Openlab cds

The results from the mycotoxin analyses were performed using the software package OpenLab CDS (Agilent Technologies, Santa Clara, CA, USA). A sample was considered positive for a tested mycotoxin when the concentration was above the limit of detection (LD). Samples below the LD (non-detects or left-censored data) were assigned a value of one-half the LD for the interpretation of the results and exposure assessment purposes [23 ]. The descriptive analysis of the mean, standard deviation (SD) and relative standard deviation (RSD%) was performed with Statistical Package SPSS v21 (IBM Corporation, Armonk, NY, USA).

The GC (7890B, Agilent, Singapore) used in this study was equipped with flame ionization detector (FID) and capillary column (HP-MOLESIEVE, 0.530 mm × 30 m × 25.0 μm, Agilent, Singapore). The workstations for software manipulation and data processing were OpenLAB CDS developed by Agilent. According to the referenced standard (HJ 38-2017) [30 (link)], the temperature of the column chamber, gasification chamber and detector was 80 °C, 100 °C and 200 °C, respectively. Nitrogen (99.999%, Min Xing Gong Mao Co., Ltd., Hangzhou, China) was used as the carrier gas, and the total flow rate was set at 30 mL min⁻¹. The carrier gas was then divided into two channels, in which the flow of the make-up gas was controlled at 22 mL min⁻¹, and the other flow of the capillary column was controlled at 8 mL min⁻¹. Air flow (99.999%, Min Xing Gong Mao Co., Ltd., Hangzhou, China) and hydrogen flow (99.999%, Jin Gong Special Gas Co., Ltd., Hangzhou, China) were set at 300 mL min⁻¹ and 30 mL min⁻¹, respectively. The automatic injection pattern mentioned in the standard was replaced by manual injection based on the existing conditions in the laboratory.

Purifications were performed on an Agilent Infinity system equipped with an Infinity 1260 Bio Quat Pump (pump system, G5611A), an Infinity 1260 HiP Bio ALS (autosampler, G1330B), an Infinity 1290 TCC (thermostatted column compartment, G5667A), an Infinity 1260 DAD (diode array detector, G4212B), and an Infinity 1260 Bio FC-AS (fraction collector, G5664A) under Agilent OpenLab CDS (C.01.07 SR1) software control. The column (Waters XBridge BEH C18 OBD, 130 Å, 5 µm, 10 × 150 mm) was kept at 50 °C. Material was injected as aqueous solutions (up to 100 µL per injection) and eluted with gradients of solvent A (50 mM triethylammonium acetate in water) and solvent B (MeOH). Fraction collection was triggered by UV (260 nm). The following 5–50% method was used: flow 5.0 mL/min, runtime 20 min, column used was Waters XBridge BEH C18 OBD, 130 Å, 5 µm, 10 × 150 mm. The run conditions were as follows: 0.0–15.0 min 95% solvent A, 5% solvent B; 15.0–16.0 min 50% solvent A, 50% solvent B; 16.0–18.1 min 5% solvent A, 95% solvent B; 18.1–20.0 min 95% solvent A, 5% solvent B.
Following separation, desired species-containing fractions were pooled and concentrated by ultrafiltration (Millipore Amicon Ultra-15/4 centrifugal filters; Ultracel 10 K/3 K) according to manufacturer guidelines. Three cycles of sample concentration/buffer dilution with nuclease-free water were employed.