Mircury lna anti mir 193b

Trypsin, Dulbecco's Modified Eagle Medium (DMEM), MEM vitamins, MEM nonessential amino acids and Penicillin-Streptomycin were purchased from Media Tech (Manassas, VA). TaqMan miRNA assay for hsa-miR-193b was obtained from Applied Biosystems (Foster City, CA). pre-miR-193b, and scrambled pre-miR negative control were from Ambion (Austin, TX), miRCURY LNA anti-miR-193b and scrambled anti-miR negative control were from Exiqon (Vedbæk, Denmark). The lentivirus vector expressing copepod (c) GFP (CD511B-1) and pre-miR-193b, miR-Zip-193b microRNA inhibitor vector, the control vectorsand the lentivirus packaging kit (LV500A-1) were purchased from System Biosciences (Mountain View, CA). The DNMT inhibitor 5-Aza-2′-deoxycytidine (decitabine) was purchased from Sigma-Aldrich, St. Louis, MO (Cat# A3656). The siRNA's used were Dharmacon (Lafayette, CO) SMARTpool siRNAs: DNMT1 siRNA M-004605-01 and siGENOME PLAU siRNA M-006000-02

OvCa cells were transfected^{14 (link)} with 30 nM pre-miR-193b, scrambled control oligo (Ambion), 30 nM miRCURY LNA anti-miR-193b, or scrambled control LNA oligo (Exiqon) using siPORTNeoFX (Ambion). The cells were used for experiments 48 h after transfection or as indicated.