Delta t dishes

Manufactured by Bioptechs

Sourced in United States

The Delta T dishes are a type of lab equipment designed for live-cell imaging. They provide a controlled environment for maintaining cells at a specified temperature during microscopy observations.

Automatically generated - may contain errors

Lab products found in correlation

Axiocam hrm ccd camera, by Zeiss (3 mentions) Lsm 710, by Zeiss (3 mentions) Femtotip, by Eppendorf (2 mentions) Ziessaxiovert 200 m microscope, by Zeiss (2 mentions) Axiovert 200m microscope, by Zeiss (1 mentions) Bx51wi microscope, by Olympus (1 mentions) Sylgard, by Dow (1 mentions) Umplfln20xw, by Olympus (1 mentions) Umplfln 40xw, by Olympus (1 mentions) Umplfln10xw, by Olympus (1 mentions) Injectman, by Eppendorf (1 mentions) Cb dex, by Thermo Fisher Scientific (1 mentions) Femtojet pump, by Eppendorf (1 mentions) Cos 7 cells, by American Type Culture Collection (1 mentions) Sodium pyruvate, by Thermo Fisher Scientific (1 mentions) Dmem hg medium, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Glutamax, by Thermo Fisher Scientific (1 mentions) X tremegene hp dna transfection reagent, by Roche (1 mentions) Glutamax, by American Type Culture Collection (1 mentions)

Close spelling variants of this product

Delta T culture dish controller (10 citations) Delta-T dish (7 citations) Delta T (5 citations) Delta T culture dish (4 citations) Delta T Open Dish System (4 citations) Delta T culture dishes (4 citations) Delta T heated culture dishes (2 citations)

22 protocols using delta t dishes

Live-cell Imaging of Glioblastoma Cell Motility

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were plated on laminin-coated Bioptechs delta-T dishes. For the duration of video acquisition, cells were maintained in a sealed chamber at 37 °C and 5% CO₂. Phase-contrast images were taken at 10-min intervals for 90 min total. Images were acquired with the 10× objective of a Zeiss Axiovert 200M microscope equipped with an AxioCam HRm CCD camera (Zeiss). Motility was quantitated using the MtrackJ plugin (42 (link)) in ImageJ software (National Institutes of Health) as described previously (15 (link)). For live cell imaging and video microscopy of actin, lentiviral particles expressing eGFP-tagged Lifeact (23 (link)) were made and used to transduce glioblastoma cells. pLenti Lifeact-EGFP BlastR was a gift from Ghassan Mouneimne (Addgene plasmid # 84383).

Lavictoire S.J., Jomaa D., Gont A., Jardine K., Cook D.P, & Lorimer I.A. (2021). Identification of Rac guanine nucleotide exchange factors promoting Lgl1 phosphorylation in glioblastoma. The Journal of Biological Chemistry, 297(5), 101172.

+ Open protocol

+ Expand

Bovine Ampullae Inflammation Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Qualitative and quantitative analyses in bovine ampullae showing mild, moderate or severe inflammation (n = 45) as well as in healthy controls (n = 10) were performed by digital live cell imaging. 1 cm part of the ampulla of each sample was cut and opened longitudinally. Specimens were pinned onto a Delta T dishes (Bioptechs Inc., PA, USA) coated with Sylgard® (Dow Corning, MI, USA). All samples were maintained at 37.5 °C using a stage heater (Delta T Controller, Bioptech Inc., USA) and an objective lens heater (Delta T Controller, Bioptech Inc., USA) attached to the microscope. Reference temperature was monitored prior to all recordings. For sperm-oviduct interaction and average survival time, 600,000 sperm (30 μl) were coincubated with the ampulla for 10 mins at 37 °C and submerged with sperm Talp before analysis. Imaging was performed with a fixed stage microscope, upright Olympus microscope (BX51WI) with water immersion dipping objectives equipped with the bright-field long-distance immersion objectives UMPLFLN 10xW, UMPLFLN 20xW and UMPLFLN 40xW (Olympus, Hamburg, Germany). Images and videos were documented with a SUMIX Mx7 camera (Summix, CA, USA).

Owhor L.E., Reese S, & Kölle S. (2019). Salpingitis Impairs Bovine Tubal Function and Sperm-Oviduct Interaction. Scientific Reports, 9, 10893.

