Victor2

Manufactured by PerkinElmer

Sourced in United States, Finland, France, United Kingdom

The Victor2 is a multimode microplate reader designed for a variety of laboratory applications. It provides accurate and reliable measurements of absorbance, fluorescence, and luminescence signals in microplates. The Victor2 is capable of performing various detection modes to support different assay types and experimental requirements.

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Lab products found in correlation

H2dcfda, by Thermo Fisher Scientific (4 mentions) Cell counting kit 8 (cck8), by Dojindo Laboratories (2 mentions) Celltiter glo, by Promega (2 mentions) Dcfh da, by Thermo Fisher Scientific (2 mentions) S30 t7 high yield protein expression system, by Promega (2 mentions) Renilla luciferase assay kit, by Biotium (2 mentions) 96 well white opaque plate, by Greiner (2 mentions) Wst 1 cell proliferation assay kit, by Takara Bio (2 mentions) H2dcfda, by Merck Group (2 mentions) Human apolipoprotein a elisa kit, by Merck Group (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Prism 5, by GraphPad (2 mentions) Lsm 700, by Zeiss (2 mentions) Bicinchoninic acid assay, by Thermo Fisher Scientific (2 mentions) Fluorescent hdac assay kit, by Active Motif (1 mentions) Celltiter blue, by Promega (1 mentions) Apotox glo triplex assay kit, by Promega (1 mentions) Atplite luciferin luciferase kit, by PerkinElmer (1 mentions) Nano luciferase substrate, by Promega (1 mentions) Caspase glo 3 7 assay, by Promega (1 mentions)

Close spelling variants of this product

Wallac Victor2 1420 Multilabel Counter (90 citations) Wallac Victor2 (76 citations) Wallac 1420 Victor2 Microplate Reader (60 citations) Victor2 plate reader (49 citations) Victor2 microplate reader (26 citations) Victor2 Multilabel Counter (24 citations) Wallac 1420 VICTOR2 plate reader (14 citations) Wallac Victor2 plate reader (13 citations) Victor2 luminometer (10 citations) VICTOR2 fluorometry (7 citations)

111 protocols using victor2

Fluorescent HDAC Activity Assay of rAnkA

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rAnkA was tested for HDAC activity using the fluorescent HDAC assay kit (Active Motif, Carlsbad, CA) following the manufacturer's instructions. 5 µg rAnkA was diluted in assay buffer and activity expressed as pmol of fluorescent product formed after incubation at 37°C for 1 h. Fluorescence intensity was measured using a Perkin Elmer Victor II plate reader with an excitation wavelength at 355 nm and emission at 460 nm. HeLa cell extract was used as a positive control.

Rennoll-Bankert K.E., Garcia-Garcia J.C., Sinclair S.H, & Dumler J.S. (2015). Chromatin-bound bacterial effector AnkA recruits HDAC1 and modifies host gene expression. Cellular microbiology, 17(11), 1640-1652.

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Cell Proliferation Inhibition Screening

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Compounds 4-8 were added to Falcon 384 well black/clear tissue treated assay plates containing 3000 adherent cells/well (HEK293) in an assay volume of 45 uL. The plates were incubated for 72 h at 37°C and 5% CO₂. After incubation the supernatant was aspirated from the wells and 40 uL of 10% Alamar Blue added per well. Plates were incubated for a further 5-6 h and measured for fluorescence at 535 nm excitation and 590 nm emission using a VICTOR II (PerkinElmer, Waltham, MA). The % inhibition of cell proliferation was calculated using Dimethylsulfoxide (DMSO) and 10 uM 5-Fluorouracil control data. IC₅₀ values were obtained by plotting % inhibition against log dose using the Prizm4 graphing package and nonlinear regression with variable slope plot.

Wang C., Wan J., Mei Z, & Yang X. (2014). Acridone alkaloids with cytotoxic and antimalarial activities from Zanthoxylum simullans Hance. Pharmacognosy Magazine, 10(37), 73-76.

