5 l/min. The identification of the substances was carried out based on the fragmentation profiles in comparison with literature data. The mass measurement was made at a resolution of 20.000.
Reis-Mansur M.C., Cardoso-Rurr J.S., Silva J.V., de Souza G.R., Cardoso V.D., Mansoldo F.R., Pinheiro Y., Schultz J., Lopez Balottin L.B., da Silva A.J., Lage C., dos Santos E.P., Rosado A.S, & Vermelho A.B. (2019). Carotenoids from UV-resistant Antarctic Microbacterium sp. LEMMJ01. Scientific Reports, 9, 9554.
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2

Peptide Synthesis and Characterization

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The peptides were synthesized using an automated ResPepXL peptide synthesizer (Intavis AG, Bioanalytical Instruments) and operated with a Multiple-Parallel Peptide Synthesis Program and were purified by HPLC using Shimadzu LC-20AT, column TSK gel amide-80 (3 µm, 300 x 7.8 mm) equipped by an UV photodiode array detector (SPD-M20A). The UV detection was performed at 214 nm. The analysis by HPLC were performed using the same equipment with a column TSK gel amide-80 (3 µm, 150 × 4.6 mm). 1 (link) H NMR spectra were recorded on a Brucker Advance III 400 MHz spectrophotometer. The spectra were recorded in DMSO-d6 at room temperature (T = 298 K) and residual peak of DMSO (δ = 2.5 ppm) was used as internal reference. The chemical shifts (δ) are given in parts per million (ppm). High resolution mass spectrometry (HRMS) experiments were performed on a microTOF Bruker (electrospray ionization ESI+, 50-1000 in low and 50-2500 in width).
Larue L., Kenzhebayeva B., Al-Thiabat M.G., Jouan-Hureaux V., Mohd-Gazzali A., Wahab H.A., Boura C., Yeligbayeva G., Nakan U., Frochot C, & Acherar S. (2023). tLyp-1: A peptide suitable to target NRP-1 receptor. Bioorganic chemistry, 130.
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3

Synthesis of Blue Fluorescent Compound

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The title compound was synthesized following
the method described in general procedure 3, using 29h (2.3 mg, 2.64 μmol) to afford a blue solid (1.04 mg, 0.80
μmol, 30%). HRMS (Bruker MicroTOF)(m/z): Calculated
(M+H)+ for C69H79B1F2N10O11S1 = 1305.5784; measured
= 1305.5757. Analytical HPLC (system 1): 98% purity; tR= 18.28 min.
Kok Z.Y., Stoddart L.A., Mistry S.J., Mocking T.A., Vischer H.F., Leurs R., Hill S.J., Mistry S.N, & Kellam B. (2022). Optimization of Peptide Linker-Based Fluorescent Ligands for the Histamine H1 Receptor. Journal of Medicinal Chemistry, 65(12), 8258-8288.
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4

Synthesis of Lys-Ala-Asn-Ser-Leu Peptide

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The title compound was synthesized following
the method described in general procedure 1, using Fmoc-Lys(Boc)-OH
(94 mg, 0.20 mmol) for the loading phase; Fmoc-ß-Ala-OH (63 mg,
0.20 mmol), Fmoc-Asn(Trt)-OH (120 mg, 0.20 mmol), Fmoc-Ser(tBu)-OH
(77 mg, 0.20 mmol), and Fmoc-Leu-OH (71 mg, 0.20 mmol) for four separate
coupling phases in the stated sequence; 7 (34 mg, 0.10
mmol) for the capping phase to afford a colorless solid (1.3 mg, 1.53
μmol, 4%). HRMS (Bruker MicroTOF)(m/z): Calculated
(M+H)+ for C43H65N9O9 = 852.4978; measured = 852.4964. Analytical HPLC (system
1): 94% purity; tR = 12.62 min.
Kok Z.Y., Stoddart L.A., Mistry S.J., Mocking T.A., Vischer H.F., Leurs R., Hill S.J., Mistry S.N, & Kellam B. (2022). Optimization of Peptide Linker-Based Fluorescent Ligands for the Histamine H1 Receptor. Journal of Medicinal Chemistry, 65(12), 8258-8288.
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5

Synthesis of Fluorescent Probe Compound

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The title compound was synthesized following
the method described in general procedure 2, using 27a (0.9 mg, 1.06 μmol) to afford a blue solid (0.73 mg, 0.57
μmol, 54%). HRMS (Bruker MicroTOF)(m/z): Calculated
(M+H)+ for C65H76B1F2N13O10S1 = 1280.5693; measured
= 1280.5718. Analytical HPLC (system 1): 98% purity; tR = 15.92 min.
Kok Z.Y., Stoddart L.A., Mistry S.J., Mocking T.A., Vischer H.F., Leurs R., Hill S.J., Mistry S.N, & Kellam B. (2022). Optimization of Peptide Linker-Based Fluorescent Ligands for the Histamine H1 Receptor. Journal of Medicinal Chemistry, 65(12), 8258-8288.
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6