+ Open protocol

+ Expand

Microinjection-based Titration and Activity Assay for miRNA

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells grown on DeltaT dishes (Bioptechs, # 0420042105C) were microinjected as described (Pitchiaya et al., 2012 (link); Pitchiaya et al., 2013 (link), Pitchiaya et al., 2017 (link)). Briefly, injection solutions contained the appropriate miRNA at 1 μM concentration, 1x PBS and 0.5 mg/mL of 10 kDa cascade blue conjugated dextran (CB-Dex, Thermo-Fisher, # D1976). For microinjection based titration assays solution with 0 – 0.1 μM, 1x PBS and 0.1 mg/mL of 500 kDa cascade blue conjugated dextran (FITC-Dex, Thermo-Fisher, # D7136). For microinjection based miRNA activity assay, mRNAs were added at a stoichiometric amount based on the number of miRNA binding sites, for instance, 0.16 μM of RL-cx6x mRNA, bearing 6 cxcr4 binding sites, was added along with 1 μM cxcr4 miRNA. Solutions were filtered through a 0.45 μm Ultrafree-MC filter (Millipore, # UFC30HV00) and then centrifuged at 16,000 × g for 15min at 4 °C immediately before injection. The solution was loaded into a femtotip (Eppendorf, # E5242952008). Injections were performed using a Femtojet pump (Eppendorf) and an Injectman (Eppendorf) mounted to the microscope. Microinjections were performed at 100 hPa injection pressure for 0.5 s with 20 hPa compensation pressure. This pressure translates to a volume of 0.02 pL and 10,000–20,000 miRNA molecules.

Pitchiaya S., Mourao M.D., Jalihal A., Xiao L., Jiang X., Chinnaiyan A.M., Schnell S, & Walter N.G. (2019). Dynamic recruitment of single RNAs to processing bodies depends on RNA functionality. Molecular cell, 74(3), 521-533.e6.

+ Open protocol

+ Expand

Measuring Cell Migration Velocity

Check if the same lab product or an alternative is used in the 5 most similar protocols

A total of 1.5 × 10⁵ INCs were plated on to poly-l-Lysine coated (10 µg/mL in H₂O) Delta T dishes (Bioptechs). Cells were grown one day to become a monolayer. The following day, the media was changed to Cell Imaging Solution (20 mM Hepes, pH 7.4, 2.5 mM KCl, 140 mM NaCl, 5 mM glucose, 1.4 mM CaCl₂, 0.5 mM MgCl₂) with 10% FBS following the scratch to image without the need for 5% CO₂ and incubated at 37°C for 20 min. The Delta T dish was then mounted into the stage holder and a scratch was made on the dish with a p20 pipette tip. The Delta T dishes were kept at 37°C in the Biotechs DeltaT stage holder using a heated cover. Time lapse images of multiple locations were taken every 5 min for 6 h at 60× magnification as cells moved into the wound. Cell velocity was calculated using the ImageJ cell tracking software MTrackJ to track the distance individual cells traveled over the 6-h time window (Meijering et al. 2012 (link)). Cell velocities were plotted using box and whisker plots. For all box and whisker plots, the thick black line indicates the median, the box indicates the interquartile range, and the whiskers indicate the range.

Lampasona A.A, & Czaplinski K. (2019). Hnrnpab regulates neural cell motility through transcription of Eps8. RNA, 25(1), 45-59.

+ Open protocol

+ Expand

Imaging of dNK-Trophoblast Interactions

Check if the same lab product or an alternative is used in the 5 most similar protocols

JEG-3 were cultured in Delta T dishes (Bioptechs) coated with fibronectin (20 μg/ml), which were adapted to an Olympus FV1000 4-channel Confocal Imaging system. dNK were pre-labeled with Vybrant DiO at 1:1000 for 30 min in serum-free RPMI and then washed 3 times. After temperature calibration (37°C), the JEG-3 Delta dish was placed in the stage, and dNK were added at T=0. Cells were imaged with the 60x objective with transmitted light and 488 nm laser with emission filter of 500–545 nm, and images were taken every minute for up to 20 minutes. Images were processed using FV10-ASW software version 03.00.01.15.

Crespo Â.C., Mulik S., Dotiwala F., Ansara J.A., Santara S.S., Ingersoll K., Ovies C., Junqueira C., Tilburgs T., Strominger J.L, & Lieberman J. (2020). Decidual NK cells transfer granulysin to selectively kill bacteria in trophoblasts. Cell, 182(5), 1125-1139.e18.