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Cell Viability and Respiratory Activity Assay

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With this assay, we measured the cell viability and the activity of the respiratory chain. The metabolisation of resazurin to resorufin (CellTiterBlue, Promega, Madison, WI, USA) was captured and, with this, the metabolic activity of the cells. For the CellTiterBlue assay, we plated 3 × 10⁴ cells per well and performed the assay as prescribed in the protocol. Thus, the cells were incubated for sixty minutes with the CellTiterBlue reagent (1 h;1:20 dilution with medium; 400 µL). After this, 2 × 100 μL of the medium/CellTiterBlue reagent solution from each well was transferred to a microtiter plate from each well. The fluorescence spectrometer (Ex/Em = 540/590 nm; VICTOR II, Perkin Elmer, Waltham, MA, USA) was used afterwards to measure cell viability.

Sturm S., Niegisch G., Windolf J, & Suschek C.V. (2024). Exposure of Bladder Cancer Cells to Blue Light (λ = 453 nm) in the Presence of Riboflavin Synergistically Enhances the Cytotoxic Efficiency of Gemcitabine. International Journal of Molecular Sciences, 25(9), 4868.

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Cell Cytotoxicity Assay Protocol

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The cell cytotoxicity was determined after 72 h of treatment using the ApoTox-Glo Triplex Assay kit from Promega (Fitchburg, WI, USA) following the manufacturer's protocol. Fluorescent signal was determined using the plate reader Victor II-PerkinElmer. Values were normalized to ADN content in each well.

Gérard C., Mestdagt M., Tskitishvili E., Communal L., Gompel A., Silva E., Arnal J.F., Lenfant F., Noel A., Foidart J.M, & Péqueux C. (2015). Combined estrogenic and anti-estrogenic properties of estetrol on breast cancer may provide a safe therapeutic window for the treatment of menopausal symptoms. Oncotarget, 6(19), 17621-17636.

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Resazurin Reduction Assay for 3D Spheroid Viability

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To determine the presence of metabolically active cells in our spheroids the resazurin reduction assay was performed [15 (link)]. The assay is based on the principle that resorufin, which is the reduced form of resazurin, can be measured using a fluorescent method, thus indirectly determining the viability rate of incubated spheroids. Following 4 and 14 days of 3D culture, the spheroids were transferred to a non-ULA 96 well plate and incubated for 24 h to allow the spheroids to adhere. After 24 h 100 μL of 10% Prestoblue^® stock solution was added and incubated for a maximum of 24 h at 37 °C under 5% CO₂ humidified conditions. The reduction of the reagent was measured at 595 nm at 2, 4, 6, 8, and 24 h using a multiplate reader (Victor II—Perkin Elmer, Waltham, MA, USA).

Paris F., Marrazzo P., Pizzuti V., Marchionni C., Rossi M., Michelotti M., Petrovic B., Ciani E., Simonazzi G., Pession A., Bonsi L, & Alviano F. (2023). Characterization of Perinatal Stem Cell Spheroids for the Development of Cell Therapy Strategy. Bioengineering, 10(2), 189.

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ATP Measurement in Mitochondria and Cells

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ATP variation in presence of mGluR5 modulators was evaluated in PBS added with ATP 10 µM, in mitochondria and in cell suspensions. ATP was measured by the luminescence method using the ATPlite luciferin/luciferase kit (Perkin Elmer Inc., Waltham, MA, USA). Luminescence was evaluated on a Perkin Elmer Victor II, using a white 96-well plate.

Ferrigno A., Berardo C., Di Pasqua L.G., Siciliano V., Richelmi P., Nicoletti F, & Vairetti M. (2018). Selective Blockade of the Metabotropic Glutamate Receptor mGluR5 Protects Mouse Livers in In Vitro and Ex Vivo Models of Ischemia Reperfusion Injury. International Journal of Molecular Sciences, 19(2), 314.

