Xcalibur data system version 1

MS/MS analyses were performed on an LTQ-OrbitrapXL mass spectrometer (Thermo Fisher Scientific, Monza, Italy) equipped with a nanospray ion source. The spray capillary voltage was set at 1.7 kV, and the ion transfer capillary temperature was held at 220 °C. Full MS spectra were recorded over a 400–1600 m/z range in positive ion mode, with a resolving power of 60,000 (full width at half-maximum) and a scan rate of 2 spectra/s. This step was followed by five low-resolution MS/MS events that were sequentially generated in a data-dependent manner on the top five ions selected from the full MS spectrum (at 35% of collision energy), using dynamic exclusion of 0.5 min for MS/MS analysis. Mass spectrometer scan functions and high-performance liquid chromatography solvent gradients were controlled by the Xcalibur data system version 1.4 (Thermo Fisher Scientific, Monza, Italy).

MS/MS analyses were performed on an LTQ-OrbitrapXL mass spectrometer (Thermo Fisher Scientific, Monza, Italy) equipped with a nanospray ion source. The spray capillary voltage was set at 1.7 kV and the ion transfer capillary temperature was held at 220 °C. Full MS spectra were recorded over a 400–1600 m/z range in positive ion mode, with a resolving power of 60000 (full width at half-maximum) and a scan rate of 2 spectra/s. This step was followed by five low-resolution MS/MS events that were sequentially generated in a data-dependent manner on the top five ions selected from the full MS spectrum (at 35% collision energy), using dynamic exclusion of 0.5 min for MS/MS analysis. Mass spectrometer scan functions and high-performance liquid chromatography solvent gradients were controlled by the Xcalibur data system version 1.4 (Thermo Fisher Scientific, Monza, Italy).