Anti ccp2 elisa

Manufactured by Axis-Shield

Sourced in United Kingdom

The Anti-CCP2 ELISA is a laboratory test kit used to detect the presence of anti-cyclic citrullinated peptide (anti-CCP) antibodies in human serum or plasma samples. This test is designed to aid in the diagnosis of rheumatoid arthritis.

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Lab products found in correlation

Bio plex platform, by Bio-Rad (1 mentions)

2 protocols using anti ccp2 elisa

Quantitative 14-3-3η Biomarker Assessment

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Baseline plasma was assessed for 14-3-3η levels using the quantitative 14-3-3η enzyme-linked immunosorbent assay (ELISA, Augurex Life Sciences Corp, Vancouver, Canada). Positivity for 14-3-3η was defined as ≥0.19 ng/ml based on the manufacturer's recommended cut-off, and at 2 times and 4 times this cut-off. The development, validation and calibration of the assay are detailed in a recent publication [6 (link)]. ACPA was measured by an anti-CCP2 ELISA (Axis Shield, Dundee, UK) and immunoglobulin M rheumatoid factor (IgM-RF) by an in-house ELISA as described previously [10 (link)]. The cut-off level for ACPA positivity was set at ≥5 arbitrary units/ml (AU/ml), according to the manufacturer's instructions. The cut-off level for IgM-RF positivity was set at ≥30 international units/ml (IU/ml).

van Beers-Tas M.H., Marotta A., Boers M., Maksymowych W.P, & van Schaardenburg D. (2016). A prospective cohort study of 14-3-3η in ACPA and/or RF-positive patients with arthralgia. Arthritis Research & Therapy, 18, 76.

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Multi-Biomarker Evaluation in Rheumatoid Arthritis

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ACPA was measured using a second-generation anti-cyclic citrullinated peptide (anti-CCP2) ELISA (Diastat, Axis-Shield, Dundee, Scotland, UK; positive test ≥ 5 U/ml). Both rheumatoid factor (RF; positive ≥ 15 IU/ml) and high sensitivity C-reactive protein (hs-CRP, mg/L) were determined by nephelometry (Siemens Healthcare Diagnostics, Munich, Germany). Serum samples were also evaluated for 19 specific ACPA using a bead-based multiplex antigen array on the BioPlex platform (30 (link)), an array measuring disease-specific autoantibody reactivity to multiple citrullinated autoantigens. An ACPA score was calculated as the sum of normalized fluorescent values divided by 19 (the number of ACPA tested). Other disease characteristics assessed included tender and swollen joint counts (0 to 28) in addition to both provider and patient global well-being scores (0 to 100 mm visual analog scales or VAS). A 28-joint Disease Activity Score (DAS-28-CRP) was calculated (31 (link)). Standard posterior-anterior hand and wrist radiographs were obtained in RA cases and scored by a single investigator (AE) blinded to PD status using the modified Sharp scoring method (32 (link)).

Mikuls T.R., Payne J.B., Yu F., Thiele G.M., Reynolds R.J., Cannon G.W., Markt J., McGowan D., Kerr G.S., Redman R.S., Reimold A., Griffiths G., Beatty M., Gonzalez S., Bergman D.A., Hamilton B.C., Erickson A.R., Sokolove J., Robinson W., Walker C., Chandad F, & O’Dell J.R. (2014). Periodontitis and Porphyromonas gingivalis in Patients with Rheumatoid Arthritis. Arthritis & rheumatology (Hoboken, N.J.), 66(5), 1090-1100.

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