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Nanoluciferase Assay Protocol

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Ten microliters of sample was added to 10 microliters of nanoluciferase substrate diluted per the protocol (Promega). Luminescence was read 10 minutes after addition with a Victor II (Perkin Elmer).

Shariati K., Pappalardo Z., Chopra D.G., Yiv N., Sheen R, & Ku G. (2020). Selective monitoring of insulin secretion after CRISPR interference in intact pancreatic islets despite submaximal infection. Islets, 12(3), 59-69.

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Caspase-3/7 Activity Assay Protocol

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Caspases-3/7 activity was determined using the Caspase-Glo® 3/7 assay (Promega, Leiden, The Netherlands). 29.4 × 10³ cells/cm² were seeded in 96-well white plates. After 3 days, culture media were replaced by FA-specific medium supplemented with increasing concentrations of Palm, complemented or not with Ole, for the indicated times. At the end of the incubation period, 100 μl of culture medium were replaced by 100 μl of Caspase-Glo reagent resulting in cell lysis and cleavage of the Caspase-Glo® 3/7 substrate by the activated caspases. The luminescent signal generated was detected after 1 h using a Victor2 (PerkinElmer, Zaventem, Belgium).

Dalla Valle A., Vertongen P., Spruyt D., Lechanteur J., Suain V., Gaspard N., Brion J.P., Gangji V, & Rasschaert J. (2019). Induction of Stearoyl-CoA 9-Desaturase 1 Protects Human Mesenchymal Stromal Cells Against Palmitic Acid-Induced Lipotoxicity and Inflammation. Frontiers in Endocrinology, 10, 726.

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Characterization of Stamp2 Promoter Activity

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The 2kb fragment upstream of the putative mouse Stamp2 transcription initiation start site was amplified by PCR using Phusion proofreading polymerase (Finnzymes) and the BAC clone RP23–212F6 (Invitrogen) as a template. The PCR product was inserted into pCR-Blunt II-TOPO (Invitrogen) and sequenced. The 2kb insert was excised out with HindIII and XhoI and ligated into the pGL2-BASIC reporter plasmid (Promega) using the same sites. Empty pcDNA3 and pcDNA3-p50 expression plasmids, or AllStar and NFkB1 or CEBPA (all from Qiagen) siRNA were used in co-transfection experiments as indicated. Lipofectamine 3000 (Invitrogen), FuGene (Roche), or PEI (Sigma-Aldrich) were used as transfection agents. To detect luciferase activity, cells were lysed 48 h post transfection in luciferase lysis buffer (25 mM Tris-HCl, pH 7.8, 2 mM DTT, 10% glycerol, 1% Triton-X). 100 μl luciferin buffer containing 0.2 mM D-Luciferin (Anaspec) and 2 mM ATP, was injected to 10 μl cell extract and luciferase activity was detected by the multiplate reader Victor 2 (PerkinElmer). Activity was normalized to protein concentration.

Sikkeland J., Lindstad T., Zeynep Nenseth H., Dezitter X., Qu S., Muhumed R.M., Erikci Ertunc M., Gregor M.F, & Saatcioglu F. (2019). Inflammation and ER stress differentially regulate STAMP2 expression and localization in adipocytes. Metabolism: clinical and experimental, 93, 75-85.

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Quantifying Osteoclast Activity via TRACP5b

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Serum samples were analyzed for mouse tartrate resistant acid phosphatase 5b (TRACP5b; MouseTRAP, IDS) used as a marker for osteoclast number [18] (link). Five microliters of serum was used for the analysis and the assay was performed according to the instructions provided by the manufacturer. Absorbance was measured using a microplate reader (Victor2, PerkinElmer).

Kähkönen T.E., Tuomela J.M., Grönroos T.J., Halleen J.M., Ivaska K.K, & Härkönen P.L. (2019). Dovitinib dilactic acid reduces tumor growth and tumor-induced bone changes in an experimental breast cancer bone growth model. Journal of Bone Oncology, 16, 100232.

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