Synthesis of Fluorinated Macrocyclic Compound

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The title compound was synthesized following
the method described in general procedure 2, using 21a (0.5 mg, 0.67 μmol) to afford a blue solid (0.53 mg, 0.45
μmol, 67%). HRMS (Bruker MicroTOF)(m/z): Calculated
(M+H)+ for C60H67B1F2N12O9S1 = 1181.5009; Measured
= 1181.4954; Error = 5.5ppm. Analytical HPLC (system 1): 96% purity; tR = 15.90 min.
Kok Z.Y., Stoddart L.A., Mistry S.J., Mocking T.A., Vischer H.F., Leurs R., Hill S.J., Mistry S.N, & Kellam B. (2022). Optimization of Peptide Linker-Based Fluorescent Ligands for the Histamine H1 Receptor. Journal of Medicinal Chemistry, 65(12), 8258-8288.
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7

Synthesis of Blue Solid Compound

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The title compound was synthesized following
the method described in general procedure 3, using 29b (2.4 mg, 2.92 μmol) to afford a blue solid (1.65 mg, 1.32
μmol, 45%). HRMS (Bruker MicroTOF)(m/z): Calculated
(M+H)+ for C65H78B1F2N11O10S1 = 1254.5788; measured
= 1254.5761. Analytical HPLC (system 1): 98% purity; tR= 18.15 min.
Kok Z.Y., Stoddart L.A., Mistry S.J., Mocking T.A., Vischer H.F., Leurs R., Hill S.J., Mistry S.N, & Kellam B. (2022). Optimization of Peptide Linker-Based Fluorescent Ligands for the Histamine H1 Receptor. Journal of Medicinal Chemistry, 65(12), 8258-8288.
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8

Synthesis of Blue Fluorescent Compound

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The title compound was synthesized following
the method described in general procedure 4, using 29a (1.3 mg, 1.55 μmol) to afford a blue solid (1.28 mg, 0.93
μmol, 60%). HRMS (Bruker MicroTOF)(m/z): Calculated
(M+H)+ for C71H89B1F2N12O12S1 = 1383.6578; measured
= 1383.6513. Analytical HPLC (system 1): 98% purity; tR = 16.31 min.
Kok Z.Y., Stoddart L.A., Mistry S.J., Mocking T.A., Vischer H.F., Leurs R., Hill S.J., Mistry S.N, & Kellam B. (2022). Optimization of Peptide Linker-Based Fluorescent Ligands for the Histamine H1 Receptor. Journal of Medicinal Chemistry, 65(12), 8258-8288.
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9

Solid-Phase Peptide Synthesis of Compound 7

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The title compound was synthesized following
the method described in general procedure 1, using Fmoc-Lys(Boc)-OH
(94 mg, 0.20 mmol) for the loading phase; Fmoc-ß-Ala-OH (63 mg,
0.20 mmol), Fmoc-Tyr(tBu)-OH (80 mg, 0.20 mmol), Fmoc-Ala-OH (63 mg,
0.20 mmol), and Fmoc-Thr(tBu)-OH (68 mg, 0.20 mmol) for four separate
coupling phases in the stated sequence; 7 (34 mg, 0.10
mmol) for the capping phase to afford a colorless solid (2.3 mg, 2.64
μmol, 7%). HRMS (Bruker MicroTOF)(m/z): Calculated
(M+H)+ for C46H64N8O9 = 873.4869; measured = 873.4842. Analytical HPLC (system
1): 98% purity; tR= 12.00
min.
Kok Z.Y., Stoddart L.A., Mistry S.J., Mocking T.A., Vischer H.F., Leurs R., Hill S.J., Mistry S.N, & Kellam B. (2022). Optimization of Peptide Linker-Based Fluorescent Ligands for the Histamine H1 Receptor. Journal of Medicinal Chemistry, 65(12), 8258-8288.
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10

Synthesis of Fluorescent Boron Complex

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The title compound was synthesized following
the method described in general procedure 3, using 29j (1.3 mg, 1.53 μmol) to afford a blue solid (0.59 mg, 0.44
μmol, 29%). HRMS (Bruker MicroTOF)(m/z): Calculated
(M+H)+ for C66H80B1F2N11O11S1 = 1284.5893; measured
= 1284.5844. Analytical HPLC (system 1): 95% purity; tR = 18.03 min.
Kok Z.Y., Stoddart L.A., Mistry S.J., Mocking T.A., Vischer H.F., Leurs R., Hill S.J., Mistry S.N, & Kellam B. (2022). Optimization of Peptide Linker-Based Fluorescent Ligands for the Histamine H1 Receptor. Journal of Medicinal Chemistry, 65(12), 8258-8288.
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