+ Open protocol

+ Expand

COS-7 Cell Culture and Transfection

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell culture was performed as reported previously^{23 (link)} and reiterated here. COS-7 cells were obtained from ATCC (Manassas, VA, USA, catalog number CRL-1651) and maintained in standard DMEM-HG medium supplemented with 10% (vol/vol) heat-inactivated FBS, 1 mM sodium pyruvate and 2 mM Glutamax in a 5% CO₂ incubator at 37 ^°C. For imaging, the cells were plated in Bioptechs Delta-T dishes (Bioptechs, Butler, PA, USA, product number 04200417B) and grown to ~ 70% confluency. Transient transfections were done with various plasmids according to manufacturer’s instructions using 1–2 μg DNA and 3ul of X-tremeGene HP DNA transfection reagent (Roche, Manheim, Germany, product number 06366236001). The transfected cells were incubated at 37 °C and 5% CO₂ incubator for 24–48 h before imaging. The cell culture reagents DMEM-HG medium (catalog number 11960), fetal bovine serum (FBS, catalog number 10082), sodium pyruvate (catalog number 11360) and Glutamax (catalog number 35050) were obtained from Invitrogen (Life Technologies, Grand Island, NY, USA). Calyculin A was obtained from Sigma (catalog number C 5552).

Ojha N., Rainey K.H, & Patterson G.H. (2020). Imaging of fluorescence anisotropy during photoswitching provides a simple readout for protein self-association. Nature Communications, 11, 21.

+ Open protocol

+ Expand

Live Cell Protein Microinjection Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols

NIH-3T3 cells (1 × 10⁵) were seeded onto delta-T dishes (Bioptechs) and transfected with the vector expressing BFP-LacI one day before microinjection. The regular DMEM was replaced with phenol red-free medium 4 h prior to microinjection. Immediately before microinjection, the cell culture medium was replaced with a minimal HEPES buffered saline (HBS) medium without serum and vitamins, but containing 20 mM HEPES–KOH (pH 7.4), 135 mM NaCl, 5 mM KCl, 1 mM MgCl₂, 1.8 mM CaCl₂ and 5.6 mM glucose. The micropipette (Femtotip, Eppendorf) was loaded with ∼0.5 amol fluorophore-labeled proteins. Twenty fg of I-SceI and Trex2 were also added to the microinjection system. Live cells with a BFP dot were selected for imaging. We used HILO illumination to image cells at 120× magnification with 30 ms camera exposure on two Andor iXon Ultra EMCCD cameras using a cell-TIRF system on an Olympus IX81 microscope.

Yang G., Liu C., Chen S.H., Kassab M.A., Hoff J.D., Walter N.G, & Yu X. (2018). Super-resolution imaging identifies PARP1 and the Ku complex acting as DNA double-strand break sensors. Nucleic Acids Research, 46(7), 3446-3457.

+ Open protocol

+ Expand

Live-cell Motility Quantification Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were directly plated on laminin coated Bioptechs delta-T dishes (Butler, PA, USA) in 1mL media. During image acquisition cells were maintained in sealed chambers at 37°C. Phase-contrast images were taken at 10 minute intervals for 1-1.3 hours using a 10x objective. Images were acquired using a ZiessAxiovert 200 M microscope equipped with a AxioCamHRm CCD camera (Zeiss, Göttingen, Germany). Motility was quantified using the MtrackJ plugin [49 (link)] in ImageJ software (National Institutes of Health, Bethesda, Maryland, USA) and scored as the average distance to point per cell per frame.

Gont A., Daneshmand M., Woulfe J., Lavictoire S.J, & Lorimer I.A. (2016). PREX1 integrates G protein-coupled receptor and phosphoinositide 3-kinase signaling to promote glioblastoma invasion. Oncotarget, 8(5), 8559-8573.

+ Open protocol

+ Expand

Microinjection of Sea Urchin Eggs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Adult Strongylocentrotus purpuratus were collected in La Jolla, CA, and held at 11°C (± 1°C) in flow-through seawater aquaria. Spawning and gamete gathering was performed as described previously (Campanale and Hamdoun, 2012 (link)). For microinjection, eggs were held on protamine sulfate coated delta-T dishes (Bio-ptechs, Butler, PA), and mRNA was injected at 2–5% of egg volume (Lepage and Gache, 2004 (link)). Injected embryos were cultured in filtered seawater (FSW) containing 100 µg/ml ampicillin (Sigma, St Louis, MO) at 15°C (± 0.5°C) for 24 hr, then plucked and cultured to gastrula stage in FSW lacking ampicillin.

Campanale J.P., Gökirmak T., Espinoza J.A., Oulhen N., Wessel G.M, & Hamdoun A. (2014). Migration of Sea Urchin Primordial Germ Cells. Developmental dynamics : an official publication of the American Association of Anatomists, 243(7), 917-927.

+ Open protocol

+ Expand

Microinjection of Cells on DeltaT Dishes

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells grown on DeltaT dishes (Bioptechs) were microinjected as described (Pitchiaya et al., 2012 (link); Pitchiaya et al., 2013 (link)).

Pitchiaya S., Heinicke L.A., Park J.I., Cameron E.J, & Walter N.G. (2017). Resolving sub-cellular miRNA trafficking and turnover at single-molecule resolution. Cell reports, 19(3), 630-642.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!